• 中国科学论文统计源期刊
  • 中国科技核心期刊
  • 美国化学文摘(CA)来源期刊
  • 日本科学技术振兴机构数据库(JST)
临床输血与检验

临床输血与检验 ›› 2020, Vol. 22 ›› Issue (3): 290-294.DOI: 10.3969/j.issn.1671-2587.2020.03.016

• 采供血管理 • 上一篇    下一篇

石家庄地区已知HLA型别单采血小板捐献者基因资料库库容评价*

苏蔓, 李茵, 赵倩, 胡光磊, 王振雷, 何路军   

  1. 050071 石家庄,河北省血液中心
  • 收稿日期:2019-01-10 出版日期:2020-06-20 发布日期:2020-06-13
  • 通讯作者: 王振雷,男,副主任技师,主要从事临床输血研究,（E-mail）lei123456789101112@sina.com。
  • 作者简介:苏蔓（1981-）,女,河北邢台人,副主任技师,博士,主要从事免疫血液学检验工作,（E-mail）manman905@163.com。
  • 基金资助:
    *本课题受2018年度河北医学适用跟踪项目（No.G2018088）资助

Evaluation of A Known HLA Typing Apheresis Platelet Donors Gene Database Pool Size in Shijiazhuang

SU Man, LI Yin, ZHAO Qian, et al   

  1. Organ Transplantation Matching Laboratory, Hebei Blood Center, Shijiazhuang 050071
  • Received:2019-01-10 Online:2020-06-20 Published:2020-06-13

PDF

993

摘要： 目的 分析石家庄地区单采血小板捐献者HLA-I类基因（A、B位点）分布特点,探讨在该地区血小板捐献者基因资料库中找到HLA-I类基因（A、B位点）相合供者的概率并进行血小板捐献者基因资料库最小规模评价。方法 对石家庄地区1 935位无血缘关系的血小板捐献者HLA-I 类基因A、B位点基因分型采用聚合酶链反应-序列特异引物（PCR-SSP）和聚合酶链反应-直接测序分型（PCR-SBT）方法,得到基因型后转化为对应的抗原特异性,计算基因频率和单体型频率,进行血小板捐献者基因资料库最小规模评价,并统计交叉反应组（CREG）表型频率及不配合率。结果 1 935位无血缘关系的血小板捐献者中共检出HLA-A位点抗原特异性19种,其中频率最高的抗原为A2（0.313 4）和A11（0.158 4）;检出HLA-B 位点抗原特异性43种,其中频率最高的为B13（0.118 1）和B61（0.086 3）;观察到HLA-A-B单体型355种,频率大于0.01的共28种,其中频率最高的为A2-B13（0.044 4）和A2-B62（0.029 5）;石家庄地区单采血小板捐献者A-A、A-B抗原CREG表位不配合的概率最高分别为1C-2C和7C-5C,Broad CREG不配合概率最高的为4C-6C。结论 理论上石家庄地区建立一个总库容1 500人的血小板捐献者基因资料库有93.18%的患者有95%的可能性找到至少1例HLA相合的供者,本研究1 935人的基因资料库基本可以满足本地区血小板输注无效患者寻找HLA相合供者的需求。

关键词: 血小板, HLA-I类基因, 基因频率, 单体型, CREG

Abstract: Objective To analyze the distribution characteristics of HLA-I loci (A, B) in apheresis platelet donors in Shijiazhuan area, explore the probability of finding an HLA-matched platelet donor in platelet donor pools in Shijiazhuang area, and evaluate the minimum pool size of platelet donor gene database. Methods HLA-I loci A and B genotypes of 1 935 unrelated platelet donors in Shijiazhuang area were performed by polymerase chain reaction(PCR) using sequence-specific primers (PCR-SSP) and sequence-based typing (PCR-SBT) methods. HLA genotype obtained were converted to antigenic specificity, calculated genetype frequency and haplotype frequency, and evaluated the minimum pool size of platelet donor gene database. The cross-reactive group (CREG) phenotype frequency and its mismatch rate were counted. Results A total of 19 HLA-A locus phenotype specificities were identified in 1 935 unrelated platelet donors, and the highest frequency antigens are A2 (0.313 4) and A11 (0.158 4). A total of 43 B-locus phenotype specificities were identified, and the highest frequency antigens are B13 (0.118 1) and B61 (0.086 3). 355 HLA-A-B haplotypes were observed, of which 28 frequencies were greater than 0.01, the highest frequencies haplotypes were A2-B13 (0.044 4) and A2-B62 (0.029 5). 1C-2C and 7C-5C have the highest mismatch rate of CREG epitopes of A-A and A-B antigens, and 4C-6C has the highest mismatch rate of Broad CREG in apheresis platelet donors in Shijiazhuang area. Conclusion In theory, 93.18% of patients have a 95% ;probability to find at least one HLA-matched donor in a platelet donor pool with a total storage capacity of 1,500 patients. The platelet donor pool size of 1935 would basically meet the needs of patients with platelet transfusion refractoriness in Shijiazhuang area.

Key words: Platelets, HLA-I gene, Gene frequency, Haplotype, CREG

中图分类号: