Abstract: Objective To determine the level of VEGF and β-HCG in early non-typical ectopic pregnancy （EP） and investigate the combining determination of diagnostic value of VEGF andβ-HCG for EP. Methods 140 pregnancy patients from Nanjing Yuhua hospital were enrolled into this study,of which 60 patients with EP （<7-week）and 80 patients with intrauterinepregnancy. The VEGF andβ-HCG levels were tested by ELISA,ROC was used to cut-off value and all the Results were analyzed by the statistical method. Results The levels of VEGF in EP was significantly higher than that in intrauterinepregnancy,while β-HCG was significantly lower than that in intrauterinepregnancy（VEGF：270.35±111.55pg/ml vs. 125.94±42.38 pg/ml,P=0.000;β-HCG：9 790.35±1 620.79 vs. 12888.13±2 544.41U/ml,P =0.000）. With a cut-off value of 202.50 pg/ml,the AUC value of VEGF is 0.945. The sensitivity of VEGF testing for diagnosing EP was 75.0%,the specificity was 97.5% and the accuracy was 90.2%.With a cut-off value of 10 950.30 U/ml,the AUC value of VEGF is 0.855. The sensitivity of β-HCG testing for diagnosing EP was 76.3%,the specificity was 76.7%,and the accuracy was 83.1%. The sensitivity of the combined testing was 91.3%,the specificity was 95.0% and the overall accuracy was 85.5%. The sensitivity and the overall accuracy of combined testing were higher than those of β-HCG and ADAM12-S testing single. Conclusion EP presents higher VEGF and lower β-HCG concentration than those with intrauterinepregnancy and determination of VEGF and β-HCG is useful to differentiate EP from intrauterinepregnancy. The combined testing of VEGF and β-HCG can increase the sensitivity of diagnosis of EP.

Key words: Ectopic pregnancy, Vascular endothelial growth factor, β-HCG, Diagnosis