miR-532-3p靶向SERPINE1调控细胞外基质受体互作通路抑制结肠腺癌细胞增殖和迁移

doi:10.3969/j.issn.1671-2587.2023.03.007

临床输血与检验 ›› 2023, Vol. 25 ›› Issue (3): 324-333.DOI: 10.3969/j.issn.1671-2587.2023.03.007

miR-532-3p靶向SERPINE1调控细胞外基质受体互作通路抑制结肠腺癌细胞增殖和迁移

刘挺, 文燕平

425000 永州市中心医院北院胃肠外科（刘挺）; 永州市中心医院北院超声科（文燕平）

收稿日期:2023-03-10 发布日期:2023-07-10
通讯作者: 文燕平,主要从事超声影像学研究,（E-mail）wenxiancca@163.com。
作者简介:刘挺,主要从事胃肠外科研究,（E-mail）liutiting12@163.com。

MiR-532-3p Targets SERPINE1 and Regulates ECM RECEPTOR INTERACTION to Inhibit Proliferation and Migration of Colon Adenocarcinoma Cells

LIU Ting, WEN Yanping

Department of Gastrointestinal Surgery, The Central Hospital of Yongzhou, Yongzhou 425000

Received:2023-03-10 Published:2023-07-10

摘要/Abstract

摘要： 目的本研究旨在明确miR-532-3p/SERPINE1在结肠腺癌中的具体调控机制。方法利用TCGA数据库筛选差异表达的mRNA,随后通过mirDIP、miRWalk数据库分析并确定上游调控基因miRNA。GSEA数据库进行富集通路的分析。实时荧光定量聚合酶链式反应（Real-time quantitative PCR,qRT-PCR）、细胞计数试剂盒（Cell Counting Kit-8,CCK-8）实验、克隆形成实验、划痕愈合、Transwell、蛋白质免疫印迹（Western blot,WB）研究SERPINE1及上游基因对结肠腺癌增殖、迁移、侵袭的调控机制。结果 SERPINE1在结肠腺癌细胞和组织中显著高表达,而miR-532-3p呈显著低表达。SERPINE1与结肠腺癌患者的预后不良有关,能促进结肠腺癌细胞增殖、迁移和侵袭。SERPINE1显著富集在细胞外基质受体互作通路。miR-532-3p靶向负调控SERPINE1的表达,回复实验表明miR-532-3p/SERPINE1调控细胞外基质受体互作通路影响结肠腺癌细胞的恶性进展。结论 miR-532-3p作为SERPINE1的上游调控基因,通过调控细胞外基质受体互作通路抑制结肠腺癌细胞增殖、迁移和侵袭。

关键词: 结肠腺癌, SERPINE1, miR-532-3p, 增殖, 迁移

Abstract: Objective SERPINE1 is associated with poor prognosis of many cancers and plays an important role in tumor metastasis. This study aims to clarify the specific regulatory mechanism of miR-532-3p/SERPINE1 in colon adenocarcinoma. Methods TCGA database was used to screen differentially expressed mRNAs, and then mirDIP and miRWalk databases were used to analyze and identify upstream regulatory gene miRNAs. GSEA database was used to the analysis of enrichment pathways. Real time quantitative polymerase chain reaction (qRT-PCR), CCK-8, cloning experiment, scratch healing, Transwell, Western blot (WB) to study the regulatory mechanism of SERPINE1 and its upstream genes on proliferation, migration and invasion of colon adenocarcinoma. Results SERPINE1 was significantly overexpressed in colon adenocarcinoma cells and tissues, while miR-532-3p was significantly overexpressed. SERPINE1 is related to the poor prognosis of colon adenocarcinoma patients and can promote the proliferation, migration and invasion of colon adenocarcinoma cells. SERPINE1 was significantly enriched in ECM RECEPTOR INTERACTION pathway. MiR-532-3p targets and negatively regulates the expression of SERPINE1. The reversion experiment shows that miR-532-3p/SERPINE1 regulates the ECM RECEPTOR INTERACTION pathway and affects the malignant progression of colon adenocarcinoma cells. Conclusion As the upstream regulatory gene of SERPINE1, miR-532-3p inhibits the proliferation, migration and invasion of colon adenocarcinoma cells by regulating ECM RECEPTOR INTERACTION. MiR-532-3p/SERPINE1 has the potential to become a new target for the treatment of colon adenocarcinoma.

Key words: Adenocarcinoma of colon, SERPINE1, miR-532-3p, Proliferation, Transfer

中图分类号:

R735.3

刘挺, 文燕平. miR-532-3p靶向SERPINE1调控细胞外基质受体互作通路抑制结肠腺癌细胞增殖和迁移[J]. 临床输血与检验, 2023, 25(3): 324-333.

LIU Ting, WEN Yanping. MiR-532-3p Targets SERPINE1 and Regulates ECM RECEPTOR INTERACTION to Inhibit Proliferation and Migration of Colon Adenocarcinoma Cells[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2023, 25(3): 324-333.

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miR-532-3p靶向SERPINE1调控细胞外基质受体互作通路抑制结肠腺癌细胞增殖和迁移

MiR-532-3p Targets SERPINE1 and Regulates ECM RECEPTOR INTERACTION to Inhibit Proliferation and Migration of Colon Adenocarcinoma Cells

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