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临床输血与检验

临床输血与检验 ›› 2018, Vol. 20 ›› Issue (5): 482-486.DOI: 10.3969/j.issn.1671-2587.2018.05.009

• 临床输血 • 上一篇    下一篇

石家庄地区无偿献血者HTLV-Ⅰ/Ⅱ检测情况分析

李莉华, 戚海, 陈莉, 马印图   

  1. 050071 河北省血液中心（李莉华,戚海,陈莉）;
    解放军白求恩国际和平医院输血科（马印图）
  • 收稿日期:2018-02-23 出版日期:2018-10-20 发布日期:2018-09-27
  • 通讯作者: 马印图,男,副主任技师,主要从事临床输血风险控制研究,（E-mail）13722793663@163.com。
  • 作者简介:李莉华（1971-）,女,河北石家庄人,副主任检验师,学士,主要从事血液安全研究,（Tel）0311-87044310（E-mail）15931096399@163.com。

Analysis of Screening for HTLV-Ⅰ/Ⅱ in Voluntary Blood Donors in Shijiazhuang

LI Li-hua, QI Hai, CHEN Li, etal   

  1. Hebei Blood Center 050071
  • Received:2018-02-23 Online:2018-10-20 Published:2018-09-27

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摘要： 目的 分析石家庄地区无偿献血者中人类嗜T淋巴细胞白血病病毒（HTLV）检测结果,为本地区临床用血安全提供科学依据。方法 采用ELISA法（双抗原夹心）对河北省血液中心2016年9~12月采集的献血者标本进行HTLV-Ⅰ/Ⅱ抗体筛查,初筛反应性标本再进行双孔复试。复试阳性标本送卫生部临床检验中心做相关附加试验。结果 石家庄地区41 000人份献血者标本中初筛阳性标本25例,经RT-PCR和蛋白印迹法（western blot,WB）确证阳性1例。该试剂假阳性率为0.06%。对献血者进行追溯,确认该献血者为无症状固定献血者,已有4次合格献血经历。结论 石家庄地区无偿献血者属于HTLV-Ⅰ/Ⅱ感染的非流行区或低发区。采用ELISA法进行HTLV-Ⅰ/Ⅱ抗体筛查,其结果假阳性率低,适用于大规模血液筛查和血清流行病学调查。

关键词: 人类嗜T淋巴细胞白血病病毒Ⅰ/Ⅱ, 无偿献血者, 血液筛查

Abstract: Objective To evaluate and analyse the testing result of HTLV-Ⅰ/Ⅱ infection in Shijiazhuang district in order to provide a scientific basis for clinical blood transfusion safety in this area. Methods Antibody of HTLV-Ⅰ/Ⅱ was measured with double antigen sandwich enzyme-linked immunosorbent assay （ELISA）kit from Sep to Dec in 2016 in Hebei blood center. The positive blood samples in the first test were re-tested by double-hole retrial method．Then the sero-positive samples were confirmed by RT-PCR or Western blot in National Center for Clinical Laboratories. Results Among 41 000 donors in Shijiazhuang district,25 cases were detected positive in the first test but only one case were confirmed positive with RT-PCR or WB. Retrospective investigation revealed that this HTLV sero-positive donor had had the eligible records as high as four blood donations in Hebei blood center. Conclusions These results indicate that Shijiazhuang is non-endemic for HTLV-I as well as HTLV-II. ELISA method can be used for HTLV-Ⅰ/Ⅱscreening to epidemiological investigation,due to the low rate of false positives.

Key words: HTLV-Ⅰ/Ⅱ, Voluntary blood donors, Blood screening

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