关键词: 血小板输注无效, 人类白细胞抗原, 相对免疫原性

Abstract: Objective To analyze the specificity of HLA-I antibody in patients with platelet transfusion refractoriness. Methods Samples from 65 patients with platelet transfusion refractoriness were collected. Luminex platform was used to screen platelet-related antibodies and identify the specificity of HLA-I antibodies. The relative immunogenicity of different alleles was calculated according to the frequency of HLA-I alleles in Henan Han population reported in the laboratory and the number of positive antibodies detected in this experiment. Results HLA-I antibodies were detected in 51 of 65 samples including HLA-A antibodies and HLA-B antibodies. The antibody positive rates of A*11:01 and B*13:02 with the highest alleles frequencies of HLA-A and B loci were 0.33 and 0.65, respectively, and the relative immunogenicity was 0.113 0and0.183 8. The allele frequency of A^*34:01 in Henan population was only 0.000 4, but the relative immunogenicity of corresponding antigen was as high as 48.795 3. Similarly, the allele frequency of B^*44:02 is only 0.000 1, but the relative immunogenicity of its corresponding antigen is up to 127.420 4. Conclusion The characteristics of HLA-I antibodies are different in the patients with platelet transfusion refractoriness, and the immunogenicity of their corresponding antigens is different. The analysis of antibody characteristics and antigen relative immunogenicity can provide data support for the selection of appropriate donors for the patients, improve the infusion effect, and prevent the production of new antibodies.

Key words: Platelet transfusion refractoriness, Human leukocyte antigen, Relative immunogenicity