Detection of IgG, IgA Antibodies on Red Blood Cells by Flow Cytometry

doi:10.3969/j.issn.1671-2587.2025.01.007

JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE ›› 2025, Vol. 27 ›› Issue (1): 54-58.DOI: 10.3969/j.issn.1671-2587.2025.01.007

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Detection of IgG, IgA Antibodies on Red Blood Cells by Flow Cytometry

LIU Xi¹, SHEN Yuqing², SUN Ying¹, ZHANG Yuyu¹, ZHU Xinyi¹, XIANG Dong¹

¹Shanghai Blood Center, Shanghai 200051;
²Women and Children’s Hospital, School of Medicine, Xiamen University, FuJian Xiamen 361003

Received:2024-11-01 Published:2025-02-25

Abstract

Abstract: Objective In immunohematological tests, IgA blood type antibodies may be missed due to the general lack of anti-IgA. It is necessary to understand the distribution of IgA and IgG antibodies on red blood cells of patients to analyze the potential influence of IgA antibodies on anemia in patients. Methods The Samples collected from patients applying for crossmatching between March and August 2023 were tested for IgA and IgG antibodies using flow cytometry after routine serological testing. Results In 71 cases, 56 IgA antibodies were detected by flow cytometry, which was significantly higher than that reported in domestic and foreign literatures; 56 IgG antibodies were detected. The positive rate was higher than that of conventional serological methods. Conclusion In cases of suspected autoimmune hemolytic anemia or hemolytic blood transfusion reaction with clinical manifestations but negative direct antibody tests, IgA antibody testing should be added to reduce missed detection.

Key words: IgA antibody, Flow cytometry, Immunoglobulin, Autoimmune hemolytic anemia, Hemolytic transfusion reaction

CLC Number:

R446

LIU Xi, SHEN Yuqing, SUN Ying, ZHANG Yuyu, ZHU Xinyi, XIANG Dong. Detection of IgG, IgA Antibodies on Red Blood Cells by Flow Cytometry[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2025, 27(1): 54-58.

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Detection of IgG, IgA Antibodies on Red Blood Cells by Flow Cytometry

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