A Retrospective Analysis on Nucleic Acid Screening of Blood in Shanghai Region after Centralized Detections

doi:10.3969/j.issn.1671-2587.2016.03.024

JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE ›› 2016, Vol. 18 ›› Issue (3): 276-280.DOI: 10.3969/j.issn.1671-2587.2016.03.024

Previous Articles Next Articles

A Retrospective Analysis on Nucleic Acid Screening of Blood in Shanghai Region after Centralized Detections

LU Tao-hong, ZHOU Guo-ping, ZHANG Xi

Shanghai Blood Center, Shanghai, 200051

Received:2016-03-14 Online:2016-06-20 Published:2016-09-21

Abstract

Abstract: Objcective To investigate the positive impact of nuclear acid testing on reducing the risk of regional infectious transfusion through systematic retrospective analysis on nucleic acid screening data of blood in Shanghai after centralized detections. Method Nucleic acid testing（NAT） results of 1,276,206 blood samples with no reactive to the Enzyme-Linked ImmunoSorbent Assay (ELISA) (HBsAg、Anti-HCV、HIV Ag-Ab) from various blood services in Shanghai region after centralized screening are analyzed. Results The ratio of “ negative in ELISA (HBsAg,Anti-HCV, and HIV Ag-Ab) but positive in nucleic acid testing (NAT)” ((ELISA NR/NAT R ) in Shanghai was found to be 0.63‰. The overall ELISA NR/NAT R ratios from the year 2012 to 2015 gradually declined . The highest ELISA NR/NAT R ratio was in 2012 (0.76‰), apparently higher than those of the next 3 years（P<0.01）, and the second highest ELISA NR/NAT R ratio appeared in 2013 and 2014 (0.62‰). The lowest ELISA NR/NAT R ratio (0.50‰) was observed in 2015. There was no significant differenceduring the last 3 years (P>0.05). Among the total 800 cases of reactive “NAT” (0.63‰), 464 (0.36‰) cases were positive for HBV DNA, with the highest ratio of HBV DNA positive cases in 2012 (0.49‰), which was notably higher than those of the next 3 years (P<0.01). The overall ratios of HBV DNA positive cases from 2013 to 2015 were ranged from 0.31‰ to 0.34‰, with no significant difference (P>0.05). For other cases of reactive “NAT”, 7 (0.005‰) were positive for HCV RNA, 14 (0.01‰) were positive for HIV RNA, and 315 (0.25‰) were negative for the three viruses. Conclosion Despite the exist of certain false reactivity in NAT, the full implementation of nuclear acid testing for blood in Shanghai region after centralized detections has effectively reduced the risk in blood transfusion and ensure the blood safety citywide.

Key words: Centralized, screening, Nucleic, acid, testing, Blood, safety

CLC Number:

R392.11R457.1+2

LU Tao-hong, ZHOU Guo-ping, ZHANG Xi. A Retrospective Analysis on Nucleic Acid Screening of Blood in Shanghai Region after Centralized Detections[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2016, 18(3): 276-280.

References

[1] Mine H,Emura H,Miyamoto M,et al.High throughPut screening of 16 million serologically negative blood donors for hePatitis B virus,hePatitis C virus and human immunodeficiency virus tyPe-1 by nucleic acid testing with sPecific and sensitive multiPlex reagent in JaPan[J].J Virol Methods,2003,112（1-2）:145-151.
[2] Hourfar M K., Jork C, Schottsted V,et al. ExPerience of German Red Cross blood donor services with nucleic acid testing: results of screening more than 30 million blooddonations for human immunodeficiency virus-1, hePatitis C virus, and hePatitis B virus[J]. Transfusion, 2008,48(8):1558-1566.
[3] Nübling C. Micha, Heiden Margarethe, Chudy Michael, et al. ExPerience of mandatory nucleic acid test (NAT) screening across all blood organizations in Germany: NAT yield versus breakthrough transmissions[J]. Transfusion, 2009,49(9):1850-1858.
[4] Stramer SL, Wend U, Candotti D, et al. Nucleic Acid Testing to Detect HBV Infection in Blood Donors[J]. N Engl J Med, 2011,364（3）:236-247.
[5] Taira R,Satake Masahiro,Momose S,et al. Residual risk of transfusion-transmitted hePatitis B virus(HBV) infection caused blood comPonents derived from donors with occult HBV infection in JaPan[J].T ransfusion ,2013, 53(7):1393-1404.
[6] 周国平王迅任亚娜,等. 血液筛查策略的选择要因地制宜[J]. 中国输血杂志, 2015, 28(1):108-110.
[7] 邹峥嵘,周国平,朱永明. 血站血液集中化检测的实践与思考[J]. 中国输血杂志, 2014,27(11):1085-1087.
[8] 白玉,常卫红.美国应用核酸检测筛查血液的历程和现状[J].国际生物制品学杂志,2009,32（2）：101-105.
[9] 任芙蓉.核酸检测技术在国内外血液筛查中的应用[J].北京医学,2008,30（8）：561-564.
[10] 陈兴智,许建荣,吴敬林,等. 血液标本集中检测提高输血安全性研究[J]. 检验医学与临床, 2014,11(3): 299-301.
[11] 吴丹霄,吴亚玲,吕杭军.核酸检测在献血筛查中的应用[J].浙江预防医学,2015,27(5):501-503.
[12] 吕蓉,盛琪琪,赵阳,等. 核酸检测技术在安徽省血液中心筛查种的应用分析[J].安徽医学,2014,35（5）:572-573.
[13] 柯苑,赵静,马成平,等.核酸检测技术在献血人员血液筛查中的应用[J].检验医学与临床志,2014,11（20）：2858-2859.
[14] 段勇 ,郭逸 ,叶世辉. 病毒核酸检测技术在西安地区血液筛查中的应用分析[J].中国输血杂志, 2014,27(8):842-844）.
[15] 张妍,朱海峰,孙波,等. 核酸检测技术在血液筛查中的应用及分析[J] . 中国输血杂志, 2012,25(12):1298-1300.
[16] 16 黎蕾. 献血者中的隐匿性HBV感染[J].中国输血杂志,2010,23（10）：905-906.
[17] 周国平,谢云峥,王迅,等. 做好假反应性献血者归队是血站的责任[J]. 中国输血杂志, 2014,27(10):1079-1082.

