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临床输血与检验

临床输血与检验 ›› 2017, Vol. 19 ›› Issue (6): 621-623.DOI: 10.3969/j.issn.1671-2587.2017.06.027

• 血型与临床 • 上一篇    下一篇

RhD（-）无偿献血者中RhD表型与基因型检测情况分析*

谈维   

  1. 210003 江苏省南京红十字血液中心中心
  • 收稿日期:2017-07-23 出版日期:2017-12-20 发布日期:2017-12-13
  • 作者简介:谈维（1964-）,男,江苏南京人,副主任技师,主要从事临床输血质量控制与管理工作,（E-mail）183530583@qq.com。
  • 基金资助:
    *本课题受南京市卫生局课题（No; QYK11150）资助

Analysis of RhD Phenotype and Genotype in RhD （-）Blood Donors

TAN Wei   

  1. Nanjing Red Cross Blood Center,Nanjing 210003
  • Received:2017-07-23 Online:2017-12-20 Published:2017-12-13

PDF

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摘要： 目的 寻找一种有效针对无偿献血者的RhD血型基因定型方法。方法 对30例RhD（-）无偿献血者血液样本进行RhD基因定型、弱D 检测和RhCE基因定型。应用荧光定量PCR检测RHD启动子、第4内含子,以及第7、10外显子。结果 30例RhD（-）无偿献血者中,21例未检测到RHD基因,2例为杂交型,其余7例存在RHD启动子、第4内含子和第7、10外显子,其中5例为DEL型,1例为弱D,1例为部分D。30例无偿献血者的RhCE基因型为,20例C-E-c+e+,6例C+E-c+e-,2例C+E-c+e+,1例C-E+c+e-。结论 采用RHCE分型作为Rh（-）定性的筛选检查,能较好地区分D变异体。

关键词: RHD, 基因分型, 无偿献血

Abstract: Objective To built an effective method for RhD genotyping. Methods RhD phenotyping,weak D testing and RhCE phenotyping were performed in 30 samples from members of the RhD-negative club in Nanjing Red Cross Blood Center. The RhD promoter,intron 4,and exons 7 and 10 were analysed by real-time PCR. Results Of 30 RhD-negative persons,21 showed complete deletion of the RhD gene,2 showed the results consistent with RhD-CE-D hybrid,and 7 showed amplification of RhD promoter,intron 4,and exons 7 and 10. Of the latter group,5 were in the DEL blood group,1 were weak D,1 was partial D. The RhD-negative phenotype samples consisted of 20 C-E-c+e+,6 C+E-c+e-,2 C+E-c+e+ and 1 C-E+c+e-. The C-E-c+e+ phenotype showed 100% positive predictive value for detecting D-negative cases.Conclusion RhCE phenotyping is efficient to distinguish D-variants in RhD（-）screening test.

Key words: RhD, Genotyping, Blood donor

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