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临床输血与检验

临床输血与检验 ›› 2016, Vol. 18 ›› Issue (5): 461-464.DOI: 10.3969/j.issn.1671-2587.2016.05.018

• 临床输血研究 • 上一篇    下一篇

无偿献血者HCV RNA与抗-HCV及ALT检测结果的相关性

郭燕, 叶世辉, 蔡斌, 景媛媛, 巩晗实, 杨莹, 黄蕾, 段勇   

  1. 710061 西安,陕西省血液中心
  • 收稿日期:2016-03-13 出版日期:2016-10-20 发布日期:2017-05-19
  • 通讯作者: 段勇（1972-）,男,副主任技师,（E-mail）13891859143@139.com。
  • 作者简介:郭燕（1980-）,女,陕西西安人,主管技师,博士,主要从事血源性疾病的相关研究,（Tel）18691876093（E-mail）20524988@qq.com。

Relationship between anti-HCV,HCV RNA and ALT in Volunteer Blood Donors

GUO Yan, YE Shi-hui, CAI Bin, et al   

  1. Shanxi Blood Center,Xi'an 710061
  • Received:2016-03-13 Online:2016-10-20 Published:2017-05-19

PDF

732

被引次数

10

摘要： 目的 讨论无偿献血人群丙型肝炎病毒抗体（抗-HCV）、HCV RNA、丙氨酸氨基转移酶（ALT）检测结果之间的相关性。方法 采用ELISA法检测抗-HCV阳性标本235份,荧光定量PCR法检测病毒RNA,速率法测定ALT,并对抗-HCV（+）/HCV RNA（-）标本进行追踪回访。结果 235份抗-HCV阳性标本中,HCV RNA阳性140例（阳性率59.57%）;抗-HCV S/CO值1~5实验组和5.01~9.99实验组,HCV RNA阳性率分别为34.29%、26.47%;S/CO≥10时,HCV RNA阳性率为81.68%,与其他2组的差异有统计学意义（χ2=45.15,P<0.05;χ2=39.41,P<0.05）。抗-HCV（+）/HCV RNA（+）与抗-HCV（+）/HCV RNA（-）组的ALT异常率分别为2.86%、1.05%,两组的差异无统计学意义（χ2=0.88,P>0.05）。抗-HCV（+）/HCV RNA（+）与抗-HCV（+）/HCV RNA（-）组献血者的年龄分别是38.05±11.35岁和33.81±11.50岁,两组的差异有统计学意义（t=2.79,P<0.05）。95份抗-HCV（+）/HCV RNA（-）标本成功回访23例,其中1份标本回访检测抗-HCV转阴。结论 HCV RNA阳性率与抗-HCV的S/CO值有一定相关性,抗-HCV阳性的无偿献血人群中,ALT异常率与HCV RNA无相关性,感染者年龄与HCV RNA有一定相关性。

关键词: 无偿献血者, 抗- HCV, HCV RNA

Abstract: Objective To investigate the relationship between anti-HCV,HCV RNA and ALT in volunteer blood donors. Methods Fluorescent quantitative PCR was used to detect viral loads and kinetic method was employed to assay ALT levels in 235 anti-HCV positive samples who were tested by ELISA. A six month follow up was performed to monitor the anti-HCV（+）/HCV RNA（-） samples. Results Among 235 anti-HCV positive samples,140（59.57%） were positive for HCV RNA. The HCV RNA positive rate in groups of S/CO ratio 1~5 and 5.01~9.99 were 34.29% and 26.47%, respectively. The HCV RNA positive rate in group of S/CO ratio≥10 was 81.68% and statistically significant differences were found between this and the other two groups（χ2 =45.15,P<0.05;χ2=39.41,P<0.05）. The ALT abnormality rates of anti-HCV（+）/HCV RNA（+） and anti-HCV（+）/HCV RNA（-） groups were 2.86% and 1.05%, respectively. No statistically significant difference was found between them（χ2 =0.88,P≥0.05） . The age of donors with anti-HCV（+）/HCV RNA（+）were older than those with the anti-HCV（+）/HCV RNA（-) （χ2 =7.81,P<0.05）. Among 95 blood donors,23 received retrospective survey and the anti-HCV results of one sample was negative in the second detection. Conclusion The positive rate of HCV RNA has relationship with the S/CO ratio of anti-HCV and the age of HCV infected blood donors. No correlation was found between the unqualified rate of ALT and HCV RNA in anti-HCV positive volunteer blood donors.

Key words: Volunteer blood donors, Anti-HCV, HCV RNA

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