HPL-CS温敏凝胶促高原糖尿病大鼠难愈性创面愈合的研究*

doi:10.3969/j.issn.1671-2587.2025.04.001

临床输血与检验 ›› 2025, Vol. 27 ›› Issue (4): 433-441.DOI: 10.3969/j.issn.1671-2587.2025.04.001

• 基础研究 • 下一篇

HPL-CS温敏凝胶促高原糖尿病大鼠难愈性创面愈合的研究^*

王辉¹, 赵琴¹, 陈立力², 黄永莉², 张进进², 邢颜超²

¹新疆医科大学研究生院,新疆乌鲁木齐 830054;
²新疆军区总医院输血科,新疆乌鲁木齐 830099

收稿日期:2024-12-25 发布日期:2025-08-22
通讯作者: 邢颜超,主要从事临床输血、创面修复、细胞治疗的基础及临床研究,（E-mail）xingyanchao@aliyun.com。共同通信作者：张进进,主要从事临床输血与细胞治疗研究工作,（E-mail）13319805828@qq.com。
作者简介:王辉,主要从事临床输血与细胞治疗研究工作,（E-mail）wanghui9908@163.com。并列第一作者：赵琴,主要从事临床输血与创面修复研究工作,（E-mail）zhaoqin_@outlook.com。
基金资助:
*本课题受新疆维吾尔自治区自然科学基金项目（No.2022D01C342）资助

HPL-CS Thermosensitive Hydrogel for Promoting Refractory Wound Healing in Diabetic Rat at High Altitude

WANG Hui¹, ZHAO Qin¹, CHEN Lili², HUANG Yongli², ZHANG Jinjin², XING Yanchao²

¹Graduate School of Xinjiang Medical University, Urumqi 830054;
²Department of Transfusion Medicine, General Hospital of Xinjiang Military Command, Urumqi 830000

Received:2024-12-25 Published:2025-08-22

摘要/Abstract

摘要： 目的本研究旨在探讨负载人血小板裂解液（HPL）的壳聚糖（CS）温敏凝胶的制备,并在高原糖尿病大鼠难愈性创面中验证其修复作用。方法调整CS和HPL的比例,通过表观和电镜观察确定HPL-CS温敏凝胶的最佳制备条件,并在不同温度下观察凝胶形成情况。将SD大鼠均分为平原组和高原组后,再随机分为对照组、模型组、CS组和HPL-CS组。对照组无处理,模型组仅患糖尿病,CS组用CS凝胶每日涂抹创面,HPL-CS组用HPL-CS温敏凝胶每日涂抹创面。比较第0、3、7、14、21天各组的创面愈合情况,并在第7、14、21天取材做苏木精-伊红染色（H&E）和Masson染色。结果 HPL:CS=1:1是制备HPL-CS凝胶的最佳比例;HPL-CS温敏凝胶在37 ℃形成凝胶,恢复至22 ℃时液体析出,但不能完全恢复液体状态;无论平原组还是高原组,HPL-CS组创面愈合率在各个时间点都高于模型组和CS组;病理显示,HPL-CS组相较于其他组炎症细胞减少、上皮细胞增生、胶原纤维排列紧密、出现大量新生血管。结论 HPL-CS温敏凝胶显著提升了高原环境下糖尿病难愈性创面的修复效果。

关键词: HPL-CS凝胶, 糖尿病难愈性创面, 高原环境, 温敏性, 生长因子

Abstract: Objective To investigate the preparation of chitosan (CS) temperature-sensitive gel loaded with human platelet lysate (HPL), and to verify its repairing effect in refractory wounds in high-altitude diabetic rats. Methods The ratio of CS and HPL was adjusted, and the optimal preparation conditions of HPL-CS temperature-sensitive gel were determined by apparent and electron microscopy observation, and the gel formation was observed at different temperatures. SD rats were divided into plain group and plateau group, and then randomly divided into control group, model group, CS group and HPL-CS group. There was no treatment in the control group, only modeling was done in the diabetic group, CS gel was applied daily in the CS group, and HPL-CS temperature sensitive gel was applied daily in the HPL-CS group. The wound healing of each group was compared at day 0, 3, 7, 14 and 21, and samples were collected at day 7, 14 and 21 for Hematoxylin-Eosin staining (H&E)and Masson staining. Results HPL:CS=1:1 was the optimal ratio to prepare of HPL-CS gel. HPL-CS temperature-sensitive gel formed a gel at 37 ℃, and the liquid precipitated at 22 ℃, but the liquid state could not be completely restored. The wound healing rate in HPL-CS group was higher than that in Model group and in the CS group at all time points, no matter in plain group or plateau group. Pathological results showed that the HPL-CS group had fewer inflammatory cells, more epithelial cells, more tightly arranged collagen fibers, and much more neovascularization compared with the other groups. Conclusion HPL-CS temperature-sensitive gel can significantly improve the repair effect of diabetes refractory wounds in high-altitude environment.

