人血浆与大鼠同/异源性血浆诱导TRALI大鼠模型比对研究*

doi:10.3969/j.issn.1671-2587.2022.02.011

临床输血与检验 ›› 2022, Vol. 24 ›› Issue (2): 193-196.DOI: 10.3969/j.issn.1671-2587.2022.02.011

人血浆与大鼠同/异源性血浆诱导TRALI大鼠模型比对研究^*

宓文静, 李丽玮, 李兴龙, 李志强

200233 上海交通大学附属第六人民医院

收稿日期:2021-09-07 发布日期:2022-04-12
通讯作者: 李志强,男,主任医师,硕士研究生导师,主要从事血液系统疾病诊治与临床输血反应研究, （E-mail） kcb039@126.com。
作者简介:宓文静（1992-）,女,硕士,主要从事内科血液病基础与临床研究,（E-mail） 1046175231@qq.com。并列第一作者：李丽玮（1987-）,女,主管技师,硕士,主要从事血型血清学和分子生物学研究,（E-mail）alice_517@qq.com。
基金资助:
*本课题受上海市科学技术委员会科研计划项目（No.16140900300、No.17DZ2200800）资助

A Comparative Study of TRALI Rat Model Induced by Human Plasma and Homo/Heterologous Rat Plasma

MI Wen-jing, LI Li-wei, LI Xing-long, et al

The Sixth People' Hospital of Shanghai Jiaotong University, Shanghai, 200233

Received:2021-09-07 Published:2022-04-12

摘要/Abstract

摘要： 目的比较研究人血浆和大鼠同/异源性血浆诱导建立TRALI大鼠模型可行性与有效性。方法 SD大鼠腹腔内注射脂多糖后,分别静脉输注SD大鼠、BN大鼠及人血浆,采集临床症状、肺组织湿干重比、肺组织病理学数据。结果输注人血浆组大鼠均出现明显的TRALI相关临床症状;输注BN血浆组大鼠中只有60%出现不同程度的TRALI相关临床症状;输注SD血浆组大鼠几乎均未出现TRALI相关临床症状（P<0.05）。另外,输注人血浆组大鼠肺组织湿/干重比及肺损伤病理评分与输注SD血浆组大鼠差异有统计学意义（P<0.05）;输注BN血浆组与SD血浆组大鼠肺损伤病理评分差异也有统计学意义（P<0.05）。结论输注人血浆能有效诱导建立TRALI SD大鼠模型,且具有重复性和稳定性。

关键词: 急性肺损伤, 人, 大鼠, 同源性, 异源性, 血浆

Abstract: Objective To compare the feasibility and validity of TRALI rat models induced by human plasma and rat homo/heterologous plasma respectively. Method After intraperitoneal injection of lipopolysaccharide in SD rats, SD rats, BN rats and human plasma were intravenously infused, and clinical symptoms, wet-dry ratio of lung tissue, and lung histopathological data were collected. Result All rats in the human plasma infusion group showed obvious TRALI-related clinical symptoms. Only 60% of the rats in the BN plasma infusion group showed different degrees of TRALI-related clinical symptoms. Almost none of the rats in the SD plasma infusion group showed TRALI-related clinical symptoms（P<0.05）. In addition, the wet-dry ratio of lung tissue and the pathological scores of lung injury in SD plasma group were significantly different from those in human plasma group（P<0.05）. There was significant difference in pathological scores of lung injury between BN plasma group and SD plasma group（P<0.05）. Conclusion Infusion of human plasma can effectively induce TRALI-SD-rat model with repeatability and stability.

Key words: Acute lung injury, Human, Rat, Homologous, Heterologous, Plasma

中图分类号:

R563

宓文静, 李丽玮, 李兴龙, 李志强. 人血浆与大鼠同/异源性血浆诱导TRALI大鼠模型比对研究^*[J]. 临床输血与检验, 2022, 24(2): 193-196.

MI Wen-jing, LI Li-wei, LI Xing-long, et al. A Comparative Study of TRALI Rat Model Induced by Human Plasma and Homo/Heterologous Rat Plasma[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2022, 24(2): 193-196.

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人血浆与大鼠同/异源性血浆诱导TRALI大鼠模型比对研究^*

A Comparative Study of TRALI Rat Model Induced by Human Plasma and Homo/Heterologous Rat Plasma

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