冰冻PRP和冻干PRP的抗菌作用研究*

doi:10.3969/j.issn.1671-2587.2022.06.005

临床输血与检验 ›› 2022, Vol. 24 ›› Issue (6): 705-709.DOI: 10.3969/j.issn.1671-2587.2022.06.005

冰冻PRP和冻干PRP的抗菌作用研究^*

毛平平, 王淑君, 罗开云, 齐清, 赵广超, 栾建凤

210002 南京,解放军东部战区总医院输血医学科（毛平平,王淑君,罗开云,齐清,赵广超,栾建凤）; 东南大学医学院（王淑君）

收稿日期:2022-06-17 发布日期:2023-01-05
通讯作者: 王淑君,女,主治医师,硕士,主要从事输血治疗相关研究,（E-mail）shujun0106@sina.com。栾建凤,女,主任技师,硕士,主要从事输血和相关装备研究,（E-mail）luanjf1003@126.com。
作者简介:毛平平（1989-）,女,江苏东台人,技师,本科,主要从事血液成分制备工作,（E-mail）543055928@qq.com。
基金资助:
*本课题受江苏省卫健委面上项目（No.H2019110）、东部战区总医院军事医学面上课题（No.YYMS2021019）资助

Study on the Antibacterial Effect of Frozen Platelet-rich Plasma and Lyophilized Platelet-rich Plasma

MAO Ping-ping, WANG Shu-jun, LUO Kai-yun, et al

Department Blood Transfusion Medicine, Jinling Hospital Nanjing University, School Medicine, Nanjing 210002

Received:2022-06-17 Published:2023-01-05

摘要/Abstract

摘要： 目的探讨富血小板血浆（platelet-rich plasma,PRP）冰冻和冻干形式对四种临床常见感染菌有无抑制作用,并比较抑菌作用差异。方法使用血细胞单采机采集新鲜血小板,制备成冰冻PRP和冻干PRP。以冰冻PRP和冻干PRP为实验组、生理盐水作为对照组,采用涂布平板计数法计数,观察不同时间点各组对金黄色葡萄球菌、大肠杆菌、白色念珠菌、铜绿假单胞菌生长是否有抑制作用。结果对比生理盐水组,冰冻PRP组和冻干PRP组均对金黄色葡萄球菌、大肠杆菌和白色念珠菌的生长有抑制作用且有显著差异;对铜绿假单胞菌无抑制作用且促进其生长。在有抑菌效果的各时间点,冰冻PRP组与冻干PRP组对金黄色葡萄球菌、大肠杆菌和白色念珠菌的抑制作用有显著差异。结论冰冻PRP和冻干PRP均具有抑菌作用,且有特定的抑菌谱和时效性,冻干PRP比冰冻PRP具有更强的作用。

关键词: 富血小板血浆, 冷冻, 冻干, 抑菌实验

Abstract: Objective To investigate the inhibitory efficacy of platelet-rich plasma (PRP) in frozen and lyophilized forms against four common infectious bacteria and to compare the differences in inhibition. Methods Fresh platelets from healthy volunteers were collected by blood apheresis machine and prepared into frozen PRP and lyophilized PRP, which as experimental groups. We set saline as control group and observed whether above 3 groups could inhibit the growth of Staphylococcus aureus (S. aureus), Escherichia coli (E. coli) and Candida albicans (C. albicans) and Pseudomonas aeruginosa (P. aeruginosa) at different time points by coated plate counting method. Results Compared to saline group, both frozen PRP and lyophilized PRP groups inhibited the growth of S. aureus, E. coli and C. albicans, and increased P. aeruginosa growth. At each point, frozen PRP and lyophilized PRP groups showed significant inhibitory effects against S. aureus, E. coli and C. albicans. There were significant differences in bacteriostasis at some points between the frozen PRP and lyophilized PRP groups. Conclusion Both frozen PRP and lyophilized PRP have antibacterial effects with specific antibacterial spectrum and timeliness, and lyophilized PRP has stronger effects than frozen PRP.

Key words: Platelet-rich plasma, Frozen, Lyophilized, Bacteriostatic test

中图分类号:

R457

毛平平, 王淑君, 罗开云, 齐清, 赵广超, 栾建凤. 冰冻PRP和冻干PRP的抗菌作用研究^*[J]. 临床输血与检验, 2022, 24(6): 705-709.

MAO Ping-ping, WANG Shu-jun, LUO Kai-yun, et al. Study on the Antibacterial Effect of Frozen Platelet-rich Plasma and Lyophilized Platelet-rich Plasma[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2022, 24(6): 705-709.

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冰冻PRP和冻干PRP的抗菌作用研究^*

Study on the Antibacterial Effect of Frozen Platelet-rich Plasma and Lyophilized Platelet-rich Plasma

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