丙型肝炎患者外周血HCV RNA载量与DARC rs12075多态性的相关性

doi:10.3969/j.issn.1671-2587.2019.01.025

临床输血与检验 ›› 2019, Vol. 21 ›› Issue (1): 73-76.DOI: 10.3969/j.issn.1671-2587.2019.01.025

丙型肝炎患者外周血HCV RNA载量与DARC rs12075多态性的相关性

邵林楠, 梁晓华, 张树婷, 于卫建, 刘铭, 周世航

116001 辽宁省大连市血液中心（邵林楠,梁晓华,张树婷,于卫建,周世航）;
大连医科大学细胞生物学教研室（刘铭）

收稿日期:2018-08-16 出版日期:2019-02-20 发布日期:2019-01-28
通讯作者: 刘铭,女,主要从事遗传相关研究,（E-mail）liuminglinxi@163.com。共同通信作者：周世航,男,主要从事输血相关研究,（E-mail）zshsail@163.com。
作者简介:邵林楠（1984-）,男,河北人,技师,硕士,主要从事输血相关研究,（Tel）0411-82653536（E-mail）sln1984sln@163.com。

Study on the Correlation between HCV RNA Load and DARC rs12075 Polymorphism in HCV Infected Patients

SHAO Lin-nan, LIANG Xiao-hua, ZHANG Shu-ting, et al

Dalian Blood Center,Dalian 116001

Received:2018-08-16 Online:2019-02-20 Published:2019-01-28

摘要/Abstract

摘要： 目的探讨丙型肝炎患者外周血HCV RNA病毒载量与DARC rs12075的相关性。方法以196例大连地区汉族HCV感染者为研究对象,使用TaqMan探针Real-time PCR技术对DARC rs12075进行分型。HCV RNA病毒载量测定采用Roche公司全自动核酸分离纯化检测系统COBAS AmpliPrep/COBAS TaqMan及其配套试剂。用豆形图展示HCV RNA病毒载量。结果 DARC rs12075分型结果显示,FY^*A/FY^*A基因型患者171例,其余为FY^*A/FY^*B基因型,未发现FY*B/FY*B基因型。对FY^*A/FY^*A基因型与FY^*A/FY^*B基因型患者间HCV RNA病毒载量进行比较,差异无统计学意义。进一步根据患者HCV RNA病毒载量不同将其分为4组,分别对各组不同基因型患者间HCV RNA病毒载量进行比较,差异均无统计学意义。结论丙肝患者外周血HCV RNA载量与DARC rs12075多态性无关。

关键词: 丙型肝炎, HCV RNA, 病毒载量, DARC, 多态性

Abstract: Objective To investigate the correlation between HCV RNA load and DARC rs12075 in HCV infected patients. Methods In 196 Dalian Han HCV infected patients,DARC rs12075 polymorphisms were typed by using TaqMan probe Real-time PCR and HCV RNA viral load was measured by Roche COBAS AmpliPrep/COBAS TaqMan with its supporting reagents. The HCV RNA viral load data were presented with beanplots,and the differences were compared through the Mann-Whitney U test. Results DARC rs12075 was genotyped and,171 FY^*A/FY^*A and 25 FY^*A/FY^*B patients were identified. The difference of HCV RNA viral load between FY^*A/FY^*A and FY^*A/FY^*B patients was not significant. In addition,we divided the patients into four groups according to HCV RNA viral load,and compared the HCV RNA viral load between FY^*A/FY^*A and FY^*A/FY^*B patients in each group. But the differences were not statistically significant. Conclusion Our results showed that HCV RNA load in peripheral blood of HCV patients was not related to DARC rs12075 polymorphism.

Key words: Hepatitis C, HCV RNA, Viral load, DARC, Polymorphism

中图分类号:

R512.6⁺3 R392.11

邵林楠, 梁晓华, 张树婷, 于卫建, 刘铭, 周世航. 丙型肝炎患者外周血HCV RNA载量与DARC rs12075多态性的相关性[J]. 临床输血与检验, 2019, 21(1): 73-76.

