合肥地区无偿献血人群CD36抗原缺失的调查*

doi:10.3969/j.issn.1671-2587.2021.02.004

临床输血与检验 ›› 2021, Vol. 23 ›› Issue (2): 152-155.DOI: 10.3969/j.issn.1671-2587.2021.02.004

合肥地区无偿献血人群CD36抗原缺失的调查^*

赵娜娜, 王超, 金惠新, 赵阳, 吕蓉

230031 合肥,安徽省血液中心/合肥市中心血站

收稿日期:2020-09-23 出版日期:2021-04-20 发布日期:2021-04-19
通讯作者: 吕蓉,女,主任技师,主要从事血液免疫和血液安全研究,（E-mail）lvrong612@126.com。
作者简介:赵娜娜（1991-）,女,安徽淮南人,检验师,本科,主要从事输血免疫相关工作,（E-mail）2455975344@qq.com。并列第一作者：王超（1976-）,男,安徽阜阳人,主管检验师,硕士,主要从事分子生物学和输血免疫相关工作,（E-mail）77063566@qq.com。
基金资助:
*本课题受合肥市科技局2019年自主创新“借转补”项目（No.J2019Y05 ）资助

Investigation of CD36 Antigen Deficiency in Unpaid Blood Donors in Hefei

ZHAO Na-na, WANG Chao, JIN Hui-xin, et al

Anhui Blood Center, Hefei 230031

Received:2020-09-23 Online:2021-04-20 Published:2021-04-19

摘要/Abstract

摘要： 目的研究合肥地区无偿献血人群CD36抗原的缺失频率。方法随机留取合肥市中心血站2020年3月~2020年7月血小板无偿捐献者血样973例,流式细胞术检测CD36抗原。EDTA抗凝全血离心制备富血小板血浆,进行血小板CD36抗原检测,下层细胞经红细胞裂解液处理后,进行单核细胞CD36抗原检测。结果 973例血小板无偿捐献者中检出CD36抗原缺失者16例,缺失频率为1.64%;其中Ⅰ型缺失2例,Ⅱ型缺失14例,缺失频率分别为0.20%和1.44%。结论合肥地区无偿献血人群存在一定的CD36抗原缺失。

关键词: 无偿献血者, 血小板, CD36抗原, 流式细胞术

Abstract: Objective To investigate the frequency of CD36 antigen deficiency in blood donors in Hefei. Methods A total of 973 blood samples of healthy platelet donors were randomly collected in Hefei blood center from March 2020 to July 2020. Flow cytometry was used for CD36 antigen detection. Platelet-rich plasma was prepared for detection CD36 antigen on platelets, and the residual cells were treated with erythrocyte lysate for CD36 antigen on monocytes. Results Among 973 platelet donors,16 donors were found platelet CD36 antigen deficiency, of which 2 were type I CD36 deficiency,14 were type II CD36 deficiency with the frequency 0.21% and 1.44% respectively. Conclusion The blood donors population in Hefei has certain CD36 antigen deficiency.

Key words: Blood donors, Platelets, CD36 antigen, Flow cytometry

中图分类号:

R331.1⁺43

赵娜娜, 王超, 金惠新, 赵阳, 吕蓉. 合肥地区无偿献血人群CD36抗原缺失的调查^*[J]. 临床输血与检验, 2021, 23(2): 152-155.

ZHAO Na-na, WANG Chao, JIN Hui-xin, et al. Investigation of CD36 Antigen Deficiency in Unpaid Blood Donors in Hefei[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2021, 23(2): 152-155.

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合肥地区无偿献血人群CD36抗原缺失的调查^*

Investigation of CD36 Antigen Deficiency in Unpaid Blood Donors in Hefei

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