水凝胶负载PRP治疗慢性难愈性创面的研究进展*

doi:10.3969/j.issn.1671-2587.2023.04.027

临床输血与检验 ›› 2023, Vol. 25 ›› Issue (4): 564-571.DOI: 10.3969/j.issn.1671-2587.2023.04.027

水凝胶负载PRP治疗慢性难愈性创面的研究进展^*

王自林¹, 刘宏杰¹, 张娅¹, 祝丽丽²

¹贵州医科大学,贵阳 550001;
²贵州医科大学附属医院输血科; 贵州医科大学附属白云医院输血科

收稿日期:2023-05-05 出版日期:2023-08-20 发布日期:2023-09-18
通讯作者: 祝丽丽,主任技师,博士,主要从事临床输血方面研究,（E-mail）zhulili2000@gmc.edu.cn。
作者简介:王自林,主要从事临床检验诊断学方面研究,（E-mail）1973507222@qq.com。
基金资助:
*本课题受贵州省卫健委项目（No.gzwkj2021-366）、贵阳市科技局项目筑科合（No.[2018]1-75）资助

Hydrogel-loaded PRP for the Treatment of Chronic Refractory Wounds

WANG Zilin, LIU Hongjie, ZHANG Ya, et al

School of Clinical Laboratory Science, Guizhou Medical University, Guiyang Guizhou Province 550001

Received:2023-05-05 Online:2023-08-20 Published:2023-09-18

摘要/Abstract

摘要： 慢性难愈性创面是临床治疗中的一个严峻难题。尽管富血小板血浆（platelet rich plasma,PRP）治疗已被广泛证实为有效的治疗方法,但传统的PRP体外激活后在短时间内会释放全部的生长因子,并且因激活后胶体与上清液分离,富含因子的上清液容易流淌丢失,难以形成稳定的结构,影响了其治疗效果。为了克服这一问题,近年来研究人员开始探索使用水凝胶作为PRP的载体,以改善传统PRP治疗的缺陷。因此本文综述了水凝胶搭载PRP治疗慢性难愈性创面的最新研究进展,并为优化治疗方案提供了参考。

关键词: 富血小板血浆, 水凝胶, 慢性难愈性创面

Abstract: The treatment of chronic non-healing wounds remain challenging in terms of complexity. Although platelet rich plasma (PRP) therapy has been widely proven effective, the traditional method of in vitro activation of PRP leads to the rapid release of all growth factors. Due to the separation of colloid and supernatant after activation, the factor-rich supernatant is easy to flow and lose, and it is difficult to form a stable structure, which affects its therapeutic effect. To overcome this problem, researchers in recent years have explored hydrogels as carriers for PRP to improve the shortcomings of traditional PRP treatment. This article reviews the latest research on hydrogel-loaded PRP for the treatment of chronic non-healing wounds and provides a reference for optimal treatment.

Key words: Platelet rich plasma, Hydrogel, Chronic refractory wounds

中图分类号:

R457

王自林, 刘宏杰, 张娅, 祝丽丽. 水凝胶负载PRP治疗慢性难愈性创面的研究进展^*[J]. 临床输血与检验, 2023, 25(4): 564-571.

WANG Zilin, LIU Hongjie, ZHANG Ya, et al. Hydrogel-loaded PRP for the Treatment of Chronic Refractory Wounds[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2023, 25(4): 564-571.

参考文献

[1] SCHILRREFF P,ALEXIEV U.Chronic inflammation in non-healing skin wounds and promising natural bioactive compounds treatment[J].Int J Mol Sci,2022,23(9):4928.
[2] TANAKA R,INOUE H,ISHIKAWA T,et al.Use of sponge-foam inserts in compression bandaging of non-healing venous leg ulcers[J].Ann Vasc Dis,2021,14(1):46-51.
[3] RODRÍGUEZ-RODRÍGUEZ N,MARTÍNEZ-JIMÉNEZ I,GARCÍA-OJALVO A,et al.Wound chronicity,impaired immunity and infection in diabetic patients[J].MEDICC Rev,2021,24(1):44-58.
[4] LI S X,MOHAMEDI A H,SENKOWSKY J,et al.Imaging in chronic wound diagnostics[J].Adv Wound Care,2020,9(5):245-263.
[5] ALVEN S,ADERIBIGBE B A.Chitosan and cellulose-based hydrogels for wound management[J].Int J Mol Sci,2020,21(24):9656.
[6] 谭谦,徐晔.慢性创面治疗的理论和策略[J].中华烧伤杂志,2020,36(9):798-802.
[7] WANG Y H, WANG Z L, et al.ZHANG Y.Platelet-Rich Plasma Reduces LPS-Stimulated Inflammatory Reaction in BV2 Cells by Blocking the TLR4-Mediated NF-κB/MAPKs Pathway[J].Journal of Biological Regulators and Homeostatic Agents,2023, 37(4):2159-2170
[8] EVERTS P,ONISHI K,JAYARAM P,et al.Platelet-rich plasma:new performance understandings and therapeutic considerations in 2020[J].Int J Mol Sci,2020,21(20):7794.
[9] EVERTS P A,VAN ERP A,DESIMONE A,et al.Platelet rich plasma in orthopedic surgical medicine[J].Platelets,2021,32(2):163-174.
[10] SIMONPIERI A,DEL CORSO M,VERVELLE A,et al.Current knowledge and perspectives for the use of platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) in oral and maxillofacial surgery part 2:bone graft,implant and reconstructive surgery[J].Curr Pharm Biotechnol,2012,13(7):1231-1256.
[11] OPNEJA A,KAPOOR S,STAVROU E X.Contribution of platelets,the coagulation and fibrinolytic systems to cutaneous wound healing[J].Thromb Res,2019,179:56-63.
[12] GOLEBIEWSKA E M,POOLE A W.Platelet secretion:from haemostasis to wound healing and beyond[J].Blood Rev,2015,29(3):153-162.
[13] JOHN A E,WHITE N J.Platelets and fibrinogen:emerging complexity in trauma-induced coagulopathy[J].Semin Thromb Hemost,2020,46(2):125-133.
[14] UCHIYAMA R,TOYODA E,MAEHARA M,et al.Effect of platelet-rich plasma on M1/M2 macrophage polarization[J].Int J Mol Sci,2021,22(5):2336.
[15] PICCIN A,DI PIERRO A M,CANZIAN L,et al.Platelet gel:a new therapeutic tool with great potential[J].Blood Transfus,2017,15(4):333-340.
[16] 付丹妹,孙诚,陈建民,等.负压创面治疗联合富血小板血浆治疗慢性难愈性创面的研究进展[J].医学研究生学报,2022,35(2):196-201.
[17] PHILLIPSON M,KUBES P.The healing power of neutrophils[J].Trends Immunol,2019,40(7):635-647.
[18] AGGOUR R L,GAMIL L.Antimicrobial effects of platelet-rich plasma against selected oral and periodontal pathogens[J].Pol J Microbiol,2017,66(1):31-37.
[19] LIU X L,YANG Y L,NIU X,et al.An in situ photocrosslinkable platelet rich plasma - Complexed hydrogel glue with growth factor controlled release ability to promote cartilage defect repair[J].Acta Biomater,2017,62:179-187.
[20] GOODWIN N S,SPINKS A,WASIAK J.The efficacy of hydrogel dressings as a first aid measure for burn wound management in the pre-hospital setting:a systematic review of the literature[J].Int Wound J,2016,13(4):519-525.
[21] ZHANG L J,YIN H X,LEI X,et al.A systematic review and meta-analysis of clinical effectiveness and safety of hydrogel dressings in the management of skin wounds[J].Front Bioeng Biotechnol,2019,7:342.
[22] HO T C,CHANG C C,CHAN H P,et al.Hydrogels:proper ti es and applications in biomedicine[J].Molecules,2022,27(9):2902.
[23] OSI A R,ZHANG H,CHEN J,et al.Three-dimensional-printable thermo/photo-cross-linked methacrylated chitosan-gelatin hydrogel composites for tissue engineering[J].ACS Appl Mater Interfaces,2021,13(19):22902-22913.
[24] LEI X X,HU J J,ZOU C Y,et al.Multifunctional two-component in situ hydrogel for esophageal submucosal dissection for mucosa uplift,postoperative wound closure and rapid healing[J].Bioact Mater,2023,27:461-473.
