富血小板血浆联合间充质干细胞疗法在组织再生中的研究进展

doi:10.3969/j.issn.1671-2587.2025.05.022

临床输血与检验 ›› 2025, Vol. 27 ›› Issue (5): 736-743.DOI: 10.3969/j.issn.1671-2587.2025.05.022

• 综述 • 上一篇

富血小板血浆联合间充质干细胞疗法在组织再生中的研究进展

龚瑾¹, 王海莹², 陈立力², 王辉¹, 张进进^1,2, 邢颜超^1,2

¹新疆医科大学研究生学院,乌鲁木齐 830054;
²新疆军区总医院输血科,乌鲁木齐 830099

收稿日期:2025-09-01 修回日期:2025-09-11 出版日期:2025-10-20 发布日期:2025-10-11
通讯作者: 邢颜超,主要从事临床输血、创面修复、细胞治疗的基础及临床研究,（E-mail）xingyanchao@aliyun.com。共同通信作者：张进进,主要从事临床输血与细胞治疗的基础及临床研究,（E-mail）13319805828@qq.com。
作者简介:龚瑾,主要从事临床检验与诊断学研究,（E-mail）Erin2467@foxmail.com。

Research Progress of Platelet-rich Plasma Combined with Mesenchymal Stem Cell Therapy in Tissue Regeneration

GONG Jin¹, WANG Haiying², CHEN Lili², WANG Hui¹, ZHANG Jinjin^1,2, XING Yanchao^1,2

¹School of Postgraduates, Xinjiang Medical University, Urumqi 830054;
²Department of Blood Transfusion, General Hospital of Xinjiang Military Command, Urumqi 830099

Received:2025-09-01 Revised:2025-09-11 Online:2025-10-20 Published:2025-10-11

摘要/Abstract

摘要： 组织再生是再生医学领域的核心目标之一,目前正面临移植细胞存活率低、功能受限、微环境失衡等关键瓶颈。富血小板血浆（platelet-rich plasma, PRP）联合间充质干细胞（mesenchymal stem cells, MSCs）通过多途径协同作用,提供优化微环境,为解决这一难题提供了新策略。PRP富含的生长因子能显著提高MSCs的存活率、并使其生物学功能得以增强,在多种组织损伤修复中展现协同效应。本文将从PRP调控MSCs功能的分子机制、联合疗法在动物模型中的研究进展及临床应用现状三个方面进行系统阐述,以期为组织再生治疗提供新的理论依据和实践方向。

关键词: 组织再生, 间充质干细胞, 富血小板血浆, 协同效应

Abstract: Tissue regeneration is a central objective in the field of regenerative medicine and is currently confronted with pivotal bottlenecks such as low survival of transplanted cells, limited functionality, and an imbalanced microenvironment. The combination of platelet-rich plasma (PRP) and mesenchymal stem cells (MSCs) offers a new strategy to address these challenges through multi-pathway synergism that optimizes the microenvironment. The growth factors abundant in PRP can significantly enhance MSC survival and function, exhibiting synergistic effects in the repair of various tissue injuries. This article systematically elaborates on the molecular mechanisms by which PRP regulates MSC function, the research progress of the combined therapy in animal models, and the current status of clinical application, with the aim of providing novel theoretical foundations and practical directions for tissue-regenerative treatment.

Key words: Tissue regeneration, Mesenchymal Stem Cells, Platelet-rich plasma, Synergistic effect

中图分类号:

R457

龚瑾, 王海莹, 陈立力, 王辉, 张进进, 邢颜超. 富血小板血浆联合间充质干细胞疗法在组织再生中的研究进展[J]. 临床输血与检验, 2025, 27(5): 736-743.

GONG Jin, WANG Haiying, CHEN Lili, WANG Hui, ZHANG Jinjin, XING Yanchao. Research Progress of Platelet-rich Plasma Combined with Mesenchymal Stem Cell Therapy in Tissue Regeneration[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2025, 27(5): 736-743.

