Abstract: ObjectiveTo investigate the influence of different stored blood temperature on the harvest of leukocyte filtration and the fragility of erythrocyte membrane. MethodsThe leukocyte filter blood bags were used g to collect anticoagulant blood under various blood temperature.The blood was divided into 3 groups before filtration. The temperature of blood of the first group was 8±2℃.The blood was placed in2-6℃for more than 3h,followed by immediate filtration. The blood temperature of the second group was 14±2℃when it was placed at room temperature（RT,24℃）for 30 minbefore filtration. The temperature of blood was 24±2℃,which was placed at RT for more than 4h before filtration. White blood cell counts were determined in each group before and after filtration. Filtration time,erythrocyte osmotic fragility after filtration were determined and analysed. ResultThe time and white blood cell counts after filtration showed significant differences （P<0.05）,the test results of erythrocyte osmotic fragility were statistically significant between the first group and the other two groups （P<0.05）.ConclusionThe recovery of leukocyte filtration and the erythrocyte membrane injuries increased and with the decreased blood temperature. The appropriate temperature of blood storage is 14±2℃.

Key words: Leukocyte, filtration, Blood, temperature, White, blood, cell, Erythrocyte, osmotic, fragility