Key words: HPL-CS hydrogel, Diabetic refractory wounds, High-altitude environment, Thermosensitivity, Growth factor

中图分类号:

R641

王辉, 赵琴, 陈立力, 黄永莉, 张进进, 邢颜超. HPL-CS温敏凝胶促高原糖尿病大鼠难愈性创面愈合的研究^*[J]. 临床输血与检验, 2025, 27(4): 433-441.

WANG Hui, ZHAO Qin, CHEN Lili, HUANG Yongli, ZHANG Jinjin, XING Yanchao. HPL-CS Thermosensitive Hydrogel for Promoting Refractory Wound Healing in Diabetic Rat at High Altitude[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2025, 27(4): 433-441.

参考文献

[1] EMING S A,MURRAY P J,PEARCE E J.Metabolic orchestration of the wound healing response[J]. Cell Metab,2021,33(9):1726-1743.
[2] SHEN C M,QI Y Z.Granulation-embedded autologous skin grafting improves healing chronic wounds at high altitudes:a pilot study[J]. Wound Manag Prev,2024, 70(2). DOI: 10.25270/wmp.23028.
[3] SEN C K.Human wound and its burden:updated 2022 compendium of estimates[J]. Adv Wound Care (New Rochelle),2023,12(12):657-670.
[4] FENG S X,PENG X,DENG Y Q,et al.Biomimetic nanozyme-decorated smart hydrogel for promoting chronic refractory wound healing[J]. ACS Appl Mater Interfaces,2024,16(44):59862-59879.
[5] ARIF U,HAIDER S,HAIDER A,et al.Biocompatible polymers and their potential biomedical applications:a review[J]. Curr Pharm Des,2019,25(34):3608-3619.
[6] ABD EL-HACK M E,EL-SAADONY M T,SHAFI M E, et al. Antimicrobial and antioxidant properties of chitosan and its derivatives and their applications: a review[J]. Int J Biol Macromol,2020,164:2726-2744.
[7] LIU S H,WANG J N,WANG X,et al.Smart chitosan-based nanofibers for real-time monitoring and promotion of wound healing[J]. Int J Biol Macromol,2024,282(Pt 1):136670.
[8] RAHMANIAN-DEVIN P,BARADARAN RAHIMI V, ASKARI V R.Thermosensitive chitosan-β-glycerophosphate hydrogels as targeted drug delivery systems: an overview on preparation and their applications[J]. Adv Pharmacol Pharm Sci,2021,2021: 6640893.
[9] LASCHKE M W,MENGER M D.The simpler,the better:tissue vascularization using the body's own resources[J]. Trends Biotechnol,2022,40(3):281-290.
[10] JIN T T,FU Z X,ZHOU L Y,et al.GelMA loaded with platelet lysate promotes skin regeneration and angiogenesis in pressure ulcers by activating STAT3[J]. Sci Rep,2024,14(1):18345.
[11] BARDAA S,MAKNI K,BOUDAOUARA O,et al.Development and evaluation of the wound healing effect of a novel topical cream formula based on Ginkgo biloba extract on wounds in diabetic rats[J]. Biomed Res Int,2021,2021:6474706.
[12] 何斯,严辰媛,刚乔健,等. 高原地区难愈合创面病理变化和特点[J]. 医学综述,2022,28(14):2746-2750.
[13] 祁永章,陈亚莉,王滋兴,等. 红景天苷在高海拔地区创伤性慢性创面愈合中的作用及其研究[J]. 中国美容医学, 2022,31(3):96-99.
[14] HUANG P P,SU W,HAN R,et al.Physicochemical, antibacterial properties,and compatibility of ZnO-NP/chitosan/β-glycerophosphate composite hydrogels[J]. J Microbiol Biotechnol,2022, 32(4):522-530.
[15] ZHU Y F,LIU H F,WU P,et al.Multifunctional injectable hydrogel system as a mild photothermal-assisted therapeutic platform for programmed regulation of inflammation and osteo-microenvironment for enhanced healing of diabetic bone defects in situ[J]. Theranostics, 2024,14(18):7140-7198.
[16] YUAN M,LIU K,JIANG T,et al.GelMA/PEGDA microneedles patch loaded with HUVECs-derived exosomes and Tazarotene promote diabetic wound healing[J]. J Nanobiotechnology,2022,20(1):147.
[17] 王淑君,李兆杰,杨奕,等. 血小板衍生制品促进创面愈合的研究进展[J]. 临床输血与检验,2024,26(6):825-834.
[18] 冯慧慧,林丹,俞露,等. 血小板裂解物体外抑菌活性及其与抗生素的协同效应研究[J]. 临床输血与检验, 2024, 26(4):446-450.
[19] YOUSEFI-AHMADIPOUR A,EBRAHIMI-BAROUGH S,NIKNIA S,et al.Therapeutic effects of combination of platelet lysate and sulfasalazine administration in TNBS-induced colitis in rat[J]. Biomed Pharmacother, 2020,125:109949.

HPL-CS温敏凝胶促高原糖尿病大鼠难愈性创面愈合的研究^*

HPL-CS Thermosensitive Hydrogel for Promoting Refractory Wound Healing in Diabetic Rat at High Altitude

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