山东省ABO亚型等位基因的分析研究*

doi:10.3969/j.issn.1671-2587.2024.04.009

临床输血与检验 ›› 2024, Vol. 26 ›› Issue (4): 479-483.DOI: 10.3969/j.issn.1671-2587.2024.04.009

山东省ABO亚型等位基因的分析研究^*

冯智慧¹, 卜祥茂², 浑守永³, 于淑红⁴, 庄文华⁵, 宿军⁶, 张韦⁷, 耿微⁸

¹青岛市中心血站输血研究部,山东青岛;
²青岛市妇女儿童医院检验科,山东青岛;
³山东省立医院输血科,山东济南;
⁴烟台毓璜顶医院输血科,山东烟台;
⁵日照市中心血站血液免疫科,山东日照;
⁶潍坊市中心血站输血研究所,山东潍坊;
⁷滨州市中心血站质量管理科,山东滨州;
⁸济宁市中心血站血液供应部,山东济宁

收稿日期:2024-05-27 出版日期:2024-08-20 发布日期:2024-09-23
作者简介:冯智慧,主要从事血液免疫学、临床输血研究,（E-mail）qd_hla@163.com。
基金资助:
*本课题受山东省输血协会科研基金资助项目（No.2024-sdsx03）资助

Analysis on Alleles of ABO Subgroups in Shandong Province

FENG Zhihui¹, BU Xiangmao², HUN Shouyong³, YU Shuhong⁴, ZHUANG Wenhua⁵, SU Jun⁶, ZHANG Wei⁷, GENG Wei⁸

¹Qingdao Blood Center, Institute of Transfusion Medicine, Qingdao;
²Qingdao Women and Children's Hospital, Clinical Laboratory, Qingdao;
³Shandong Provincial Hospital, Blood Transfusion Department, Jinan;
⁴Yantai Yu Huang Ding Hospital, Blood Transfusion Department, Yantai;
⁵Rizhao Blood Center, Hematology and Immunology Department, Rizhao;
⁶Weifang Blood Center, Institute of Transfusion Medicine, Weifang;
⁷Binzhou Blood Center, Quality Management Department, Binzhou;
⁸Jining Blood Center, Blood Distribution Department, Jining

Received:2024-05-27 Online:2024-08-20 Published:2024-09-23

摘要/Abstract

摘要： 目的对山东省内医疗机构及血站送检的疑似ABO亚型的标本进行血清学、ABO基因测序及单倍型序列测定,分析山东省人群ABO亚型等位基因的分布和分子机制。方法血清学检测疑似为ABO亚型的无偿献血者标本239例及临床就诊患者标本701例,共940例采用PCR-SBT方法进行ABO基因序列分析;采用单克隆测序方法检测单体型;软件分析比对测序结果,确定该标本的等位基因型。结果分子测序发现山东省A基因亚型等位基因12种,以ABO*A2.05最为常见;B基因亚型等位基因15种,以ABO*BW.03最为常见;复合型ABO糖基转移酶基因6种,以ABO*BA.04最为常见;嵌合体血型6例。结论山东省B亚型的数量和种类均多于A亚型;A亚型以ABO*A2.05和ABO*A3.07最为常见;B亚型以ABO*BW.03和ABO*BW.27多见;ABO*BA.04和ABO*cisAB.01等位基因是最常见的复合型ABO糖基转移酶等位基因类型。

关键词: ABO亚型, A亚型, B亚型, 复合型ABO糖基转移酶基因, 测序

Abstract: Objectives Serology, ABO gene sequencing, and haplotype sequencing were performed on samples sent to our laboratory by medical institutions and blood stations in Shandong Province for suspected ABO subgroups. The distribution and molecular mechanism of ABO subgroup alleles in the population of Shandong Province were elucidated. Methods 239 blood donors suspected to be ABO subtypes and 701 clinical patients were tested for ABO gene sequence using PCR SBT method; Using monoclonal sequencing method to detect haplotypes; The software analyzes and compares the sequencing results to determine the allele subgroup of the sample. Results Molecular sequencing revealed 12 alleles of the A gene subgroup in Shandong Province, with ABO*A2.05 being the most common; There are 15 alleles in the B gene subgroup, with ABO*BW.03 being the most common; There are 6 types of complex ABO glycosyltransferase genes, with ABO*BA.04 being the most common; 6 cases of chimeric blood type. Conclusion ABO*A2.05 and ABO*A3.07 are the most common subgroups of the A gene in Shandong Province; ABO*BW.03 and ABO*BW.27 are the most common subgroups of the B gene; The ABO*BA.04 and ABO*cisAB.01 alleles are common complex ABO glycosyltransferase alleles.

Key words: ABO subgroups, A subgroups, B subgroups, ABO Glycosyltransferase with Dual Specificity Sequencing

中图分类号:

R457

冯智慧, 卜祥茂, 浑守永, 于淑红, 庄文华, 宿军, 张韦, 耿微. 山东省ABO亚型等位基因的分析研究^*[J]. 临床输血与检验, 2024, 26(4): 479-483.

FENG Zhihui, BU Xiangmao, HUN Shouyong, YU Shuhong, ZHUANG Wenhua, SU Jun, ZHANG Wei, GENG Wei. Analysis on Alleles of ABO Subgroups in Shandong Province[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2024, 26(4): 479-483.

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山东省ABO亚型等位基因的分析研究^*

Analysis on Alleles of ABO Subgroups in Shandong Province

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