富血小板纤维蛋白上清改善大鼠特应性皮炎的实验研究*

doi:10.3969/j.issn.1671-2587.2023.01.012

临床输血与检验 ›› 2023, Vol. 25 ›› Issue (1): 69-74.DOI: 10.3969/j.issn.1671-2587.2023.01.012

富血小板纤维蛋白上清改善大鼠特应性皮炎的实验研究^*

王巧改, 毕利博, 刘素蕊

050082 联勤保障部队第九八〇医院检验科（王巧改）,联勤保障部队第九八〇医院输血医学科（毕利博）,联勤保障部队三亚康复疗养中心检验病理科（刘素蕊）

收稿日期:2022-09-16 发布日期:2023-02-20
通讯作者: 刘素蕊,女,主要研究血液成分临床应用,（E-mail）lsr1029@126.com。
作者简介:王巧改（1981-）,女,主要从事血液免疫细胞因子研究,（E-mail）atc507@126.com。
基金资助:
*本课题受河北省卫生健康委医学科学研究基金（No.20211270）、河北省中医药管理局科研基金（No.2020261）资助

Platelet Rich Fibrin Releasate Improves Atopic Dermatitis in Rats: an Experimental Study

WANG Qiao-gai, BI Li-bo, LIU Su-rui

Department of Clinical Laboratory, No. 980 Hospital, Joint Logistics Support Force, Shijiazhuang 050082

Received:2022-09-16 Published:2023-02-20

摘要/Abstract

摘要： 目的观察富血小板纤维蛋白上清（platelet rich fibrin-releasate,PRF-r）对2,4-二硝基氯苯（DNCB）诱导的大鼠特应性皮炎（atopic dermatitis,AD）的影响,并试图探索其可能的潜在机制。方法 SD大鼠42只（含制备PRF-r所需的大鼠10只）随机分为正常对照组、模型组（DNCB）、糠酸莫米松乳膏组和PRF-r组4组,每组8只。抽取正常大鼠不抗凝尾静脉血制备PRF-r。通过反复局部应用DNCB在大鼠背部诱导AD。PRF-r组和糠酸莫米松乳膏组从造膜第7天开始连续涂抹10天。对AD部位进行临床皮肤评分。AD组织用苏木精-伊红（H&E）染色以观察皮肤病理学改变。采用酶联免疫吸附实验（ELISA）测定大鼠血清中白介素4（IL-4）和γ-干扰素（IFN-γ）水平。免疫组化检测AD部位IL-4和IFN-γ的表达。结果 PRF-r显著减轻DNCB诱导的大鼠AD样症状,包括皮炎严重程度,降低了皮炎评分（PRF-r组：1.8±0.27,DNCB模型组：4.9±0.44）。进一步实验发现,与正常对照组大鼠血清IFN-γ （140.43±8.21） pg/mL和IL-4 （206.63±2.17） pg/mL水平相比,DNCB诱导的模型组大鼠血清IFN-γ水平明显减少（114.21±6.67） pg/mL,IL-4水平增加（221.71±2.58） pg/mL。糠酸莫米松乳膏和PRF-r治疗后IFN-γ表达提升[（127.83±7.59） pg/mL,（145.66±7.92） pg/mL],IL-4水平回落[（210.45±4.15） pg/mL,（212.26±3.71） pg/mL],趋于正常组水平。免疫组化显示各组AD局部组织中IFN-γ和IL-4的表达与血清中趋势一致。结论 PRF-r可能通过调节Th2型炎症反应和Th1/2细胞因子水平来改善DNCB诱导的大鼠AD。

关键词: 富血小板纤维蛋白上清, 特应性皮炎, 细胞因子, 免疫

Abstract: Objective This study was aimed to investigate the effect of PRF-r on the 2,4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis (AD) rat model and explore its possible underlying mechanisms. Methods SD rats (including 10 rats required for PRF-r preparation) were divided into four groups: untreated rats (control group), AD-induced rats (DNCB group), AD-induced rats treated with Mometasone furoate (Mometasone furoate group) and AD-induced rats treated with PRF-r (PRF-r group), with 8 in each group. Non-anticoagulant caudal vein blood of normal rats was extracted to prepare PRF-r. AD was induced in the back of rats by repeated topical administration of DNCB. PRF-r and mometasone furoate groups were treated for 10 consecutive days from the 7th day. Clinical skin scoring was performed on the AD site. AD tissues were stained with hematoxylin eosin (H&E) to observe the pathological changes. Levels of interleukin-4 (IL-4) and γ-Interferon (IFN-γ) were determined by enzyme-linked immunosorbent assay (ELISA) in the sera of rats and by immunohistochemistry at the AD site. Results PRF-r significantly improved the AD-like symptoms, and decreased the dermatitis score compared with the DNCB induced model group(PRF-r group:1.8±0.27, DNCB induced model group: 4.9±0.44). ELISA analysis showed that compared to the serum levels of IFN-γ (140.43±8.21) pg/mL and IL-4 (206.63±2.17) pg/mL in the control group, IFN-γ in the DNCB induced model group was significantly reduced (114.21± 6.67) pg/mL, while IL-4 was increased markedly (221.71±2.58) pg/mL. Meanwhile, Mometasone furoate cream and PRF-r increased the serum level of IFN-γ (127.83±7.59) pg/mL, (145.66±7.92) pg/mL and brought IL-4 back to the normal level (210.45±4.15) pg/mL, (212.26±3.71) pg/mL respectively. Further immunohistochemical testing showed the levels of IFN-γ and IL-4 in each group tended to be consistent with those in the sera. Conclusion PRF-r may inhibit DNCB induced AD in rats by regulating Th2 type inflammatory response and Th1/2 cytokine level.

Key words: Platelet rich fibrin-releasate, Atopic dermatitis, Cytokine, Immune

中图分类号:

R392

王巧改, 毕利博, 刘素蕊. 富血小板纤维蛋白上清改善大鼠特应性皮炎的实验研究^*[J]. 临床输血与检验, 2023, 25(1): 69-74.

WANG Qiao-gai, BI Li-bo, LIU Su-rui. Platelet Rich Fibrin Releasate Improves Atopic Dermatitis in Rats: an Experimental Study[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2023, 25(1): 69-74.

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富血小板纤维蛋白上清改善大鼠特应性皮炎的实验研究^*

Platelet Rich Fibrin Releasate Improves Atopic Dermatitis in Rats: an Experimental Study

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