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临床输血与检验

临床输血与检验 ›› 2022, Vol. 24 ›› Issue (1): 67-70.DOI: 10.3969/j.issn.1671-2587.2022.01.015

• 临床输血 • 上一篇    下一篇

聚乙二醇对微柱凝胶法检测不规则抗体的增强作用分析

刘晓骅, 罗圆圆, 赵嫚, 李族军, 汪德清, 练正秋   

  1. 100853 解放军总医院第一医学中心输血医学科（刘晓骅,罗圆圆,赵嫚,汪德清）; 成都市第三人民医院输血科（刘晓骅,李族军,练正秋）
  • 收稿日期:2021-07-29 发布日期:2022-01-27
  • 通讯作者: 汪德清,男,博士生导师,主任医师,主要从事临床输血风险控制研究,（E-mail）deqingw@vip.sina.com。
    共同通信作者：练正秋,女,主任技师,主要从事临床合理用血研究,（E-mail）2237932531@qq.com。
  • 作者简介:刘晓骅（1991-）,女,输血医师,硕士,主要从事临床单病种用血及限制性用血策略方面研究,（E-mail） liu422129109@qq.com。

Enhancement Effect of Polyethylene Glycol on Irregular Antibody Detection by Microcolumn Gel Test

LIU Xiao-hua, LUO Yuan-yuan, ZHAO Man, et al   

  1. Department of Blood Transfusion Medicine,First Medical Center,Chinese PLA General Hospital 100853
  • Received:2021-07-29 Published:2022-01-27

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摘要： 目的 探讨聚乙二醇-微柱凝胶法（PEG-MGT）检测不规则抗体的增强作用,及其对不规则抗体鉴定的适用情况。方法 回顾性分析2018年4月~2021年4月低离子强度盐溶液-微柱凝胶法（LISS-MGT）凝集弱造成抗体鉴定不明确而加做PEG-MGT的70例标本,比较两种方法的16系谱细胞反应格局和实验结果。结果 PEG-MGT检出不规则抗体29例（41.4%）：Rh系统相关抗体20例（69%）,Duffy系统相关抗体5例（17.2%）,Kidd系统相关抗体3例（10.3%）和Lewis系统相关抗体1例（3.4%）。格局不明确,特异性待定标本41例（58.6%）,对比LISS-MGT和PEG-MGT反应强度,PEG-MGT增强16例,减弱17例,反应强度无变化8例;通过盐水介质或4℃孵育微柱凝胶法可确定抗体特异性共13例,分别为抗-M抗体9例（PEG-MGT增强4例,减弱3例,强度保持不变4例）,抗-P1抗体2例（PEG-MGT均增强）,抗-Lea抗体（PEG-MGT增强）和抗-Leb抗体（PEG-MGT减弱）各1例。结论 PEG-MGT可显著增强Rh系统、Duffy系统和Kidd系统的抗原抗体反应,对MNS系统、Lewis系统、P1系统等抗体的判断也有一定参考意义。

关键词: PEG, LISS, 微柱凝胶法, 抗体鉴定

Abstract: Objective To explore the enhancement effect of polyethylene glycol microcolumn gel method （PEG-MGT） in irregular antibody detection. Methods We retrospectively analyzed 70 specimens with weak agglutination by low ionic strength salt solution-microcolumn gel method（LISS-MGT） as well as additional PEG-MGT results from April 2018 to April 2021 and compared the 16-pannel cell reaction pattern and experimental results. Results We identified the specific antibodies in 29 cases by PEG-MGT（41.4%）,including 20 cases of Rh system-related antibodies（69%）,5 cases of Duffy system antibodies （17.2%）,3 cases of Kidd system antibodies （10.3%） and one case of Lewis system antibodies（3.4% ）. There were still 41 cases（58.6%）to be determined the specificity of antibodies. By comparing with the reaction intensity of LISS -MGT and the PEG-MGT results,the experimental results showed that PEG-MGT enhanced the reaction intensity in 16 cases,weakened in 17 cases,and unchanged in 8 cases. The antibodies could be determined by saline medium or MGT incubating at 4℃ in a total of 13 cases,including 9 cases of anti-M antibodies（4 cases of PEG-MGT enhancement,3 cases of weakening,4 cases of constant strength）,2 cases of anti-P1 antibody（both PEG-MGT enhancement）,and anti-Lea antibody （PEG-MGT enhancement） and anti-Leb antibody （PEG-MGT weakening） one case each. Conclusion PEG-MGT method could significantly enhance the antigen-antibody response in Rh system, Duffy system and Kidd system, and it also could be helpful to determine antibodies of MNS system, Lewis system and P1 system.

Key words: PEG, LISS, MGT, Antibody, identification

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