Abstract: Objective To detect and analyze the human parvovirus B19 antigens and antibodies in methylene blue inactivated virus-free plasma to evaluate the safety of blood transfusion. Method 1 056 bags of plasma inactivated with methylene blue were randomly selected and prepared from February to April,2016,for detection of the human parvovirus B19 antigens and antibodies using ELISA. Samples of the positive HPV B19 antibodies were grouped for statistical analyses according to gender,age,and blood groups. Results Parvovirus B19 virus antigens were negative in all 1056 bags of methylene blue inactivated virus-free plasma,whereas IgG and IgM antibodies were positive with the overall prevalence of 20.5%（216/1056）,with 19.5%（206/1056）IgG positive rate being,and 4.9%（52/1056）IgM positive rate. The HPV B19 antibody positive rate were found to be different among different blood donors：B19 positive rate increased with age,antibodies to B19 in blood group A and AB was higher than in groups B and O.No statistically significant difference was noted in gender. Conclusion Although the risk of Yichang residents at human parvovirus B19 infection from blood transfusion is not high,detection of HPV B19 before blood transfusion is significant for some recipients such as immunocompromised patients.

Key words: Methylene blue inactivated virus-free plasma, Human parvovirus B19, Blood safety