• 中国科学论文统计源期刊
  • 中国科技核心期刊
  • 美国化学文摘(CA)来源期刊
  • 日本科学技术振兴机构数据库(JST)

临床输血与检验 ›› 2017, Vol. 19 ›› Issue (4): 325-327.DOI: 10.3969/j.issn.1671-2587.2017.04.004

• 临床输血 • 上一篇    下一篇

单采血小板保存期内各项生化标志物的动态变化*

石洁, 段志倩, 郑建勇   

  1. 210003 江苏省南京红十字血液中心质量管理科
  • 收稿日期:2017-02-12 出版日期:2017-08-20 发布日期:2017-08-09
  • 通讯作者: 郑建勇,主管技师,主要从事血液产品质量控制与管理工作,(Tel)13813900291(E-mail)417618163@qq.com。
  • 作者简介:石洁(1986-),女,江苏盐城人,检验技师,硕士,主要从事微生物学检验、血液产品质量控制与管理工作,(Tel)13601463830(E-mail)599066727@qq.com。
  • 基金资助:
    *本课题受南京市卫生局一般性项目(No.14171)资助

The Dynamic Change of Various Biochemical Markers during the Preservation of Apheresis Platelets

SHI Jie, DUAN Zhi-qian, ZHENG Jian-yong   

  1. Nanjing Red Cross Blood Center,Quality management Department,210003
  • Received:2017-02-12 Online:2017-08-20 Published:2017-08-09

摘要: 目的 探讨单采血小板在保存期内血小板数量、形态、功能的各项指标变化。方法 采用血细胞计数仪计数血小板数量,使用光镜观察血小板形态,同时测定葡萄糖、乳酸浓度以及血小板P选择素(CD62p)和血小板聚集功能。结果 单采血小板在体外保存过程中,血小板计数和pH值在保存第5天时明显降低;血小板形态学计分在第3天起显著降低;葡萄糖浓度呈逐渐下降,而乳酸浓度呈逐渐升高趋势;血小板聚集功能逐渐降低,保存第3天、第5天与第1天相比差异有统计学意义(P<0.05)。结论 单采血小板在体外保存期间形态和功能发生动态改变,保存3 d内血小板形态和功能基本维持稳定。

关键词: 单采血小板, 保存, 血小板P选择素, 聚集

Abstract: Objective To Analyze and discuss the changes in number,morphology,and function in the preservation period of apheresis platelets.Methods Countand observe the morphologyof platelets while determinethe glucose,lactic acid concentration,platelet P-selectin (CD62p) and platelet aggregation function.Results During in vitro preservation,platelets count and PH value were significantly lower when it comes toDay 5.Platelet morphology score significantly declined since the third day.The glucose concentration gradually decreased and lactic acid concentration showed a trend of rising.Compared with Day1,platelet aggregation function gradually reduced (P<0.05) in Day 3 and Day 5.Conclusion During in vitro preservation period of apheresis platelets,the platelet morphology and function dynamically changed,but were stable in the first three days.

Key words: Apheresis platelets, Preservation, Platelet P-selectin, Aggregation

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