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临床输血与检验 ›› 2020, Vol. 22 ›› Issue (1): 14-17.DOI: 10.3969/j.issn.1671-2587.2020.01.004

• 临床输血 • 上一篇    下一篇

不同浓度二甲亚砜深低温保存实验犬血小板相关参数变化研究*

李丽玮, 于建, 李兴龙, 钟宁, 严赛, 杨燕, 严钱勤, 丁在咸, 李志强   

  1. 200233 上海,交通大学附属第六人民医院输血科
  • 收稿日期:2019-06-18 出版日期:2020-02-20 发布日期:2020-02-28
  • 通讯作者: 李志强,男,主任医师,硕士研究生导师,主要从事血液病学与临床输血学研究,(Tel)021-24058703(E-mail)kcb039@126.com。
  • 作者简介:李丽玮(1987-)女,江苏扬州人,主管技师,硕士,主要从事临床输血血型血清学与分子生物学相关研究,(Tel)021-24058703(E-mail)1527352289@qq.com。
  • 基金资助:
    *本课题受上海市2016年度“科技创新行动计划”实验动物研究领域项目指南(No.16140900301)资助

Study on Changes of Platelet Related Parameters in Dogs with Different Concentrations of Dimethyl Sulfoxide in Cryopreservation

LI Li-wei, YU Jian, LI Xing-long, et al   

  1. Blood transfusion department of Shanghai Jiao Tong University Affillated Sixth People's Hospital,Shanghai 200233
  • Received:2019-06-18 Online:2020-02-20 Published:2020-02-28

摘要: 目的 研究不同浓度二甲亚砜深低温保存实验犬血小板相关参数变化。方法 应用2%和5%二甲亚砜浓度对实验犬血小板进行-80℃深低温保存1~180 d不等,解冻后观察血小板计数(PLT)、平均血小板体积(MPV)、血小板压积(PCT)、血小板分布宽度(PDW)、血小板糖蛋白-Ib(GP-Ib)、血小板选择素(P-Selectin)、血小板因子-4(PF4)及β-血小板球蛋白(β-TG)变化情况。结果 应用2%二甲亚砜浓度和5%二甲亚砜浓度分别深低温保存血小板,保存前后PLT、PCT、GP-Ⅰb、P-Selectin、PF4、β-TG变化均不明显(P>0.05);而MPV、PDW则有不同程度变化(P<0.05)。随着深低温保存血小板时间的延长,在1~180 d内除PLT外MPV、PCT、PDW、GP-Ib、P-Selectin、PF4、β-TG分别具有不同程度变化(P<0.05)。另外,2%二甲亚砜浓度进行深低温保存血小板的PLT、MPV、PCT、PDW、GP-Ib、P-Selectin、PF4、β-TG分别与5%二甲亚砜浓度进行深低温保存血小板比较无明显区别(P>0.05)。结论 在180 d内对实验犬血小板进行2%和5%二甲亚砜浓度深低温保存,对PLT、MPV、PCT、PDW、GP-Ib、P-Selectin、PF4、β-TG相关指标二者间无明显区别。为了减少残留DMSO量导致临床动物试验各种结果影响,推荐可应用2%二甲亚砜浓度进行实验犬血小板深低温保存。

关键词: 实验犬, 血小板, 深低温保存, 二甲基亚砜

Abstract: Objective Study on changes of related parameters in platelet from dogs with different concentrations of dimethyl sulfoxide in cryopreservation. Methods Platelets of experimental dog were prepared with 2% or 5% dimethyl sulfoxide concentrations and stored at -80℃ for cryopreservation from 1 to 180 days. After thawed, the stock of platelet were tested in platelet count (PLT), mean platelet volume (MPV), platelet count (PCT), platelet distribution width (PDW), platelet glycoprotein-Ib (GP-Ib), platelet selectin (P-Selectin), platelet factor- 4 (PF4), β-thromboglobulin (β-TG). Results The platelets after the cryopreservation with 2% or 5% dimethyl sulfoxide concentrations, which differences were observed in MPV, PDW (P<0.05), while the changes of PLT, PCT, GP-Ⅰb, P-Selectin, PF4, β-TG were not significant (P>0.05). With the prolongation of cryopreserved platelet, MPV, PCT, PDW, GP-Ib, P-Selectin, PF4 and β-TG had different degrees of change in 1-180d except PLT (P<0.05). In addition, there was no significant differences between 2% and 5% dimethyl sulfoxide concentration for cryopreservation of platelets in PLT, MPV, PCT, PDW, GP-Ib, P-Selectin, PF4, β-TG (P>0.05). Conclusion The 2 % and 5 % dimethyl sulfoxide concentrations could be chosen for deep cryopreservation of platelets in experimental dogs within 180 days, PLT, MPV, PCT, PDW, GP-Ib, P-Selectin, PF4, and β-TG was no significant difference between the two indicators. In order to reduce the residual amount of DMSO resulting in various effects of clinical animal tests, it is recommended that 2% dimethyl sulfoxide concentration be used.

Key words: Experimental dog, Platelets, Cryopreservation, Dimethyl sulfoxide

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