富血小板纤维蛋白对宫腔粘连创面修复的影响研究*

doi:10.3969/j.issn.1671-2587.2022.03.018

临床输血与检验 ›› 2022, Vol. 24 ›› Issue (3): 363-368.DOI: 10.3969/j.issn.1671-2587.2022.03.018

富血小板纤维蛋白对宫腔粘连创面修复的影响研究^*

杨慕坤, 王子君, 崔广霞, 陈麟凤, 白文佩

100038 首都医科大学附属北京世纪坛医院妇产科（杨慕坤,王子君,崔广霞,白文佩）; 首都医科大学附属北京世纪坛医院输血科（陈麟凤）

收稿日期:2022-04-29 出版日期:2022-06-20 发布日期:2022-06-22
通讯作者: 白文佩,女,主任医师,博士生导师,主要从事妇科内分泌与妇科肿瘤方面研究,（E-mail）Baiwp@bjsjth.cn。
作者简介:杨慕坤（1987-）,女,湖北武汉人,主治医师,博士,主要从事妇科内分泌相关研究,（E-mail）yangmukun@gmail.com。
基金资助:
*本课题受北京市医院管理中心“登峰”计划专项经费资助（No.DFL20190701）; 首都医科大学附属北京世纪坛医院院青年基金（No.2021-q21）; 首都医科大学附属北京世纪坛医院院基金（No.2019-C11）资助

Effect of Platelet-rich Fibrin on Wound Repair of Intrauterine Adhesions

YANG Mu-kun, WANG Zi-jun, CUI Guang-xia, et al

Department of Obstetrics and Gynecology,Beijing Shijitan Hospital,Capital Medical University 100038

Received:2022-04-29 Online:2022-06-20 Published:2022-06-22

摘要/Abstract

摘要： 目的评价富血小板纤维蛋白（platelet-rich fibrin,PRF）对宫腔粘连创面修复的形态学影响。方法将经宫腔镜证实为宫腔粘连的患者68例按1∶1随机分为PRF组与对照组,均行宫腔镜宫腔粘连分离术（TCRA）并留取子宫内膜组织,PRF组在TCRA后即刻、4周后宫腔置入PRF,对照组宫腔内未置入药物。8周后两组均行宫腔镜二探术评估粘连情况并再次留取子宫内膜组织。评估治疗前与治疗后8周子宫内膜形态学变化。结果形态学研究显示PRF组治疗后腺体数量、Ki67、细胞角蛋白18（Cytokeratin 18,CK-18）、波形蛋白（Vimentin）平均荧光强度均增加,纤维化面积比例减小。PRF组子宫内膜腺体数量、Ki67平均荧光强度均显著高于对照组（P<0.05）;纤维化面积比例小于对照组（P<0.01）。结论应用PRF治疗宫腔粘连,可以显著促进创面修复。

关键词: 宫腔粘连, 富血小板纤维蛋白, 生长因子, 创面修复

Abstract: Objective To evaluate the morphological effects of Platelet-rich fibrin （PRF） on wound repair of intrauterine adhesion. Methods 68 patients with intrauterine adhesions were randomly divided into PRF group and control group by 1∶1. All patients received TCRA and took endometrial tissue. PRF was injected into intrauterine immediately and 4 weeks after TCRA in PRF group,while no drug was used in control group. 8 weeks later,the two groups underwent second hysteroscopy. Endometrial morphologic changes were evaluated 8 weeks after treatment. Results In PRF group,the number of endometrial glands,Ki67,Cytokeratin 18（CK-18）,Vimentin average fluorescence intensity increased after treatment,and the proportion of fibrosis area decreased. The number of endometrial glands and average fluorescence intensity of Ki67 in PRF group were significantly higher than those in control group（P<0.05）. The proportion of fibrotic area was smaller than that of control group（P<0.01）. Conclusion PRF treatment can significantly promote wound repair after intrauterine operation.

Key words: Intrauterine adhesions, Platelet-rich fibrin, Growth factor, Wound repair

中图分类号:

R711.74

杨慕坤, 王子君, 崔广霞, 陈麟凤, 白文佩. 富血小板纤维蛋白对宫腔粘连创面修复的影响研究^*[J]. 临床输血与检验, 2022, 24(3): 363-368.

YANG Mu-kun, WANG Zi-jun, CUI Guang-xia, et al. Effect of Platelet-rich Fibrin on Wound Repair of Intrauterine Adhesions[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2022, 24(3): 363-368.

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富血小板纤维蛋白对宫腔粘连创面修复的影响研究^*

Effect of Platelet-rich Fibrin on Wound Repair of Intrauterine Adhesions

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