• 中国科学论文统计源期刊
  • 中国科技核心期刊
  • 美国化学文摘(CA)来源期刊
  • 日本科学技术振兴机构数据库(JST)

临床输血与检验 ›› 2017, Vol. 19 ›› Issue (5): 513-515.DOI: 10.3969/j.issn.1671-2587.2017.05.032

• 试剂与仪器评价 • 上一篇    下一篇

STAR全自动加样系统参数设置优化的探索

蓝文莉, 谢敬文, 陈筱颖, 劳丽嫦, 黎淑贞   

  1. 511400 广东省广州市番禺区中心血站
  • 收稿日期:2017-01-09 出版日期:2017-10-20 发布日期:2017-10-26
  • 通讯作者: 谢敬文,男,主要从事医学检验、输血技术工作,(Tel)13711493203。
  • 作者简介:蓝文莉(1983-),女,广东潮州人,主管技师,学士,主要从事血液检验、输血技术工作,(E-mail)13711493203@126.com。

Parameter Setting Exploration of STAR Automatic Pipetting System

LAN Wen-li, XIE Jing-wen, CHEN Xiao-ying et al   

  1. Guangzhou Panyu District Blood Center,Guangzhou,511400
  • Received:2017-01-09 Online:2017-10-20 Published:2017-10-26

摘要: 目的 探索血站常用酶免设备STAR全自动加样仪加样参数的优化设置,提高加样准确率和工作效率。方法 根据各试剂说明书要求的加样量,设定仪器加样的目标值(target in ),仪器加样后,用质检合格的加样枪对比仪器实际加样量与目标值差距,不断修正,最终设定一个校正值(corrected in)。结果 摸索出血站常用酶免试剂样本及样本稀释液的STAR加样曲线,设定了血站酶免检测各个常用目标值的校正值,并优化了本站8种试剂及留样板的加样顺序。结论 采用厂家设定的通用参数或工程师专为用户设定的专用参数加样实际值可能与目标值存在一定偏差,需要用户根据常规实验的实际加样情况设定校正值、优化加样顺序,确保实验加样准确、高效。

关键词: STAR加样仪, 系统参数, 设置优化

Abstract: Objective To explore parameter settings of ELISA equipment which is commonly used by blood stations on STAR automatic pipetting instrument,in order to improve pipetting accuracy and efficiency. Methods The instrument pipetting target was set according to the sample volume of each reagent required specification. The differences between actual and target amount of sample were compared with quality pipette instrument,and continuously been amended. Finally a correction was set. Results We explored a STAR pipetting curve of ELISA reagents commonly used by blood station,set a correction for each common target value of ELISA detection of blood station,and optimize the pipetting sequence of 8 kinds of reagents tested and left template. Conclusion Using general parameters provided by factory or specific parameters set by engineer for users can cause some deviations between the target values the actual ones. To ensure accurate and efficient,users are required to set the correction and optimize the pipetting sequence according to the actual pipetting volume in the routine experimentation.

Key words: STAR pipetting instrument, System parameters setting, Optimization

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