Abstract: Objective To explore the relations between times of delivery and anti-E antibody frequencies in RhE- negative pregnancies. Methods Micro-column gel test was used to detect RhE antigens, irregular antibodies and ABO blood groups in 2012 pregnant women, and spectral cell identification was performed to detect anti-E antibodies in the samples with positive irregular antibodies. Results Fifty percent pregnant women were found to be RhE antigen-negative. No significant difference of positive rates of anti-E antibodies was noted between the women with first pregnancy and those with over one time of pregnancy（P>0.05）. Additionally, no obvious difference of ABO blood group distribution was seen between RhE-negative and –positive pragnancies （P>0.05）. Conclusions Over half of pregnant women present negative RhE antigen and the positive anti-E antibodies are frequently detectable in multipara women, which is significantly meaningful to the women with a second child.

Key words: RhE, Pregnancy, Multipara