SHAO Lin-nan, LIANG Xiao-hua, ZHANG Shu-ting, et al. Study on the Correlation between HCV RNA Load and DARC rs12075 Polymorphism in HCV Infected Patients[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2019, 21(1): 73-76.

参考文献

1 Houghton M. The long and winding road leading to the identification of the hepatitis C virus[J].J Hepatol,2009,51(5):939-948.
2 Rehermann B. Hepatitis C virus versus innate and adaptive immune responses:a tale of coevolution and coexistence[J]. J Clin Invest,2009,119(7):1745-1754.
3 Zeisel M B,Fafi-Kremer S,Robinet E,et al. Adaptive immunity to hepatitis C virus[J]. Viruses,2009,1(2):276-297.
4 Grodecka M,Bertrand O,Karolak E,et al. One-step immunopurification and lectinochemical characterization of the Duffy atypical chemokine receptor from human erythrocytes[J]. Glycoconj J,2012,29(2-3):93-105.
5 Berres M L, Trautwein C, Schmeding M,et al. Serum chemokine CXC ligand 10 (CXCL10) predicts fibrosis progression after liver transplantation for hepatitis C infection[J]. Hepatology,2011,53(2):596-603.
6 邵林楠,张树婷,于卫建,等. 大连地区汉族人群Duffy血型系统基因型分布[J]. 临床血液学杂志,2017,30(4):261-263.
7 Kampstra P. Beanplot:A Boxplot Alternative for Visual Comparison of Distributions[J]. J Stat Softw,2008,28(1):1-9.
8 苏咪咪. 大数据的“豆形”可视化及其在资本市场中的应用[J]. 科学与管理,2014,34(6):3-7.
9 刘妲妲,王全楚.输血后丙型肝炎患者的临床特点及自然病程[J]. 实用肝脏病杂志,2008,11(6):410-411.
10 Rot A. Contribution of Duffy antigen to chemokine function[J]. Cytokine Growth Factor Rev,2005,16(6):687-694.
11 He W,Neil S,Kulkarni H,et al. Duffy antigen receptor for chemokines mediates trans-infection of HIV-1 from red blood cells to target cells and affects HIV-AIDS susceptibility[J]. Cell Host Microbe,2008,4(1):52-62.
12 Shen H,Schuster R,Stringer K F,et al. The Duffy antigen/receptor for chemokines (DARC) regulates prostate tumor growth[J]. FASEB J,2006,20(1):59-64.
13 Sellami M H,Chaabane M,Kaabi H,et al. Evidence that erythrocyte DARC-positive phenotype can affect the GVHD occurrence after HLA-identical sibling HSCT[J]. Transpl Immunol,2011,25(2):148-152.
14 Miller L H,Mason S J,Clyde D F,et al. The resistance factor to Plasmodium vivax in blacks:the Duffy-blood-group genotype,FyFy[J]. N Engl J Med,1976,295(6):302-304.
15 王敏,乐晓华,韩红星,等. HCV-RNA病毒载量和肝脏病理的关系[J]. 实用医学杂志,2006,22(13):1513-1514.
16 丁柳,周易,宋兴勃,等. HCV RNA 载量与肝脏组织损伤的关系[J]. 中国实验诊断学,2011,15(3):481-483.
17 Schnabel R B,Baumert J,Barbalic M,et al. Duffy antigen receptor for chemokines (Darc) polymorphism regulates circulating concentrations of monocyte chemoattractant protein-1 and other inflammatory mediators[J]. Blood,2009,115(26):5289-5299.

[1]	桂嵘, 王勇军, 黄雪原, 马金旗, 赵国胜, 王露楠, 王学锋, 杨瑞锋. 关于目前术前/输血前患者感染性疾病筛查的思考与探索^*[J]. 临床输血与检验, 2020, 22(6): 565-567.
[2]	程金凤, 焦巍, 周红霞, 周俊, 孟芹, 凌锋, 蔡丹, 黄远帅, 邓智华, 王顺, 陈亚军, 唐曙明, 谢小兵, 李志波, 黄丽芳, 王占科, 吕小英, 江小工, 谢华斌, 周明, 骆群, 王勇军, 桂嵘, 陈军浩, 王海燕. 术前/输血前患者乙型肝炎病毒、丙型肝炎病毒、人类免疫缺陷病毒核酸检测多中心研究^*[J]. 临床输血与检验, 2020, 22(6): 568-573.
[3]	李红燕, 唐曙明, 李志波, 谢华斌, 王勇军, 桂嵘, 陈军浩, 王海燕, 凌锋, 王顺, 黄丽芳, 邓智华, 蔡丹, 程金凤, 焦巍, 周红霞, 骆群, 黄远帅, 周明, 吕小英, 孟芹, 江小工, 谢小兵, 王占科, 陈亚军. 术前及输血前HCV血清学检测与核酸检测结果不一致分析^*[J]. 临床输血与检验, 2020, 22(6): 574-578.
[4]	董航, 黄雪原, 贺理, 黄丽芳, 邓中平, 王海燕, 王勇军, 王顺, 凌锋. 多中心HBV、HCV、HIV血清学检测弱反应性标本的核酸检测结果分析^*[J]. 临床输血与检验, 2020, 22(6): 579-582.
[5]	成枫, 许宏, 肖淑欣, 赵洪国. HPA-1-6/17基因多态性与青岛市血小板减少症遗传易感性的关系分析[J]. 临床输血与检验, 2020, 22(5): 521-526.
[6]	刘英超, 张秋月, 刘琳, 任更朴. 丙肝病毒核心抗原检测的临床价值及其与HCV-RNA、ALT、AST的关系研究[J]. 临床输血与检验, 2020, 22(3): 316-318.
[7]	魏晓珠, 苏维, 陈望, 刘爱玲. 沿海地区人群高尿酸血症患病率及其与SLC2A9基因rs2241480位点多态性的关系[J]. 临床输血与检验, 2020, 22(2): 160-164.
[8]	叶芳, 黄婷婷, 徐徽, 蒋浏馨, 伊兴旭, 王宏志, 凡任芝. 叶酸代谢相关酶基因多态性与不良孕产的关系研究[J]. 临床输血与检验, 2019, 21(5): 514-516.
[9]	梁志洪, 谢敬文, 阮丽芬, 蓝文莉, 陈结贞, 古文鑫, 陈永雪. 临床稀有血型孕妇RHD基因分型研究^*[J]. 临床输血与检验, 2019, 21(4): 348-351.
[10]	苏蔓, 王振雷, 胡光磊, 李茵, 张泓楠, 何路军. 1 914名河北地区汉族人群HLA-DQB1基因多态性分析^*[J]. 临床输血与检验, 2019, 21(4): 380-382.
[11]	苏蔓, 胡光磊, 李茵, 郭霞, 张泓楠, 王振雷, 何路军. HLA-DRB1基因和河北汉族终末期肾衰竭的相关性研究[J]. 临床输血与检验, 2019, 21(3): 268-270.
[12]	何智. 乌鲁木齐地区哈萨克族献血人群HPA-15基因多态性分析[J]. 临床输血与检验, 2019, 21(2): 152-154.
[13]	张如, 朱建华, 李强. HCV RNA检测在丙型肝炎诊断中的应用价值[J]. 临床输血与检验, 2019, 21(2): 200-202.
[14]	朱蓉, 王峰, 梁静, 帕提古丽, 苏来曼, 赖薇兰炯采, 邱芬. 新疆柯尔克孜族人群红细胞ABO、RH系统抗原分布及遗传多态性调查^*[J]. 临床输血与检验, 2019, 21(1): 36-38.
[15]	戴菊华,乔蕊,张捷. 抗栓治疗的药物基因组检测[J]. 临床输血与检验, 2018, 20(3): 333-336.

丙型肝炎患者外周血HCV RNA载量与DARC rs12075多态性的相关性

Study on the Correlation between HCV RNA Load and DARC rs12075 Polymorphism in HCV Infected Patients

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

关于本刊

作者中心

在线期刊

访问统计

联系我们