沉默MIB2抑制甲状腺乳头状癌细胞的实验研究*

doi:10.3969/j.issn.1671-2587.2026.03.006

临床输血与检验 ›› 2026, Vol. 28 ›› Issue (3): 332-338.DOI: 10.3969/j.issn.1671-2587.2026.03.006

沉默MIB2抑制甲状腺乳头状癌细胞的实验研究^*

樊瑞军¹, 王羽佳¹, 谢文华¹, 贾咏存¹, 郭临池²

¹宁夏回族自治区人民医院,宁夏银川 750002;
²三峡大学附属仁和医院,湖北宜昌 443000

收稿日期:2025-12-29 出版日期:2026-06-20 发布日期:2026-07-07
通讯作者: 郭临池,主要从事内分泌疾病方面研究,（E-mail）Glinchi@163.com。
作者简介:樊瑞军,主要从事病原微生物学与肿瘤分子生物学研究,（E-mail）frj.7373@163.com。
基金资助:
^{^*}本课题受宁夏自然科学基金（No.2023AAC03455）; 2023年肿瘤微环境与免疫治疗湖北省重点实验室（三峡大学）开放基金（No.2023KZL033）; 宜昌市医疗卫生研究项目（No.A24-2-045）; 湖北省自然科学基金（No.2024AFC040）资助

MIB2 Knockdown Exerts Tumor-suppressive Effects in Papillary Thyroid Carcinoma Cells

FAN Ruijun¹, WANG Yujia¹, XIE Wenhua¹, JIA Yongcun¹, GUO Linchi²

¹People's Hospital of Ningxia Hui Autonomous Region, Yinchuan 750002;
²SANXIA University, Yichang 443000

Received:2025-12-29 Online:2026-06-20 Published:2026-07-07

摘要/Abstract

摘要： 目的通过生物信息学分析发现MIB2在甲状腺乳头状癌（PTC）中呈现高表达,并可能是PTC发生中的关键分子,本文研究进一步探讨沉默MIB2对甲状腺乳头状细胞（TPC-1）增殖、凋亡、侵袭与迁移的影响。方法以TPC-1甲状腺乳头状癌细胞为研究对象,筛选沉默MIB2稳定株,并将细胞分为TPC-1组和TPC-1-MIB2 RNAi组,采用CCK8法及克隆形成实验检测沉默MIB2对TPC-1细胞的增殖情况、Transwell侵袭实验及细胞划痕实验检测沉默MIB2对TPC-1细胞侵袭能力的影响、流式细胞术检测沉默MIB2对TPC-1细胞凋亡及周期的影响。结果相较于对照组,TPC-1-MIB2 RNAi组细胞增殖的速度显著降低,说明沉默MIB2抑制了甲状腺乳头状癌细胞的增殖（P<0.05）。流式细胞术显示,TPC-1-MIB2 RNAi组的细胞主要停滞在G1/G0期且TPC-1-MIB2 RNAi组相较于TPC-1组细胞凋亡显著增多,表明沉默MIB2促进了甲状腺乳头状癌细胞的凋亡（P<0.05）。Transwell侵袭实验、细胞划痕实验显示,TPC-1-MIB2 RNAi组细胞的侵袭和迁移能力明显低于TPC-1组,且细胞愈合速度显著减慢,表明沉默MIB2抑制了甲状腺乳头状癌细胞的侵袭与迁移能力（P<0.05）。结论沉默MIB2能抑制甲状腺乳头状癌细胞增殖、侵袭与迁移,并促进甲状腺乳头状癌细胞凋亡,提示MIB2有可能成为甲状腺乳头状癌的潜在治疗靶点。

关键词: 甲状腺乳头状细胞, MIB2, 增殖, 凋亡, 侵袭与迁移

Abstract: Objective Bioinformatic analyses have identified aberrant overexpression of E3 ubiquitin ligase Mindbomb 2 (MIB2) in papillary thyroid carcinoma (PTC), implicating it as a key driver of PTC tumorigenesis. This study characterizes the functional impacts of MIB2 silencing on proliferation, apoptosis, invasion, and migration in the PTC-derived TPC-1 cell line. Methods MIB2-silenced TPC-1 cell lines were generated via RNA interference (RNAi). Cells were divided into control (TPC-1) and MIB2-silenced (TPC-1-MIB2 RNAi) groups. Cell proliferative capacity was quantified using Cell Counting Kit-8 (CCK-8) and colony formation assays. Invasive and migratory potentials were evaluated via Transwell invasion and wound healing assays, respectively. Flow cytometry was performed to assess cell cycle distribution and apoptotic rates. Results Compared with control cells, TPC-1-MIB2 RNAi group exhibited significantly attenuated proliferation kinetics and reduced colony formation efficiency, consistent with robust suppression of PTC cell proliferation following MIB2 knockdown (P<0.05). Flow cytometric profiling revealed prominent G0/G1 phase cell cycle arrest in TPC-1-MIB2 RNAi group, coupled with a marked elevation in apoptotic rate compared with control cells, indicative of enhanced PTC cell apoptosis mediated by MIB2 silencing (P<0.05). Transwell invasion and wound healing assays demonstrated that TPC-1-MIB2 RNAi cells markedly impaired the invasive and migratory capacities of TPC-1 cells, as evidenced by reduced transmembrane invasion and significantly delayed wound closure compared to TPC-1 group (P<0.05). Conclusion MIB2 silencing exerts potent tumor-suppressive effects in PTC cells through inhibition of proliferation, invasion, and migration, alongside concomitant promotion of apoptosis. These findings identify MIB2 as a promising candidate therapeutic target for papillary thyroid carcinoma.

Key words: Papillary thyroid carcinoma, Mindbomb 2 (MIB2), Cell proliferation, Apoptosis, Tumor invasion and migration

中图分类号:

R736.1

樊瑞军, 王羽佳, 谢文华, 贾咏存, 郭临池. 沉默MIB2抑制甲状腺乳头状癌细胞的实验研究^*[J]. 临床输血与检验, 2026, 28(3): 332-338.

FAN Ruijun, WANG Yujia, XIE Wenhua, JIA Yongcun, GUO Linchi. MIB2 Knockdown Exerts Tumor-suppressive Effects in Papillary Thyroid Carcinoma Cells[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2026, 28(3): 332-338.

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沉默MIB2抑制甲状腺乳头状癌细胞的实验研究^*

MIB2 Knockdown Exerts Tumor-suppressive Effects in Papillary Thyroid Carcinoma Cells

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