• 中国科学论文统计源期刊
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临床输血与检验 ›› 2019, Vol. 21 ›› Issue (1): 11-16.DOI: 10.3969/j.issn.1671-2587.2019.01.005

• 气动管道系统传输与血液运送专题 • 上一篇    下一篇

气动管道系统传送全血不同保存时间和温度对血细胞超微结构的影响*

李志强, 于建, 李丽玮   

  1. 200233 上海市第六人民医院
  • 收稿日期:2018-10-23 出版日期:2019-02-20 发布日期:2019-01-28
  • 作者简介:李志强(1962-),男,主任医师,教授,硕士导师,主要从事血液系统疾病诊治与临床输血反应研究,(Tel)021-24058722(E-mail)kcb039@126.com.cn。
  • 基金资助:
    本课题受第四轮(2016-2018年)上海市公共卫生重点建设项目(No.15GWZK0501)资助

Influence of Time and Temperature of Storage in Pneumatic Tube System for Whole Blood Transportation on the Ultrastructure of Blood Cells

LI Zhi-qiang, YU Jian, LI Li-wei   

  1. Shanghai Sixth People's Hospital, Shanghai 200233
  • Received:2018-10-23 Online:2019-02-20 Published:2019-01-28

摘要: 目的 研究气动管道系统传送全血不同保存时间和温度对血细胞超微结构的影响。方法 选取库存时间5 d的全血450 mL/袋,分装为每袋225 mL,采用减震与非减震方法进行PTS传送。应用扫描电子显微镜和透射电子显微镜分别观察传送后不同保存时间和温度条件下血细胞超微结构的变化。结果 无论是否采取减震措施,应用PTS进行保存期5 d全血传送后即刻,对红细胞、血小板、中性粒细胞的超微结构影响不明显。另外,无论是否采取减震措施,经PTS传送后全血分别保存2 h、6 h,随着保存温度变化分别出现不同程度的红细胞超微结构的变化;血小板与中性粒细胞超微结构的变化则不明显。结论 全血经PTS传送后即刻不会导致血细胞超微结构明显的变化,立即输注给患者具有一定的安全性。

关键词: 气动管道系统, 电子显微镜, 血细胞, 超微结构

Abstract: Objective To study the influence of the store time and temperature in pneumatic tube system for whole blood transportation on ultrastructure of blood cells. Method Whole blood were stored for 5 days in 450 ml per bag and each was divided into 225 ml per bag. Damping and non-damping methods for PTS transportation were used. Blood cells were subjected to scanning electron and transmission electron microscopy to observe the cell ultrastructure in various store time and temperature. Result No instant striking difference of ultrastructure of the blood cells, such as RBCs, platelets and neutrophils, were noted between the two methods before PTS transportation. However, the time and temperature after 2 and 6 hr of blood storage following PTS transportation might notably affect the structure of the red cells, which has no close correlation with either damping or non-damping method, meanwhile, there was limited changes observed on platelets or neatrophils. Conclusion Pneumatic tube system has limited impact on ultrastructure of blood cells. Immediate whole blood transfusion after transportation by PTS is safe in clinical transfusion.

Key words: Pneumatic tube system, Electron microscopy, Blood cell, Ultrastructure

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