改良法制备冰冻解冻去甘油红细胞初步应用研究

doi:10.3969/j.issn.1671-2587.2023.04.013

临床输血与检验 ›› 2023, Vol. 25 ›› Issue (4): 498-502.DOI: 10.3969/j.issn.1671-2587.2023.04.013

改良法制备冰冻解冻去甘油红细胞初步应用研究

陈婷婷, 卓海龙, 刘慧, 王道琳, 骆群

解放军总医院第五医学中心输血医学科,北京 100071

收稿日期:2023-06-01 出版日期:2023-08-20 发布日期:2023-09-18
通讯作者: 骆群,主任医师,博士,主要从事免疫血液学,重症疑难患者输血,治疗性单采及全血转换术等方面研究,（E-mail）luoq66@aliyun.com。
作者简介:陈婷婷,主要从事临床输血等方面研究,（E-mail）653218122@qq.com。

A Modified Method for Frozen Thawed Glycerolized Red Blood Cells: A Preliminary Study

CHEN Tingting, ZHUO Hailong, LIU Hui, et al

Department of Transfusion, Fifth Medical Center of Chinese PLA General Hospital, Beijing, 100071

Received:2023-06-01 Online:2023-08-20 Published:2023-09-18

摘要/Abstract

摘要： 目的探究将用于制备冰冻解冻去甘油红细胞的氯化钠溶液体系替换成枸橼酸钠溶液体系和甘氨酸溶液体系,并对洗涤后的产品进行质量检测及分析评价。方法选择 7 d内去白细胞悬浮红细胞10袋,甘油化后至-80℃±5℃制备成冰冻红细胞产品,保存6个月。将其等量分为对照组和实验组,每组各10袋。对照组为原有制备方法,实验组解冻第1次洗涤的9% NaCl替换成终浓度为18.75%甘氨酸溶液体系,第2次及后续洗涤液的0.9% NaCl替换终浓度为2.58%的枸橼酸钠体系。记录两组制备时间,比较两组冰冻解冻去甘油红细胞的质量指标,并进行分析评价。结果技术改良后缩短了冰冻解冻去甘油红细胞的制备时间。与对照组相比,终产品红细胞回收率无明显差异,无统计学意义（P>0.05）;上清游离血红蛋白含量、甘油残留量含量下降,有统计学意义（P<0.05）。ATP和2,3-DPG含量明显升高,有统计学意义（P<0.05）。结论通过改良冰冻解冻去甘油红细胞制备方法,使红细胞产品质量持续稳定,并更优化,能进一步提高临床患者血液输注的安全性、有效性和时效性。

关键词: 冰冻红细胞, 枸橼酸钠, 甘氨酸, 溶血率, 回收率

Abstract: Objective The sodium chloride (NaCl) solution used for the preparation of frozen thawed glycerolized red blood cells (RBCs) was replaced by sodium citrate and glycine solutions. The quality of washed RBCs was analyzed. Methods Ten bags of suspended leukocyte-depleted RBCs collected within 7 days were selected, glycerolized and prepared into frozen RBCs, and stored at -80℃±5℃ for 6 months. They were divided into control group and experimental group, each group 10 bags. In the control group, the original preparation method was used. In the experimental group, the 9% NaCl in the first washing was replaced by 18.75% glycine solution, and the 0.9% NaCl in the second and subsequent washing solution was replaced by 2.58% sodium citrate solution. The preparation time of the two groups was recorded, and the quality indexes were compared and analyzed. Results The preparation time of glycerol removal from frozen thawed RBCs was shortened by improved methods. Compared with the control group, there was no significant difference in the recovery rate of RBCs (P >0.05). The contents of free hemoglobin and residual glycerin in supernatant decreased, with statistical significance (P< 0.05). The levels of ATP and 2, 3-DPG were significantly increased, with statistical significance (P<0.05). Conclusion By improved preparation method of frozen deglycerolized RBCs, the quality of RBCs can be stable and optimized. This method can further improve the safety, effectiveness and timeliness of blood transfusion for patients.

Key words: Frozen red blood cells, Sodium citrate, Glycine, Hemolysis ratio, Percent recovery

中图分类号:

R457

陈婷婷, 卓海龙, 刘慧, 王道琳, 骆群. 改良法制备冰冻解冻去甘油红细胞初步应用研究[J]. 临床输血与检验, 2023, 25(4): 498-502.

CHEN Tingting, ZHUO Hailong, LIU Hui, et al. A Modified Method for Frozen Thawed Glycerolized Red Blood Cells: A Preliminary Study[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2023, 25(4): 498-502.

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改良法制备冰冻解冻去甘油红细胞初步应用研究

A Modified Method for Frozen Thawed Glycerolized Red Blood Cells: A Preliminary Study

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