雷帕霉素干预急性肺损伤SD大鼠NF-κB p65和STAT-1基因表达研究*

doi:10.3969/j.issn.1671-2587.2016.03.002

临床输血与检验 ›› 2016, Vol. 18 ›› Issue (3): 204-208.DOI: 10.3969/j.issn.1671-2587.2016.03.002

雷帕霉素干预急性肺损伤SD大鼠NF-κB p65和STAT-1基因表达研究*

李丽玮, 李志强

200233 上海交通大学附属第六人民医院

收稿日期:2016-01-15 出版日期:2016-06-20 发布日期:2016-09-21
通讯作者: 李志强（1962-）,男,主任医师,教授,硕士研究生导师,主要从事血液系统疾病诊治与临床输血不良反应研究,（Tel）021-24058722（E-mail）kcb039@126.com。
作者简介:李丽玮（1987-）,女,江苏扬州人,技师,硕士,主要从事临床输血不良反应研究,（Tel）021-24058703（E-mail）alice_517@foxmail.com。
基金资助:
*本课题受上海市科学技术委员会科研计划项目基金资助（No.13140902903); 第四轮（2015-2017年）上海市公共卫生三年行动计划重点建设项目《输血医学》资助

The Influence of Rapamycin on NF-κB p65 and STAT-1 Expression in Rats with Acute Lung Injury

LI Li-Wei, LI Zhi-qiang

The Sixth People’Hospital of Shanghai Jiaotong University, Shanghai 200233

Received:2016-01-15 Online:2016-06-20 Published:2016-09-21

摘要/Abstract

摘要： 目的研究雷帕霉素对输血相关急性肺损伤（TRALI）和急性胰腺炎相关性肺损伤（APALI）的SD大鼠肺组织核转录因子p65 (NF-κB p65)和信号转导与转录激活子-1(STAT-1)基因相对表达量的影响。方法将SD大鼠60只随机分成6组,分别为TRALI组、APALI组、雷帕霉素干预TRALI组、雷帕霉素干预APALI组、雷帕霉素干预对照组、正常对照组,每组10只。应用实时荧光定量RT-PCR检测大鼠肺组织NF-κB p65和STAT-1基因相对表达量,分析雷帕霉素对TRALI、APALI在mTOR相关细胞凋亡过程的干预作用。结果相比正常对照组,TRALI及APALI组大鼠肺组织NF-κB p65和STAT-1基因相对表达量均有不同程度下降（P<0.05）,且APALI组下降程度比TRALI组更明显（P<0.01）;雷帕霉素干预后,TRALI组NF-κB p65和STAT-1基因相对表达量与TRALI组比较均进一步下降（P<0.01）,而APALI组和对照组下降则不明显或无变化（P>0.05）。结论应用雷帕霉素干预治疗TRALI大鼠可能会进一步加重其肺损伤的程度,而雷帕霉素对APAL大鼠则影响作用有限。

关键词: 急性肺损伤, 输血, 胰腺炎, 基因, NF-κ, B, p65, STAT-1

Abstract: ObjectiveTo study the NF-κB p65 and STAT-1 expression in Sprague Dawley rats with transfusion-related acute lung injury or acute pancreatitis associated lung injury. MethodSixty SD rats were randomly divided into TRALI group, APALI group, control group, rapamycin treated TRALI group, rapamycin treated APALI group, and rapamycin treated control group, with 10 animals in each. Gene expression of NF-κB p65 and STAT-1 was detected by qRT-PCR. The method of 2-△△Ct was used to calculate the relative qualification of target genes. ResultThe expression of NF-κB p65 and STAT-1 in TRALI and APALI groups was found to be down-regulated（P<0.05）, and APALI group decreased more than TRALI group（P<0.01）. The gene expression of NF-κB p65 and STAT-1 in rapamycin traeted TRALI group decreased more than in TRALI group（P<0.01）, while rapamycin intervention APALI group did not decrease significantly compared to APALI group（P>0.05）. ConclusionRapamycin might deteriorate pathological process of the lung injury in TRALI, but have limited effects on APALI.

Key words: Acute, lung, injury, Transfusion, Pancreatitis, Gene, NF-κB, p65, STAT-1

中图分类号:

R563R392.12

李丽玮, 李志强. 雷帕霉素干预急性肺损伤SD大鼠NF-κB p65和STAT-1基因表达研究*[J]. 临床输血与检验, 2016, 18(3): 204-208.

LI Li-Wei, LI Zhi-qiang. The Influence of Rapamycin on NF-κB p65 and STAT-1 Expression in Rats with Acute Lung Injury[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2016, 18(3): 204-208.

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雷帕霉素干预急性肺损伤SD大鼠NF-κB p65和STAT-1基因表达研究*

The Influence of Rapamycin on NF-κB p65 and STAT-1 Expression in Rats with Acute Lung Injury

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