• 中国科学论文统计源期刊
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  • 日本科学技术振兴机构数据库(JST)
临床输血与检验

临床输血与检验 ›› 2024, Vol. 26 ›› Issue (1): 29-34.DOI: 10.3969/j.issn.1671-2587.2024.01.005

• 基础研究 • 上一篇    下一篇

不同核黄素浓度与可见光光照强度对血浆中大肠杆菌的影响*

刘鸿, 莫琴, 马荣钠, 贾尧, 伍晓菲, 王迅   

  1. 上海市血液中心,上海 200051
  • 收稿日期:2023-11-20 发布日期:2024-03-13
  • 通讯作者: 王迅,主要从事输血传染病、血液筛查、质量控制与评估方面研究,（E-mail）wangxun@sbc.org.cn。
  • 作者简介:刘鸿,主要从事病原体灭活、输血传染病方面研究,（E-mail）liuhong@sbc.org.cn。
  • 基金资助:
    *本课题受中国输血协会威高科研基金面上项目（No.02J2202-06）资助

The Effects of Riboflavin Concentrations and Visible Light Intensities on E. Coli in Plasma

LIU Hong, MO Qin, MA Rongna, JIA Yao, WU Xiaofei, WANG Xun   

  1. Shanghai Blood Center, Shanghai 200051
  • Received:2023-11-20 Published:2024-03-13

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摘要： 目的 研究不同核黄素浓度与可见光光照强度对血浆中大肠杆菌的影响,为建立血浆中污染细菌的灭活方法提供备选条件。方法 随机选取谷氨酸氨基转移酶（ALT）检测不合格的新鲜冰冻血浆,按照处理方式将实验分为三大类：第一类为只添加菌液但不做任何处理的对照组,第二类为只添加菌液并接受光照处理的光照组,第三类为添加菌液与核黄素并接受光照处理的实验组。其中实验组根据核黄素浓度又细分为 50、100、150和300 µM的核黄素浓度组。光照组和各核黄素浓度组分别接受420 nm可见光的光照处理,每轮光照的光照强度分别为50 mW/cm2、75 mW/cm2和100 mW/cm2,光照时间为55 min（分别在25、35、45和55 min取样检测）,光照结束后通过细菌培养评估不同核黄素浓度与可见光光照强度对血浆中大肠杆菌的影响。结果 在420 nm光照55 min后,各核黄素浓度下大肠杆菌浓度分别下降了1.7~3.5 log（50 mW/cm2）,2.8~≥4.4 log（75 mW/cm2）,4.0~≥4.7 log（100 mW/cm2）。其中,高强度的光照（100 mW/cm2）协同高浓度的核黄素（150 µM和300 µM）能够有效降低血浆中大肠杆菌的浓度达到4 log以上。而光照强度的增加与核黄素浓度升高也会增加对大肠杆菌的处理效果,两者与大肠杆菌处理效果之间也呈正相关。结论 在420 nm波长、光照强度75 mW/cm2、核黄素浓度为150 µM和300 µM时,核黄素可见光照射方法对血浆中大肠杆菌具有最理想的处理效果,可有效降低血浆中污染的大肠杆菌浓度。

关键词: 病原体灭活, 核黄素, 大肠杆菌, 细菌污染, 血液安全

Abstract: Objective To evaluate the effects of varying riboflavin concentrations and visible light intensities on E. coli in plasma, based on which to propose alternative methods for reducing bacterial contamination in plasma. Methods Fresh frozen plasma that failed the glutamate aminotran sferase (ALT) test was randomly selected and divided into control, light, and experimental groups after being differently treated. Plasma mixed with E. coli was added to each group. The control group did not undergo any treatment, the light group was subjected to irradiation without addition of riboflavin, and the experimental group received irradiation with addition of riboflavin at a working concentration of 50~300 µM. The light and experimental groups were placed in a pathogen reduction device and irradiated with 420 nm visible light with 50 mW/cm2, 75 mW/cm2, and 100 mW/cm2, respectively. The duration of the light treatment was 55 minutes. The study evaluated the effects of different riboflavin concentrations and visible light intensities on E. coli in plasma. Bacterial culture was performed at the end of light exposure to assess the outcomes. Results After treatment with 420 nm visible light for 55 minutes, the concentration of E. coli decreased by 1.7~3.5 log (50 mW/cm2),2.8~≥4.4 log (75 mW/cm2),and 4.0~≥4.7 log (100 mW/cm2) respectively. Treatment with high-intensity light (100 mW/cm2) and high riboflavin concentration (150 and 300 µM) reduced over 4 logs of E. coli in plasma. Increased light intensity and riboflavin concentration both enhanced the reduction effect on E. coli. The two factors were also found to be in positive correlation with E. coli reduction efficacy. Conclusion At the wavelength of 420 nm, light intensity of 75 mW/cm2, riboflavin concentrations of 150 µM and 300 µM, riboflavin visible light irradiation method has the best treatment effect on E. coli in plasma, and could effectively reduce the concentration of E. coli contaminated in plasma.

Key words: Pathogen reduction, Riboflavin, E. coli, Bacterial contamination, Blood safety

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