Research of Ael Subgroup Gene Polymorphism

doi:10.3969/j.issn.1671-2587.2022.04.003

JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE ›› 2022, Vol. 24 ›› Issue (4): 422-426.DOI: 10.3969/j.issn.1671-2587.2022.04.003

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Research of Ael Subgroup Gene Polymorphism

LI Xiao-fei, ZHANG Ye, LIU Ting-ting, et al

Beijing Red Cross Blood Center, Beijing 100088

Received:2022-03-11 Online:2022-08-20 Published:2022-08-19

Abstract

Abstract: Objective To study Ael subgroup gene polymorphism and correspondingly mutation mechanism. Methods 9 cases of suspected Ael/AelB subgroups detected by tube method were further identified by absorption-elution test, Real time PCR and gene sequencing for accurate ABO blood type results.Results There were 4 kinds of alleles (Ael02, Ael04, Ael05 and Ael10) detected through the nucleotide sequences analysis and alignment, which were generated by corresponding mutation mechanisms (gene recombination, splicing error, point-mutation and frameshift mutation).Conclusion The conformation of Ael subgroup show high genetic diversity and different mutation mechanisms leading to the affected activity of A glycosyltransferase.

Key words: Ael subgroup, Mutation mechanism, Allele, Polymorphism

CLC Number:

R457.1⁺1

LI Xiao-fei, ZHANG Ye, LIU Ting-ting, et al. Research of Ael Subgroup Gene Polymorphism[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2022, 24(4): 422-426.

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