Abstract: Objective To investigate the distribution of Rh phenotype among RhD positive blood donors in Liuzhou City, and to create a database for these phenotypes to aid in the blood matching with rare blood types. Methods We examined 10 870 blood samples from RhD positive unpaid blood donors in Liuzhou between September 2020 and July 2024. The presence of RhD, C, c, E and e antigens was determined using serological methods. The data obtained were used to construct a Rh phenotype database for blood donors by means of a laboratory information management system. Results Eight different Rh phenotypes were detected among the 10 870 RhD positive blood donors studied. The frequency of these phenotypes, listed from most to least common, was CCee (60.55%), CcEe (25.58%), Ccee (8.20%), ccEE (2.43%), ccEe (1.90%), CCEe (0.96%), ccee (0.22%), and CcEE (0.16%). Notably, no CCEE phenotype was observed. The positive rates of RhC, c, E and e antigens from high to low were e (97.41%), C(95.46%), c (38.49%), and E (31.03%). Conclusion The primary Rh phenotypes of RhD positive blood donors in Liuzhou are CCee and CcEe.The establishment of RhD positive blood donor phenotype database can provide timely access to compatible blood for patients facing difficult blood matching challenges.

Key words: RhD positive, Blood donor, Rh phenotype, Phenotype database, Liuzhou