血小板中细菌病原体检测技术的进展*

doi:10.3969/j.issn.1671-2587.2025.05.013

临床输血与检验 ›› 2025, Vol. 27 ›› Issue (5): 667-673.DOI: 10.3969/j.issn.1671-2587.2025.05.013

血小板中细菌病原体检测技术的进展^*

马静斯, 赵宁, 邓江, 张艳宇

军事医学研究院,北京 100850

收稿日期:2025-09-01 修回日期:2025-09-09 出版日期:2025-10-20 发布日期:2025-10-11
通讯作者: 张艳宇,主要从事输血安全方面研究,（E-mail）swgczhyy@126.com。共同通信作者：赵宁,主要从事血液质量与安全方面研究,（E-mail）zhaoning19901009@126.com。
作者简介:马静斯,主要从事输血方面研究,（E-mail）15620884868@163.com。
基金资助:
^*本课题受国家自然科学基金项目（No.82200738）资助

Progress in Detection Technologies for Bacterial Pathogens in Platelets

MA Jingsi, ZHAO Ning, DENG Jiang, ZHANG Yanyu

Military Medical Research Institute, Beijing 100850

Received:2025-09-01 Revised:2025-09-09 Online:2025-10-20 Published:2025-10-11

摘要/Abstract

摘要： 血小板制品是临床输血的重要成分,但常规（22±2）℃储存易致细菌污染,引发败血症风险,是输血安全的核心挑战之一。本综述旨在梳理血小板中细菌检测技术的研究进展。文章剖析了以自动化微生物培养系统为代表的传统检测方法的优劣,以及对核酸扩增技术、免疫分析法、流式细胞术、质谱技术、宏基因组学、表面增强拉曼光谱、纳米生物传感器等各类技术的性能优劣,解析当前面临的核心挑战,并对未来的发展趋势进行展望,为建立更为完善的血小板质量安全保障体系提供科学依据。

关键词: 血小板, 细菌污染, 检测技术, 输血安全

Abstract: Platelet products are crucial components in clinical blood transfusion. However, their storage under conventional conditions (22±2) ℃ is prone to bacterial contamination, which poses a risk of sepsis and constitutes one of the core challenges to blood transfusion safety. This review aims to summarize the research progress of bacterial detection technologies for platelets. First, the article analyzes the advantages and disadvantages of traditional detection methods represented by automated microbial culture systems, as well as the performance merits and demerits of various emerging technologies, including nucleic acid amplification technology, immunoassay, flow cytometry, mass spectrometry, metagenomics, surface-enhanced Raman spectroscopy, and nanobiosensors. Subsequently, it elaborates on the core challenges currently faced and provides an outlook on future development trends.Ultimately, this review intends to offer a scientific basis for establishing a more comprehensive quality and safety assurance system for platelets.

Key words: Platelet, Bacterial contamination, Detection technology, Blood transfusion safety

中图分类号:

R446.5

马静斯, 赵宁, 邓江, 张艳宇. 血小板中细菌病原体检测技术的进展^*[J]. 临床输血与检验, 2025, 27(5): 667-673.

MA Jingsi, ZHAO Ning, DENG Jiang, ZHANG Yanyu. Progress in Detection Technologies for Bacterial Pathogens in Platelets[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2025, 27(5): 667-673.

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血小板中细菌病原体检测技术的进展^*

Progress in Detection Technologies for Bacterial Pathogens in Platelets

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