Abstract: Objective To studythe pre-storedautologous whole bloodin stead of allogeneic blood transfusionapplied in complex spine surgery and observethe dynamic changes of postoperative inflammatory factors. Methods Fortypatients with complex spine surgeryunder general anesthesia were randomLy divided into autologous group （auto-G） and allogeneic group （allo-G） （n=20）. Patients in allo-G only received allogeneic transfusion whereas patients in auto-G pre-deposited400mLof theirwhole bloodbefore oneweek of operation and received transfusion of intraoperative salvage of shed blood. Serum IL-6，TNF-α，IL-4，and IL-10 were measured immediately after anesthesia（T1），before transfusion（T2），after transfusion（T3），on 1d（T4），4d（T5）and 7d（T6）after operation by ELISA.Blood routine tests were recorded on the days of 1，4，and 7 after operation. Results Postoperative Hb level decreased obviously（P＜0.05）while WBC notably increased in all patients of the two groups（P＜0.05）but no difference was seen between the two groups. On 4 d after operation，IL-6 were significantly higher than that in immediately after anesthesia in two groups（P＜0.05）Serum level of TNF-αand IL-10 on 1d and 4d after operation were significantly higher than thoseon the dayofanesthesia in two groups（P＜0.05）.On 1d and 4 d after operation，TNF-αandIL-10 production in auto-G was lower than that inallo-G（P＜0.05）. On 1d after operation，IL-6 in auto-G was lower than that inallo-G（P＜0.05）whileIL-4 in auo-G was lower than that in allo-G when tested on 4 d and 7 d after operation（P＜0.05）. Conclusion Pre-stored autologous whole bloodcombined with intraoperative autologous blood can reduce the amount of allogeneic blood use to save blood resource，and may help prevent adverse reaction following transfusion.

Key words: Predeposited autologous transfusion , Autologous blood transfusion, Inflammatory factor , Interleukin, Tumor necrosis factor -α