[25] ITAI S,ONOE H.Flexibly deformable collagen hydrogel tube reproducing immunological tissue deformation of blood vessels as a pharmacokinetic testing model (adv.healthcare mater.1/2022)[J].Adv Healthc Mater,2022,11(1):2270005.
[26] LUECKGEN A,GARSKE D S,ELLINGHAUS A,et al.Hydrolytically-degradable click-crosslinked alginate hydrogels[J].Biomaterials,2018,181:189-198.
[27] FRIEND N E,MCCOY A J,STEGEMANN J P,et al.A combination of matrix stiffness and degradability dictate microvascular network assembly and remodeling in cell-laden poly(ethylene glycol) hydrogels[J].Biomaterials,2023,295:122050.
[28] YANG K,ZHOU X Y,LI Z L,et al.Ultrastretchable,self-healable,and tissue-adhesive hydrogel dressings involving nanoscale tannic acid/ferric ion complexes for combating bacterial infection and promoting wound healing[J].ACS Appl Mater Interfaces,2022,14(38):43010-43025.
[29] GARCIA-ORUE I,SANTOS-VIZCAINO E,SANCHEZ P,et al.Bioactive and degradable hydrogel based on human platelet-rich plasma fibrin matrix combined with oxidized alginate in a diabetic mice wound healing model[J].Mater Sci Eng C Mater Biol Appl,2022,135:112695.
[30] SUN Z H,XIONG H,LOU T F,et al.Multifunctional extracellular matrix hydrogel with self-healing properties and promoting angiogenesis as an immunoregulation platform for diabetic wound healing[J].Gels,2023,9(5):381.
[31] MA S Q,HU H,WU J Z,et al.Functional extracellular matrix hydrogel modified with MSC-derived small extracellular vesicles for chronic wound healing[J].Cell Prolif,2022,55(4):e13196.
[32] SONG F Y,KONG Y,SHAO C Y,et al.Chitosan-based multifunctional flexible hemostatic bio-hydrogel[J].Acta Biomater,2021,136:170-183.
[33] FAROKHI M,MOTTAGHITALAB F,FATAHI Y,et al.Overview of silk fibroin use in wound dressings[J].Trends Biotechnol,2018,36(9):907-922.
[34] GYEONGWOO L,GWANG K Y,HYUN B K,et al.Green tea catechin-grafted silk fibroin hydrogels with reactive oxygen species scavenging activity for wound healing applications[J].Biomater Res,2022,26(1):62.
[35] HEMMATGIR F,KOUPAEI N,POORAZIZI E.Characterization of a novel semi-interpenetrating hydrogel network fabricated by polyethylene glycol diacrylate/polyvinyl alcohol/tragacanth gum as a wound dressing[J].Burns,2022,48(1):146-155.
[36] LAURANO R,BOFFITO M,ABRAMI M,et al.Dual stimuli-responsive polyurethane-based hydrogels as smart drug delivery carriers for the advanced treatment of chronic skin wounds[J].Bioact Mater,2021,6(9):3013-3024.
[37] POGOSTIN B H,YU M H,AZARES A R,et al.Multidomain peptide hydrogel adjuvants elicit strong bias towards humoral immunity[J].Biomater Sci,2022,10(21):6217-6229.
[38] LOPEZ-SILVA T L,CRISTOBAL C D,EDWIN LAI C S,et al.Self-assembling multidomain peptide hydrogels accelerate peripheral nerve regeneration after crush injury[J].Biomaterials,2021,265:120401.
[39] ZHANG W L,LIU Y N,XUAN Y,et al.Synthesis and applications of carboxymethyl cellulose hydrogels[J].Gels,2022,8(9):529.
[40] WEI L,TAN J Y,LI L,et al.Chitosan/alginate hydrogel dressing loaded FGF/VE-cadherin to accelerate full-thickness skin regeneration and more normal skin repairs[J].Int J Mol Sci,2022,23(3):1249.
[41] CATOIRA M C,FUSARO L,DI FRANCESCO D,et al.Overview of natural hydrogels for regenerative medicine applications[J].J Mater Sci Mater Med,2019,30(10):115.
[42] OUYANG Q Q,HU Z,LIN Z P,et al.Chitosan hydrogel in combination with marine peptides from tilapia for burns healing[J].Int J Biol Macromol,2018,112:1191-1198.
[43] OUYANG Q Q,HOU T T,LI C P,et al.Construction of a composite sponge containing tilapia peptides and chitosan with improved hemostatic performance[J].Int J Biol Macromol,2019,139:719-729.
[44] VENKATESAN J,PALLELA R,BHATNAGAR I,et al.Chitosan-amylopectin/hydroxyapatite and chitosan-chondroitin sulphate/hydroxyapatite composite scaffolds for bone tissue engineering[J].Int J Biol Macromol,2012,51(5):1033-1042.
