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临床输血与检验

临床输血与检验 ›› 2025, Vol. 27 ›› Issue (2): 200-206.DOI: 10.3969/j.issn.1671-2587.2025.02.010

• 临床输血 • 上一篇    下一篇

血小板中lncRNA在结肠癌早期筛查中的应用价值

李秀丽, 车舒平, 曹荣祎   

  1. 哈尔滨医科大学附属第一医院输血科,黑龙江哈尔滨 150001
  • 收稿日期:2024-11-28 出版日期:2025-04-20 发布日期:2025-04-17
  • 通讯作者: 曹荣祎,主要从事输血医学研究,（E-mail）crywangye@126.com。
  • 作者简介:李秀丽,主要从事输血医学研究,（E-mail）lixiuli1208@126.com。

Platelet lncRNA in the Early Screening of Colon Cancer

LI Xiuli, CHE Shuping, CAO Rongyi   

  1. Department of Blood Transfusion, The First Affiliated Hospital of Harbin Medical University, Harbin 150001
  • Received:2024-11-28 Online:2025-04-20 Published:2025-04-17

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摘要： 目的 分析血小板长链非编码RNA（long noncoding RNA, lncRNA）在结肠癌（colon cancer, CC）早期筛查中的诊断价值。方法 运用R语言中“limma”包对包含CC患者和健康对照者血小板lncRNA数据的GSE68086和GSE183635数据集做差异分析,取交集筛选出差异表达lncRNA（differentially expressed lncRNA, DElncRNA）。使用R语言中Wilcoxon rank sum tes统计学方法分析DElncRNA在CC组织与正常对照组织中的的差异表达。实时荧光定量PCR（quantitative real-time PCR, qRT-PCR）检测60名健康对照者和66名CC患者中血小板LINC00926的表达水平,研究采用了受试者工作特征（receiver operator characteristic, ROC）评判诊断效能。结果 GSE68086数据集差异分析得到341个DElncRNA,GSE183635数据集差异分析得到21个DElncRNA,取交集后获得关键DElncRNA LINC00926。R语言Wilcoxon rank sum test统计学方法分析显示,血小板LINC00926在CC组织中的表达水平低于正常结肠组织（P<0.05）。qRT-PCR验证了血小板LINC00926在CC患者和早期CC患者中下调（P均<0.001）。血小板LINC00926对CC诊断的曲线下面积（area under the curve, AUC）为0.781,特异度为80.0%,敏感度为74.2%;对早期CC诊断的AUC为0.761,特异度为78.3%,敏感度为70.8%;血小板LINC00926与癌胚抗原（carcinoembryonic antigen, CEA）构成的联合诊断模型对CC诊断的AUC为0.864,特异度为91.7%,敏感度为69.7%;对早期CC的AUC为0.851,特异度为75.0%,敏感度为85.4%。（P<0.05）。在CC及早期CC中,血小板LINC00926联合CEA对AUC的检测优于单独检测（z分别=-2.619,2.388,P均<0.05）。结论 结肠癌患者血小板中LINC00926表达下调,血小板LINC00926可能提高结肠癌早期筛查的诊断价值。

关键词: 结肠癌, 血小板中lncRNA, 早期筛查

Abstract: Objective To analyze the diagnostic value of long noncoding RNAs (lncRNAs) in platelets in early screening of colon cancer (CC). Methods The GSE68086 and GSE183635 datasets, which contain platelet lncRNAs data from CC patients and healthy controls, were analyzed using the “limma” package in R. The intersection of the results was utilized to identify differentially expressed lncRNAs (DElncRNAs). The Wilcoxon rank sum test in R language was used to analyze the differential expression of DElncRNAs in CC tissues and normal control tissues. Quantitative real-time PCR (qRT-PCR) was used to detect the expression levels of platelet LINC00926 in 60 healthy controls and 66 CC patients. The receiver operating characteristic (ROC) was used to evaluate diagnostic performance. Results The differential analysis of GSE68086 dataset identified 341 DElncRNAs, and the GSE183635 dataset identified 21 DElncRNAs. The key DElncRNA LINC00926 was obtained from the intersection. R language Wilcoxon rank sum test showed that the expression level of platelet LINC00926 in CC tissues was lower than that in normal colon tissues (P<0.05). qRT-PCR confirmed that down-regulation of platelet LINC00926 in CC patients and early-stage CC patients (both P<0.001). The area under the curve (AUC) of platelet LINC00926 for CC diagnosis was 0.781, with a specificity of 80.0% and sensitivity of 74.2%. AUC for early-stage CC diagnosis was 0.761, with a specificity of 78.3% and sensitivity of 70.8%. The combined diagnostic model of platelet LINC00926 and carcinoembryonic antigen (CEA) had an AUC of 0.864, a specificity of 91.7% and sensitivity of 69.7%. The AUC for early-stage CC diagnosis was 0.851, with a specificity of 75.0% and sensitivity of 85.4% (P<0.05). In both CC and early-stage CC, the detection of AUC of platelet LINC00926 combined with CEA was superior to that of single detection (z =-2.619, 2.388, respectively, both P<0.05). Conclusion The LINC00926 expression in platelets of CC patients was down-regulated. It suggests that platelet LINC00926 may enhance the diagnostic value of early screening for colon cancer.

Key words: Colon cancer, Platelet lncRNA, Early screening

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