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临床输血与检验

临床输血与检验 ›› 2016, Vol. 18 ›› Issue (1): 59-61.DOI: 10.3969/j.issn.1671-2587.2016.01.018

• 实验技术与方法 • 上一篇    下一篇

汕头献血人群HBV DNA定量检测结果与乙肝两对半模式分析

林树波,肖泽斌,温国强   

  1. 515064 广东省汕头市中心血站
  • 收稿日期:2015-09-10 出版日期:2016-02-20 发布日期:2016-10-10
  • 作者简介:林树波（1977–）,男,广东汕头人,主管检验师,主要从事实验室血液检验工作,（Tel）13501417598（E-mail）lsbtop@163.com。

An Analysis of Correlation of Quanlitative Detection of DNA with Serum Markers in HBV Carriers

LIN Shu-bo, XIAO Ze-bin, WEN Guo-qiang   

  1. Blood Center of Shantou 515064
  • Received:2015-09-10 Online:2016-02-20 Published:2016-10-10

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摘要： 目的分析献血人群中HBV DNA定量检测结果与乙肝两对半模式的关联。方法对于献血者血液筛查出来的HBsAg阳性者进行乙肝两对半检测、HBV DNA荧光定量PCR检测,对于荧光定量PCR高于1000 IU/ml的进行DNA测序。结果所测样本存在9种HBV-M模式,各种模式都有一定比率的DNA活动性复制,测序标本均为HBV B型。结论献血人群的乙肝病毒携带者中存在较高的病毒活动性复制,应予以重视和进一步研究,HBV基因型全部为B型,可能是本地献血人群的分布特点。

关键词: 乙肝病毒, 乙肝两对半, 荧光定量PCR, HBV基因分型

Abstract: ObjectiveTo analyze the HBV-DNA quantitative test results and serum HBV markers in blood donors. MethodsThe positive HBsAg was detected as HBV markers in blood donors and HBV-DNA was tested by fluorescent quantitative-PCR, and samples over 1 000 IU/ml was subjected to DNA sequencing. Results9 HBV-M modes in the measured samples were noted and each of the modes presented the active DNA replication in a certain degree. ConclusionThere are a higher viral replication activity in HBV carriers in blood donors, to which more attention should be paid. The genotype B of HBV might be the endemic character in the local blood donors.

Key words: HBV, HBV, markers, Fluorescent, quantitative, PCR, HBV, Genotype

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