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临床输血与检验

临床输血与检验 ›› 2020, Vol. 22 ›› Issue (5): 510-514.DOI: 10.3969/j.issn.1671-2587.2020.05.016

• 采供血管理 • 上一篇    下一篇

淄博地区血小板捐献者HLA-A,B和HPA1-17分型资料库的建立*

周博, 田茂生, 马晨, 任晓朦, 朱传福   

  1. 255033 淄博市中心血站（周博,田茂生,马晨,任晓朦）;
    山东省血液中心（朱传福）
  • 收稿日期:2019-04-03 出版日期:2020-10-20 发布日期:2020-10-16
  • 通讯作者: 田茂生,男,主任医师,主要从事临床输血管理,（E-mail）18853386188 @163.com。
  • 作者简介:周博（1972-）,男,山东蓬莱人,副主任技师,学士,主要从事免疫血液学研究,（E-mail）18853386188@163.com。
  • 基金资助:
    *本课题受山东省医药卫生科技发展基金项目（No.2013W-S0169）、淄博市科学技术发展计划项目（No.2017kj010013）资助

Establishment of HLA-A, B and HPA1-17 Genetic Database of Platelet Donors in Zibo Area

ZHOU Bo, TIAN Mao-sheng, MA Chen, et al   

  1. Zibo Blood Center,Zibo 255033
  • Received:2019-04-03 Online:2020-10-20 Published:2020-10-16

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摘要： 目的 了解淄博地区血小板捐献者人类白细胞抗原（human leukocyte antigen,HLA）A,B位点和人类血小板抗原（human platelet antigen,HPA）1-17系统基因多态性分布特点,初步建立淄博地区血小板捐献者已知 HLA、HPA 基因型资料库。 方法 分别用聚合酶链反应-碱基序列直接测序（polymerase chain reaction-sequencing based typing,PCR-SBT）和 聚合酶链反应-序列特异性引物（polymerase chain reaction-sequence specific primer, PCR-SSP）方法对200名无血缘关系的单采血小板捐献者进行HLA-A,B和HPA1-17系统基因分型,计算基因频率、基因型频率。 结果 共检出HLA-A位点低分辨等位基因15个,HLA-B 位点低分辨等位基因29个,其中HLA-A位点基因频率居前五位的是A*02、A*24、 A*11、A*30、A*33,基因频率分别为0.260 0、0.172 5、0.137 5、0.135 0、0.070 0; HLA-B位点基因频率居前五位的是B*13、B*15、B*40、B*44、B*51,基因频率分别为0.160 0、0.132 5、0.130 0、0.067 5、0.062 5。HPA1-17中,HPA4a 、7a-14a、16a、17a呈单特异性,未检测出相应的对偶基因抗原HPA-b;在HPA1-17中,杂合度最高的是HPA15,基因型HPA15a /15a、HPA15a /15b、HPA15b/15b频率分别是0.275 0、0.530 0、0.195 0。 结论 分析探知了淄博地区血小板捐献者HLA-A,B和 HPA1-17的分布特点。本研究将为建立山东省区域性血小板捐献者资料库提供帮助。

关键词: 人类白细胞抗原, 人类血小板抗原, 基因频率, 血小板捐献者资料库

Abstract: Objective To understand the genetic polymorphism of HLA-A,B alleles and HPA1-17 in platelet donors in Zibo area, and establish a database of platelet donors with HLA and HPA locus. Methods Polymerase chain reaction-sequencing based typing (PCR-SBT) and Polymerase chain reaction-sequence specific primer (PCR-SSP) methods were applied in genotyping for HLA-A,B and HPA1-17, respectively.Total of 200 blood samples were collected from unrelated platelet donors．The gene frequencies of HLA-A、B and HPA1-17 were calculated using direct counting method. Results The numbers of low-resolution alleles identified were 15 for HLA-A and 29 for HLA-B,respectively. The most frequent alleles at HLA-A locus were A*02、A*24、A*11、A*30、A*33,with a gene frequency in 0.260 0、0.172 5、0.137 5、0.135 0、0.070 0,respectively．For HLA-B locus,the common alleles were B*13、B*15、B*40、B*44、B*51,with a gene frequency of 0.160 0、0.132 5、0.130 0、0.067 5、0.062 5,respectively.Within HPA4a, 7a-14a, 16a and 17a systems, only HPA-aa genotype were found. Findings of HPA-15 showed the greatest heterozygosity with a genotype frequency of 0.275 0、0.530 0 and 0.195 0 for HPA15a/15a,HPA15a/15b,and HPA15b /15b,respectively. Conclusion The distribution of HLA-A,B and HPA1-17 in platelet donors in Zibo area have been explored. This research can contribute to establish a regional bank of platelet donors in Shandong province.

Key words: HLA, HPA, Gene frequency, Bank of platelet donors

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