• 中国科学论文统计源期刊
  • 中国科技核心期刊
  • 美国化学文摘(CA)来源期刊
  • 日本科学技术振兴机构数据库(JST)

临床输血与检验 ›› 2018, Vol. 20 ›› Issue (6): 643-646.DOI: 10.3969/j.issn.1671-2587.2018.06.028

• 血型与临床 • 上一篇    下一篇

毛细管超速离心法在输血后患者Rh血型鉴定中的应用分析

刘丽娜, 尹文, 胡兴斌   

  1. 710032 西安,第四军医大学西京医院输血科
  • 收稿日期:2018-02-19 出版日期:2018-12-20 发布日期:2018-11-12
  • 作者简介:刘丽娜(1984-),女,山西运城人,输血技师,学士,主要从事临床输血工作,(Tel)13649246379。

Application of Capillary Ultracentrifugation in Identification of Rh Blood Group in the Patients after Transfusion

LIU Li-na, YIN Wen, HU Xing-bin   

  1. Department of Transfusion,Xijing Hospital,No.4 Military Medical University,Xi'an,710032
  • Received:2018-02-19 Online:2018-12-20 Published:2018-11-12

摘要: 目的 探讨毛细管超速离心技术在输血后患者Rh血型鉴定中的应用。方法 对送检的10例抗体筛选阳性、Rh抗原鉴定为混合视野的患者红细胞,洗涤制备压积红细胞,应用毛细管超速离心技术进行分离,分别取近心端和远心端红细胞进行Rh血型鉴定。结果 10例患者标本经毛细管离心分离后,均能准确鉴定Rh血型,而且可以辅助鉴定抗体类型。结论 毛细管超速离心技术可较好地分离输血后患者红细胞,进而准确鉴定患者血型,值得在临床输血中推广使用。

关键词: 毛细管超速离心法, 输血, Rh血型鉴定

Abstract: Objective To investigate the feasibility of capillary ultracentrifugation for identification of Rh blood group in patients after transfusion. Methods Erythrocytes of 10 patients with positive antibody screening and Rh antigen confirmed in the mixed field of view were washed to prepare pressed red blood cells. The blood cells were isolated by capillary ultracentrifugation. The Rh blood group was identified using both proximal and distal red blood cells. Results Ten specimens from the patients after capillary centrifugal separationwere accurately identified as Rh blood group,with its capability of identifying antibody types. Conclusion Capillary ultracentrifugation technique can isolate the red blood cells of patients following transfusion and correctly indicate the blood type of patients,being applicable to clinical blood transfusion.

Key words: Capillary ultracentrifugation, Transfusion, Rh blood group identification

中图分类号: