江西南昌地区16例B(A)表型个体的血清学特征和基因型分析*

doi:10.3969/j.issn.1671-2587.2024.02.013

临床输血与检验 ›› 2024, Vol. 26 ›› Issue (2): 239-243.DOI: 10.3969/j.issn.1671-2587.2024.02.013

江西南昌地区16例B(A)表型个体的血清学特征和基因型分析^*

徐晶¹, 舒轶², 杜忻¹, 徐晓琴³

¹南昌市中心血站,江西南昌 330025;
²吉安市中心血站,江西吉安 343000;
³九江职业技术学院,江西九江 332007

收稿日期:2023-11-01 出版日期:2024-04-20 发布日期:2024-04-23
作者简介:徐晶,主要从事分子生物学研究,（E-mail）2270351412@qq.com。
基金资助:
*本课题受江西省卫生厅科技计划项目（No.SKJP220200224）资助

Serological Characteristics and Genotypes of 16 Cases with B(A) Phenotype in Nanchang Prefecture of Jiangxi Province

XU Jing¹, SHU Yi², DU Xin¹, XU Xiaoqin³

¹Nangchang Blood Center, Jiangxi 330025;
²Ji'an Blood Center, Jiangxi 343000;
³Jiujiang Vocational and Technical College, Jiangxi 332007

Received:2023-11-01 Online:2024-04-20 Published:2024-04-23

摘要/Abstract

摘要： 目的探讨江西南昌地区B（A）型个体的血清学特征和基因型。方法收集日常工作中表型为AwB或AB型,但正反血型不一致且高度疑似B（A）型的血液样本16例及1例先证者的家系样本,采用血清学方法进行ABO血型复检;通过对ABO基因6和7外显子测序确定样本的ABO基因型;通过吸收和放散实验,探究B（A）型样本对人源抗A的反应情况。结果 16例血液样本ABO正定型为AwB,血清中存在抗-A。10例样本基因分型为ABO*BA.04,6例样本为ABO*BA.02。吸收放散实验的6例样本中,4例基因型为ABO*BA.04的血样吸收和放散实验均为阴性、2例基因型为ABO*BA.02的血样吸收和放散人源抗-A呈弱阳性。先证者家系调查表明其ABO*BA.04等位基因来自其父亲,并遗传给其儿子。结论 B（A）型个体血清学常表现为AwB的特征,且正反定型不一致,建议结合分子生物学方法鉴定;不同型别B（A）型个体,其细胞表面的A抗原与人源抗-A的反应能力存在差异。

关键词: 血型, B（A）表型, 血清学特征, 基因型

Abstract: Objective To study the serological characteristics and genotype of B(A) phenotype in Nanchang prefecture of Jiangxi province. Methods The phenotype of AwB or AB was collected. ABO blood typing was performed by serological method on 16 highly suspected B(A) blood samples with ABO discrepancies, and family samples from 1 proband. All samples were re-examined by serological method and were subjected to sequence analysis of exons 6 and 7. Absorption-elution tests were performed to detect the reaction of B(A) samples to human anti-A. Results All 16 samples were typed as AwB and weak anti-A was found in serum. Ten samples were genotyped as ABO*BA.04 and 6 samples were ABO*BA.02. Of the 6 samples in absorption-elution tests, 4 samples with ABO*BA.04 genotype were negative, and 2 samples with ABO*BA.02 genotype showed weakly positive with human anti-A. Pedigree study showed that the ABO*BA.04 allele inherited from the father and was passed on to the son. Conclusions The serological characteristics of B(A) phenotype are similar to AwB with ABO discrepancies, so it is recommended to identify them by molecular biological methods. Different types of B(A) individuals have different A antigens on cells and reaction ability to human anti-A.

Key words: Blood group, B(A) phenotype, Serological characteristic, Genotype

中图分类号:

R457

徐晶, 舒轶, 杜忻, 徐晓琴. 江西南昌地区16例B(A)表型个体的血清学特征和基因型分析^*[J]. 临床输血与检验, 2024, 26(2): 239-243.

XU Jing, SHU Yi, DU Xin, XU Xiaoqin. Serological Characteristics and Genotypes of 16 Cases with B(A) Phenotype in Nanchang Prefecture of Jiangxi Province[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2024, 26(2): 239-243.

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江西南昌地区16例B(A)表型个体的血清学特征和基因型分析^*

Serological Characteristics and Genotypes of 16 Cases with B(A) Phenotype in Nanchang Prefecture of Jiangxi Province

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