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临床输血与检验

临床输血与检验 ›› 2018, Vol. 20 ›› Issue (5): 496-499.DOI: 10.3969/j.issn.1671-2587.2018.05.013

• 临床输血 • 上一篇    下一篇

COM.TEC血细胞分离机在儿童外周血干细胞采集中的应用

王嘉怡, 黄儒霖, 梁志敏   

  1. 510623 广东省广州市妇女儿童医疗中心
  • 收稿日期:2018-03-08 出版日期:2018-10-20 发布日期:2018-09-27
  • 作者简介:王嘉怡（1974-）,男,上海人,副主任医师,硕士,主要从事儿童血液/肿瘤及干细胞移植工作,（E-mail）wang-jiayi@163.com。

Harvest of Peripheral Blood Stem Cells from Junior Donors Using COM.TEC Separator

WANG Jia-Yi, HUANG Ru-Lin, LIANG Zhi-min   

  1. Hematology/Oncology Division,Guangzhou Women and Childrens' Medical Center,Guangzhou 510623
  • Received:2018-03-08 Online:2018-10-20 Published:2018-09-27

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摘要： 目的 观察COM.TEC型血细胞分离机在儿童供者行外周血造血干细胞（PBSC）采集过程前骨髓动员、采集中参数、不良反应及采集前后外周血液学指标等变化。方法 用COM.TEC型血细胞分离机autoMNC程序采集53例儿童外周血造血干细胞,年龄（5.0±3.87）岁,体重（20.1±8.36）kg,每次处理抗凝全血（4 045.70±1 167.30）ml,使用抗凝剂（436.1±127.81）ml,时间（213.21±59.88）min。结果 使用粒细胞集落刺激因子（G-CSF）8~10 μg/kg皮下注射进行动员,效果较5μg/kg者优秀,外周血白细胞数明显升高（P=0.01）。53例采集前及采集后白细胞（WBC）数无明显改变,血红蛋白（Hb）、血小板（PLT）计数采集后明显降低（P<0.01）。所有采集者均未发生明显不良反应。结论 COM.TEC型血细胞分离机在儿童PBSC采集过程中,虽然采集后Hb、PLT有一定损失,但所有供者均能耐受,未出现不良反应,数天内均能恢复正常,其工作是安全有效的。儿童PBSC采集的成功完成,除机器参数调整、运行过程白膜的观察外,良好的骨髓动员、血管通路及镇静状态,也是重要的环节之一。

关键词: 血细胞分离机, 儿童, 造血干细胞, 采集, 外周血

Abstract: Objective Peripheral blood stem cells （PBSCs）were collected in children donors by using COM.TEC autoMNC program to observe the changes of bone marrow mobilization,acquisition parameters,adverse reactions and haematology parameters. Methods PBSCs were collected from 53 donors,aged in 5.0±3.8 years and with body weight of 20.1±8.36 kg. The volume of anticoagulated whole blood was 4045.70±1167.30 ml,and 436.1±127.81 ml of anticoagulants and 213.21±59.88 minutes were used in the blood processing. Results Subcutaneous injection of 8-10μg/kg G-CSF was optimal in bone marrow mobilization（P=0.001）,showing a remarkable increase of peripheral granulocytes（P=0.01）.No significant difference of WBCs counts was noted but a decreased Hb and PLTs was seen in 53 cases （P<0.01）. No adverse reactions were observed in all cases. Conclusion The COM.TEC separator is acceptable and efficient for collecting PBSCs of juniors with no adverse reactions although a slight loss of Hb and PLTs might occur. Additionally,efficient mobilization of bone marrow cells and sedative status of the recipients are important in PBSCs collection.

Key words: Blood centrifuge, Children, Hematopoietic stem cells, Collecting, Peripheral blood cells collection

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