关键词: 抗体鉴定, RhCE复合抗原, f抗原, 抗-f, 抗-M

Abstract: Objective To identify the specificity of antibodies in plasma of patients and develop strategies for cross matching. Methods Antibodies were screened and identified by saline medium test tube method and LISS/ Coombs card, and the specificity of plasma antibodies was determined by the reaction patterns of the Makropanel 16, the Makropanel 16-P and the panel cell at 4℃. Results The patient's Rh blood group was CCDee and MN blood group was NN. Multiple antibody identification results showed that there were alloantibodies in the plasma: anti-f (f:ce composite antigen) of Rh blood group system and anti-M of MNS blood group system. Conclusion Anti-f only reacted with red blood cells containing f antigen (R0, r) and can be enhanced by proteolytic enzymes. The IgG anti-M showed stronger reactivity in the 4℃ environment. After the transfusion and immunization, the patient produced anti-f and anti-M antibodies. In clinical blood matching, red blood cells without M antigen were preferentially selected for cross matching, RhCE antigen was detected according to blood matching results, and red blood cells without f antigen were selected for transfusion.

Key words: Antibody identification, RhCE composite antigen, fantigen, Anti-f, Anti-M