[45] OUYANG Q Q,KONG S Z,HUANG Y M,et al.Preparation of nano-hydroxyapatite/chitosan/tilapia skin peptides hydrogels and its burn wound treatment[J].Int J Biol Macromol,2021,181:369-377.
[46] BARRERA J A,TROTSYUK A A,MAAN Z N,et al.Adipose-derived stromal cells seeded in pullulan-collagen hydrogels improve healing in murine burns[J].Tissue Eng A,2021,27(11/12):844-856.
[47] YANG X F,YANG H Y,JIANG X Y,et al.Injectable chitin hydrogels with self-healing property and biodegradability as stem cell carriers[J].Carbohydr Polym,2021,256:117574.
[48] JAVANBAKHT S,NABI M,SHADI M,et al.Carboxymethyl cellulose/tetracycline@UiO-66 nanocomposite hydrogel films as a potential antibacterial wound dressing[J].Int J Biol Macromol,2021,188:811-819.
[49] WU J A,HOU S Y,REN D C,et al.Antimicrobial properties of nanostructured hydrogel webs containing silver[J].Biomacromolecules,2009,10(9):2686-2693.
[50] 何勍,吴钢,余波,等.伤口愈合水凝胶治疗慢性下肢静脉曲张性溃疡的早期结果[J].中国修复重建外科杂志,2008,22(3):311-313.
[51] 刘震杰,符伟国.静脉性溃疡的敷料选用[J].中国普外基础与临床杂志,2009,16(6):439-440.
[52] BASTIANCICH C,VANVARENBERG K,UCAKAR B,et al.Lauroyl-gemcitabine-loaded lipid nanocapsule hydrogel for the treatment of glioblastoma[J].J Control Release,2016,225:283-293.
[53] UHART M,PIROT F,BOILLON A,et al.Assessment of sodium hyaluronate gel as vehicle for intracameral delivery of cefuroxime in endophthalmitis prophylaxis[J].Int J Pharm,2010,398(1/2):14-20.
[54] DUMAN G,ASLAN İ,YEKTA ÖZER A,et al.Liposome,g el and lipogelosome formulations containing sodium hyaluronate[J].J Liposome Res,2014,24(4):259-269.
[55] QIAO Z Q,ZHANG W J,WU Y M,et al.Host defense peptide-mimicking peptide polymer-based antibacterial hydrogel enables efficient healing of MRSA-infected wounds[J].Sci China Chem,2023,66(6):1824-1833.
[56] HUANG K,LIU W B,WEI W Y,et al.Photothermal hydrogel encapsulating intelligently bacteria-capturing bio-MOF for infectious wound healing[J].ACS Nano,2022,16(11):19491-19508.
[57] JIANG Y F,RAN X W,JIA L J,et al.Epidemiology of type 2 diabetic foot problems and predictive factors for amputation in China[J].Int J Low Extrem Wounds,2015,14(1):19-27.
[58] XU N,WANG L L,GUAN J J,et al.Wound healing effects of a Curcuma zedoaria polysaccharide with platelet-rich plasma exosomes assembled on chitosan/silk hydrogel sponge in a diabetic rat model[J].Int J Biol Macromol,2018,117:102-107.
[59] MORIMOTO N,KAKUDO N,MATSUI M,et al.Exploratory clinical trial of combination wound therapy with a gelatin sheet and platelet-rich plasma in patients with chronic skin ulcers:study protocol[J].BMJ Open,2015,5(5):e007733.
[60] PEREZ-LOPEZ S,PEREZ-BASTERRECHEA M,GARCIA-GALA J M,et al.Stem cell and tissue engineering approaches in pressure ulcer treatment[J].J Spinal Cord Med,2023,46(2):194-203.
[61] LU K,LI K K,ZHANG M,et al.Adipose-derived stem cells (ADSCs) and platelet-rich plasma (PRP) loaded gelatin/silk fibroin hydrogels for improving healing in a murine pressure ulcer model[J].Chem Eng J,2021,424:130429.
[62] 许育兵,刘广亚,施琳颖,等.载血小板凝胶上清复合可吸收敷料对大鼠烧伤创面的修复作用[J].中国输血杂志,2019,32(11):1102-1105.
[63] ZHU X R,KAZEMI A,DONG Y Q,et al.Effectiveness of nano bioactive glass fiber loaded with platelet-rich plasma on thermal wound healing process in rats[J].J Biomed Nanotechnol,2022,18(2):535-545.
[64] MUTHUSWAMY S,VISWANATHAN A,YEGAPPAN R,et al.Antistaphylococcal and neutrophil chemotactic injectable κ-carrageenan hydrogel for infectious wound healing[J].ACS Appl Bio Mater,2019,2(1):378-387.
[65] WEI S K,XU P C,YAO Z X,et al.A composite hydrogel with co-delivery of antimicrobial peptides and platelet-rich plasma to enhance healing of infected wounds in diabetes[J].Acta Biomater,2021,124:205-218.
[66] SHI L Y,LIN F,ZHOU M,et al.Preparation of biocompatible wound dressings with dual release of antibiotic and platelet-rich plasma for enhancing infected wound healing[J].J Biomater Appl,2021,36(2):219-236.

