Abstract: Objective Anti-G antibodies in prenatal serological testing were identified to distinguish anti-G from anti-D and both anti-D and anti-C antibodies. O-type ccDEE and Ccdee cells were used as adsorbed cells, and“double adsorptions”was used. Methods The ccDEE and Ccdee cells less reported were selected as adsorbed cells, double adsorptions were performed. ABO blood typing, Rh typing, direct Combs test, irregular antibody screening and antibody titer detection and other tests were used to identify Anti-G. Results The experimental design and method can effectively identify anti-G and distinguish anti-D and anti-C from anti-G. Conclusion O-type ccDEE and Ccdee cells were used as adsorbed cells. The double adsorptions were used to identify Anti-G. The cell source was wide, the absorption time was saved, and the identification result was accurate.

Key words: Prenatal serological examination of pregnant women, Identification of irregular antibodies, Anti-G antibodies, Absorption-elution test