Abstract: Objective To analyze the sources of volunteer donators and testing results of their blood in recent five years in order to provide a reference for developing the effective recruitment strategy of blood donation. Methods A total of 158 027 donators were collected，including local residents，soldiers，and the relatives of patients. Preliminary examinations and retests of whole blood samples were performed for serum alanine aminotransferase （ALT），hepatitis B virus （HBV），hepatitis C virus （HCV），human immunodeficiency virus （HIV）and Treponema pallidum （TP）. The sources of the donors and testing results were statistically analyzed. Results Among 158027 voluntary blood donators，the proportion of the local residents （in the street），soldiers，and the relatives of patients was 1∶2.7∶4.4，the total failure rate was 4.1%. The failure rate of donations in the residents and soldiers was lower than those in the relatives and friends of patients（χ2=22.329，P＜0.05）. The difference of total unqualified rate in different years was noted（χ2= 519.222，P＜0.05）. The unqualified rate was found to be HBsAg>ALT>TP>HCV>HIV. The difference of unqualified rate in all tested markers （except for HCV）in different years was statistically significant（χ2=2 298.798、41.849、23.547、34.582，P＜0.05）. Nine cases showed positive HIV and HBV，29 cases had positive HIV and TP，and 1 case exhibited positive HIV，TP and HCV，which is statistically different in the three populations of blood donation（χ2=52.707，P＜0.05）. Conclusion Continuous attention should be paid to the blood detections and blood-borne infectious diseases for ensuring the safety of blood sources.

Key words: Blood donation, Concurrent infection, Blood detection