水凝胶负载PRP治疗慢性难愈性创面的研究进展^*

Hydrogel-loaded PRP for the Treatment of Chronic Refractory Wounds

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 13

编辑推荐

Metrics

本文评价

关于本刊

作者中心

在线期刊

访问统计

联系我们

[1]	肖军, 雷慧芬, 李翠莹. 血小板裂解液与血小板凝胶生长因子谱的表达差异研究^*[J]. 临床输血与检验, 2023, 25(4): 450-455.
[2]	中国输血协会临床输血管理学专业委员会. 自体单采富血小板血浆治疗薄型子宫内膜的专家共识[J]. 临床输血与检验, 2023, 25(3): 289-296.
[3]	张强, 岳宪虎, 李瑞, 黄象艳. 富血小板血浆在髌骨外侧高压症治疗中的应用^*[J]. 临床输血与检验, 2023, 25(3): 348-353.
[4]	刘琴, 沈先涛, 徐春芬, 王阳, 杨柳春, 胡红兵. 自体富血小板血浆治疗儿童病理性骨折疗效观察^*[J]. 临床输血与检验, 2023, 25(3): 354-357.
[5]	巩蜜蜜, 汪海宁, 吴磊, 邵小宝, 周琳, 杨圆, 王华, 高凤威, 戴宇东, 朱培元. 同种异体富血小板血浆用于慢性难愈性创面的临床研究^*[J]. 临床输血与检验, 2023, 25(3): 368-374.
[6]	毛平平, 王淑君, 罗开云, 齐清, 赵广超, 栾建凤. 冰冻PRP和冻干PRP的抗菌作用研究^*[J]. 临床输血与检验, 2022, 24(6): 705-709.
[7]	魏燕, 刘宽, 李祥军, 彭娟, 王瑞含, 刘久波. PRP治疗踝关节创伤性滑膜炎疗效观察^*[J]. 临床输血与检验, 2022, 24(6): 725-729.
[8]	罗开云, 王淑君, 毛平平, 孙杨子, 齐清, 栾建凤. 不同保存方式对PRP相关生长因子影响的研究*[J]. 临床输血与检验, 2022, 24(1): 70-73.
[9]	杨菁, 周谋, 林放, 施琳颖, 李艳辉, 李文丹, 单桂秋. 负载PRP壳聚糖-明胶复合敷料止血和促愈合效果的初步评价^*[J]. 临床输血与检验, 2021, 23(5): 612-616.
[10]	杨晓亚, 高裕华, 刘素蕊, 李烛. 冰冻保存PRP对IL-1β诱导的软骨细胞炎症反应的影响[J]. 临床输血与检验, 2021, 23(4): 442-445.
[11]	林放, 周谋, 单桂秋, 李艳辉, 李文丹. 不同来源复水液对冻干PRP活化和释放生长因子影响的比较^*[J]. 临床输血与检验, 2021, 23(2): 142-145.
[12]	王瑞含, 范金波, 周国均, 王飞, 刘久波. PRP治疗膝骨关节炎的临床应用研究进展[J]. 临床输血与检验, 2020, 22(6): 659-663.
[13]	王淑君, 赵广超, 栾建凤, 朱培元. 不同规格全血制备富血小板血浆的最佳离心条件研究^*[J]. 临床输血与检验, 2017, 19(2): 119-122.