Abstract: Objective To study the distribution and drug resistance ofKlebsiellapneumoniae in hospital and analyze the genotypes of K.pneumoniaewith biofilm. Methods One hundred and sixty strains of clinical specimens were collectedfrom August 2013 to August 2015.The bacterial distribution,drug resistance and K.pneumoniae genotype were analyzed. Results K.pneumoniaedominated in the specimensfrom respiratory tract and urethra,accounting for 59.38% and 22.88%,respectively.The highest rate of ICSI was 40.63%,followed by 16.25% and 14.38%.K.pneumoniaeresistance to ampicillin accounted for 80.00%,followed by cefazolin（67.50%）,and ceftriaxone,（65.00%）.More than 50% of the strains tested showed relatively resistant to ampicillin/sulbactam,sulfamethoxazole and cefotaxime.Antibiotics to which K.pneumoniaeproduced less resistance included erpicane,followed by imipenem and cefotetan.The biofilm positive rate in all 160 strains was 37.50%.The ESBLs detected 16 strains of K.pneumoniae biofilm,41.75% of the bacteria presented positive TEM gene and 31.25%,SHV gene while bacteria carrying both SHV and TEM genes accounted for 12.50%.No CTX2Mwas detected. Conclusion The incidence of K.pneumoniae infection in intensive care unit is relatively high,and the drug resistance of the bacteria seems to be low.The predominant genotypes of K.pneumonia with biofilm include SHVand TEM.

Key words: Klebsiellapneumonia, Biofilm, Genotype, Drug, resistance