A Retrospective Analysis on Nucleic Acid Screening of Blood in Shanghai Region after Centralized Detections

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

About

Author

Journal

Access Statistics

Contact

[1]	ZHAO Na-na, WANG Chao, JIN Hui-xin, et al. Investigation of CD36 Antigen Deficiency in Unpaid Blood Donors in Hefei [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2021, 23(2): 152-155.
[2]	CHI Hong-xu, JIANG Ying, YAO Huan, et al. Analysis of the Changes and Influencing Factors of Blood Cells before and after the Collection of Mononuclear Cells in Patients with Non-Hodgkin's Lymphoma [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2021, 23(2): 156-162.
[3]	WANG Hui-ru, LIU Hui-lan. Factors Influencing Blood Transfusion in Patients after Unrelated Umbilical Cord Blood Transplantation [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2021, 23(2): 162-166.
[4]	TIAN Jing, CHEN Feng, LIU Xiao-min, et al. Risk Factors for Adverse Reactions and Strategies for Nursing in Perioperative Autologous Blood Donation [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2021, 23(2): 173-177.
[5]	LIU Qian, WANG Xiao-huan, GUO Kai, et al. The Change of Laboratory Indexes after Exchange Transfusions for Neonates [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2021, 23(2): 177-180.
[6]	WANG Lei, WU Xue-zhong. Retrospective Analysis of Rh (D,C, c, E, e) Antigens Compatibility between Blood Recipients and Donors [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2021, 23(2): 181-185.
[7]	YANG Chun-chen, RONG Rui-ming, LI Yang, et al. An Evaluation Model of Clinical Blood Transfusion Records Based on Cov-AHP-fuzzy Comprehensive Evaluation Method [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2021, 23(2): 192-196.
[8]	GUO Wei-jie, LIU Ze-ya, ZHANG Fan, et al. Meta-analysis：Unexpected Antibody Positive Rate of Blood Donors in China [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2021, 23(2): 202-212.
[9]	LIN Shou, JIANG Wei-mei, ZHAN You-zhi, et al. Epidemiological Characteristics of Recent HIV-1 Infection in Blood Donors in Fuzhou Area [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2021, 23(2): 212-215.
[10]	WANG Wen-ting, DANG Pan-yu, ZHANG Bao-ping, et al. Transfusion Strategy and Prognosis of Hemolytic Disease of the Fetus and Newborn Caused by Anti-D Antibody [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2021, 23(1): 38-40.
[11]	ZENG Yi-mei, LEI Hang, WANG Yu-qin, et al. ABx Subtype Formation by 1,3-D-Galactosyltransferase p.Arg187Cys Mutation [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2021, 23(1): 41-45.
[12]	FU Hao-xuan, FU Hua-lai, WANG Jie, et al. Effect of Temperature Difference in Gas Phase Liquid Nitrogen Tank on the Preservation Quality of Umbilicalcord Blood Hematopoietic Stem Cells [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2021, 23(1): 50-54.
[13]	ZHONG Ming-lu, HUANG Hui-ying, ZHANG Yang, et al. The Efficacy and Safety of Preoperative Autologous Blood Donation in Spinal Surgery [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2021, 23(1): 68-71.
[14]	LI Li-wei, ZHONG Ning, LI Xing-long, et al. Research on Establishment and Application of Blood Transfusion Sample Distribution Management Model Based on Information System [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2021, 23(1): 79-82.
[15]	ZHU Lin-lin, ZHAO Qing-wei, ZHANG Chen-guang. Practice of CBL Based on Research Study in The Teaching of Clinical Blood Transfusion Laboratory Medicine [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2021, 23(1): 104-106.