关键词: 人类免疫缺陷病毒, 化学发光免疫试验, 免疫印迹试验

Abstract: ObjectiveTo evaluate the specificity of chemiluminescence assay (CLIA) in anti-HIV test, and to analyze HIV infection in the patients before blood transfusion. MethodsCLIA was used to detect the anti-HIV in 73727 cases from pre-transfusion patients. The sera with initially positive anti-HIV was then confirmed by Western blotting (WB). ResultsOne hundred and forty f serum samples were anti-HIV positive by CLIA. Among them, 110 were confirmed to be positive by WB assay. Anti-HIV was more prevalent in those aged 20-60 years, and the HIV infection rate of male is much higher than that of female (χ2=15.01,P<0.005). In the samples with S／CO value between 1 and 10, the seroprevalence of anti-HIV was 0%, while in the sample with S／CO value more than 50, the seroprevalence of anti-HIV was 100% tested with WB assay. The anti-HIV positive rate of WB assay showed significant difference among 1<S／CO≤10, 10<S／CO≤50 and S／CO>50 three groups (P<0.005). ConclusionsPart of blood recipients before transfusion have HIV infection. Low S／CO value indicates relatively high false positive HIV infection although CLIA shows high sensitivity.

Key words: Human, immunodeficiency, virus, Chemiluminescence, assay, Western, blot