关键词: 血浆, 乳糜程度, 冰点冷却倒置离心法

Abstract: Objective The aim of this study is to explore the efficiency of freezing point cooling inverted centrifugation on plasma chylemia reduction. Methods 46 bags of frozen plasma (200 mL/bag) were collected from blood composition preparation process. The freezing point cooling inverted centrifugation was employed for removal of chylemia from plasma, and the absorbance, lipid levers, total plasma protein (TP) and chylemia were analyzed before or after the centrifugation to evaluate the effect of chylemia reduction. Results After the treatment of freezing point cooling inverted centrifugation, the OD value, TG content and chylous index of were significantly decreased in moderate chylous plasma (P<0.01). While, the levels of TC, HDL, LDL and TP were not significantly different from those without treatment (P>0.05). The OD value, TG content and chylous index were significantly decreased in severe chylous plasma after treatment of freezing point cooling inverted centrifugation (P<0.01). The appearance of plasma was significant improved, and the compliance rate of comprehensive standards was 75%. However, the levels of TC, HDL, LDL and TP were not significantly different from those without treatment (P>0.05). Conclusion Freezing point cooling inverted centrifugation method can effectively reduce plasma chyle degree, improve the compliance rate of plasma quality control, reduce the scrap rate of blood and minimize the waste of blood resources as much as possible.

Key words: Plasma, Chyle degree, Freezing point cooling inverted centrifugation method