白藜芦醇对小鼠血小板氧化应激和衰老指标及储存时间的影响*

doi:10.3969/j.issn.1671-2587.2021.02.001

临床输血与检验 ›› 2021, Vol. 23 ›› Issue (2): 137-141.DOI: 10.3969/j.issn.1671-2587.2021.02.001

• 基础研究 • 下一篇

白藜芦醇对小鼠血小板氧化应激和衰老指标及储存时间的影响^*

贾彦军, 赵续影, 魏珍珍

061001 沧州市中心血站输血研究室

收稿日期:2019-11-16 出版日期:2021-04-20 发布日期:2021-04-19
作者简介:贾彦军（1982-）,男,主管检验师,硕士,主要从事血型血清学研究,（E-mail）millionaireme@163.com。
基金资助:
*本课题受河北省医学科学研究重点课题计划（No.20181503）资助

Effects of Resveratrol on Platelet Oxidative Stress and Aging Index and Storage Time

JIA Yan-jun, ZHAO Xu-ying, WEI Zhen-zhen

Blood Transfusion Research Office, Cangzhou Central Blood Station, Hebei 061001

Received:2019-11-16 Online:2021-04-20 Published:2021-04-19

摘要/Abstract

摘要： 目的探究白藜芦醇对小鼠血小板氧化应激和衰老指标水平及储存时间的影响。方法选取6周大小的C57 black 6品系小鼠,制备富含血小板的血浆,将每个样本平均分为观察组与对照组,观察组中加入白藜芦醇,并使用二甲基亚砜标定至50 μmol/L;对照组加入等量二甲基亚砜;分别于储存第1、2、3、4、5、6、7 d进行取样测定,检测内容包括：平均血小板体积（MPV）、血小板计数、pH值、葡萄糖水平、活性氧（ROS）水平、SA-β-gal染色阳性细胞比例、血小板表面蛋白CD62P、膜联蛋白Ⅴ及MitoSpy阳性的水平。结果观察期内两组在各时间点上的MPV、血小板计数、pH、葡萄糖水平无明显差异（P>0.05）;储存第6、7天观察组ROS水平显著低于对照组（t=9.977,10.650;P=0.000,0.000）;第2、3、4、5、6、7天观察组SA-β-gal活性强度显著低于对照组（t=3.237,4.004,4.909,3.875,4.392,3.359;P=0.013,0.001,0.000,0.002,0.000,0.010）。两组第1天的血小板分散和聚集水平间不存在统计学差异（P>0.05）,观察组第3天和第5天的血小板分散和聚集水平显著高于对照组（t=3.096,3.396,P=0.015,0.007;t=2.701,3.604,P=0.037,0.004）。观察组在储存第2、3、4、5、6、7天的CD62P活性血小板水平显著低于对照组（t=3.175,3.345,3.851,5.169,4.358,7.601;P=0.017,0.010,0.002,0.000,0.000,0.000）,在储存第3、4、5、6、7天的膜联蛋白V活性血小板水平显著低于对照组（t=13.480,13.260,14.870,3.223,8.716;P=0.000,0.000,0.000,0.015,0.000）,在储存第5、6、7天的MitoSpy阳性血小板水平显著高于对照组（t=4.279,7.702,10.270;P=0.000,0.000,0.000）。结论白藜芦醇可显著改善储存期间小鼠血小板氧化应激、衰老指标、聚集和分散功能及延长储存时间,具有较好的潜在应用价值。

关键词: 白藜芦醇, 血小板, 氧化应激, 衰老

Abstract: Objective To explore the effects of resveratrol on platelet oxidative stress and aging index levels and storage time. Methods Plasma rich in platelets was prepared from 6-week-old C57 black 6 mice. Each sample was equally divided into observation group and control group. The observation group was added with resveratrol, and dimethyl sulfoxide was used to calibrate it to 50 μmmol /L, the control group was added with the same amount of dimethyl sulfoxide. Stored in 1, 2, 3, 4, 5, 6, and 7 days for sampling, testing content including: mean platelet volume（MPV）, platelet count, pH, glucose levels, reactive oxygen species（ROS）level, proportion of Senescence-associatedβ-galactosidase staining positive cells, platelet CD62P, membrane surface proteins associated protein Ⅴ and MitoSpy positive level. Results During the observation period, there was no significant difference in MPV, platelet count, pH, and glucose level between the two groups at each time point（P>0.05）. The ROS level of the observation group at the 5th and 6th day of storage were significantly lower than control group（t=9.977,10.650, P=0.000, 0.000）, the SA-β-gal activity intensity of the observation group at the 2 d, 3 d, 4 d, 5 d, 6 d, 7 d were significantly higher than control group（t=3.237, 4.004, 4.909, 3.875, 4.392, 3.359; P=0.013, 0.001, 0.000, 0.002, 0.000, 0.010）. There was no statistical difference between the platelet spreading and aggregation levels observed at 1d（P>0.05）, the spreading and aggregation levels of the observation group at 3 d and 5 d were significantly above（t=3.096, 3.396, P=0.015, 0.007）. Observation group in the storage of 2 d, 3d, 4 d, 5 d, 6 d, 7 d days of platelet CD62P activity were significantly lower than the control group（t=3.175, 3.345, 3.851, 5.169, 4.358, 7.601, P=0.017, 0.010, 0.002, 0.000, 0.000, 0.000）, the storage of 3 d, 4 d, 5 d, 6 d, 7 d days of membrane protein V activated platelets were significantly lower than the control group（t=13.480,13.260,14.870,3.223,8.716;P=0.000,0.000,0.000,0.015,0.000）, the storage of 5 d,6 d, 7 d MitoSpy positive blood platelet were significantly higher than control group（t=4.279,7.702,10.270;P=0.000,0.000,0.000）. Conclusion sResveratrol can significantly improve platelet oxidative stress, aging index,the spreading and aggregation and the storage time during storage, it has good application potential.

Key words: Resveratrol, Platelets, Oxidative stress, Aging

中图分类号:

R457.1

贾彦军, 赵续影, 魏珍珍. 白藜芦醇对小鼠血小板氧化应激和衰老指标及储存时间的影响^*[J]. 临床输血与检验, 2021, 23(2): 137-141.

JIA Yan-jun, ZHAO Xu-ying, WEI Zhen-zhen. Effects of Resveratrol on Platelet Oxidative Stress and Aging Index and Storage Time[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2021, 23(2): 137-141.

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白藜芦醇对小鼠血小板氧化应激和衰老指标及储存时间的影响^*

Effects of Resveratrol on Platelet Oxidative Stress and Aging Index and Storage Time

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