Abstract: Objective To analyze the HBV poisonous genotype and its mutation of drug resistance in blood donation population in a given area. Methods Selected the blood examples from donors since April 1, 2014 to December 31, 2014 and screened out 201 double positive specimens using ELISA double reagent. And then by fluorescence quantitative PCR, 99 cases of HBV positive specimens were screened out. 45 of them held a viral load larger than 1 000 IU/ml specimens, their HBV drug resistance gene were detected (PCR sequencing method). Finally, we analyzed the relationship between the hepatitis b patient virus poisonous genotype and pills resistance segment mutation of this city. Results All 45 cases was Hepatitis B wild-type, no drug resistance mutation was found. Hepatitis B parting with 45 cases of type B was 43 cases, classification rate was 95.6%, the other 2 cases was type C, classification rate was 4.4%. Type B gene classification rate was significantly higher than C gene classification rate, with statistical significance (χ²=9.256,P<0.05). Conclusion The city's Hepatitis B blood donators hold virus genes model B type at large and the resistance genes are mainly wild type, 45.5% of them has a virus viral load high to 1 000 IU/ml, which need track treatment, providing prevention and cure references for Hepatitis B.

Key words: Hepatitis b virus, Genotypes, Mutation, Drug resistance