参考文献

[1] XU M M,SU T,JIN X X,et al.Inflammation-mediated matrix remodeling of extracellular matrix-mimicking biomaterials in tissue engineering and regenerative medicine[J]. Acta Biomater,2022,151:106-117.
[2] MAO A S,MOONEY D J.Regenerative medicine: Current therapies and future directions[J]. Proc Natl Acad Sci USA,2015,112(47):14452-14459.
[3] LIANG Z K,HE Y F,TANG H J,et al.Dedifferentiated fat cells: current applications and future directions in regenerative medicine[J]. Stem Cell Res Ther,2023, 14(1):207.
[4] ZHOU T B,LIAO C L,LIN S J,et al.The efficacy of mesenchymal stem cells in therapy of acute kidney injury induced by ischemia-reperfusion in animal models[J]. Stem Cells Int,2020,2020:1873921.
[5] EPANOMERITAKIS I E,KHAN W S.Adipose-derived regenerative therapies for the treatment of knee osteoarthritis[J]. World J Stem Cells,2024,16(4):324-333.
[6] SANTOS L F,SILVA A S,MANO J F.Magnetic-based strategies for regenerative medicine and tissue engineering[J]. Adv Healthc Mater,2023,12(25):e2300605.
[7] GOU Y N,HUANG Y R,LUO W P,et al.Adipose-derived mesenchymal stem cells (MSCs) are a superior cell source for bone tissue engineering[J]. Bioact Mater, 2024,34:51-63.
[8] 龚瑾,张进进,陈立力,等. 人脐带间充质干细胞在膝骨关节炎治疗中的研究进展[J]. 协和医学杂志,2025,16(1): 75-82.
[9] WANG X,XI W C,WANG F.The beneficial effects of intracoronary autologous bone marrow stem cell transfer as an adjunct to percutaneous coronary intervention in patients with acute myocardial infarction[J]. Biotechnol Lett,2014,36(11):2163-2168.
[10] CHAN A M L,SAMPASIVAM Y,LOKANATHAN Y. Biodistribution of mesenchymal stem cells (MSCs) in animal models and implied role of exosomes following systemic delivery of MSCs: a systematic review[J]. Am J Transl Res,2022,14(4):2147-2161.
[11] ACHRAF C,ABDELGHANI P A,EL ANZAOUI JIHAD P. Platelet-rich plasma in patients affected with Peyronie's disease[J]. Arab J Urol,2023,21(2):69-75.
[12] RAMASWAMY REDDY S H,REDDY R,BABU N C,et al. Stem-cell therapy and platelet-rich plasma in regenerative medicines: A review on pros and cons of the technologies[J]. J Oral Maxillofac Pathol,2018,22(3): 367-374.
[13] 袁霆,张长青,余楠生. 富血小板血浆在骨关节外科临床应用专家共识(2018年版)[J/OL]. 中华关节外科杂志(电子版),2018,12(5):596-600.
[14] XU P C,WU Y G,ZHOU L N,et al. Platelet-rich plasma accelerates skin wound healing by promoting re-epithelialization[J]. Burns Trauma,2020,8:tkaa028.
[15] FUKUI M,LAI F Y,HIHARA M,et al.Activation of cell adhesion and migration is an early event of platelet-rich plasma (PRP)-dependent stimulation of human adipose-derived stem/stromal cells[J]. Hum Cell,2024,37(1):181-192.
[16] AIHUA QIN R Z, Heshan Tang, Yu Qiao, et al. Platelet-rich plasma promotes osteogenic differentiation by increasing the opg/rankl ratio in bone marrow stromal stem cells[J]. Journal of Biological Regulators and Homeostatic Agents, 2024, 38(2): 1227-1237.
[17] TEALE M A,SCHNEIDER S,EIBL D,et al.Mesenchymal and induced pluripotent stem cell-based therapeutics: a comparison[J]. Appl Microbiol Biotechnol,2023, 107(14):4429-4445.
[18] CHEN E L,CHEN Z,CHEN L X,et al.Platelet-derived respiratory-competent mitochondria transfer to mesenchymal stem cells to promote wound healing via metabolic reprogramming[J]. Platelets,2022,33(2):171-173.
[19] DAI P X,WU Y,GAO Y X,et al.Multiomics analysis of platelet-rich plasma promoting biological performance of mesenchymal stem cells[J]. BMC Genomics,2024, 25(1):564.
[20] ABUAF O K,YILDIZ H,BALOGLU H,et al.Histologic evidence of new collagen formulation using platelet rich plasma in skin rejuvenation: a prospective controlled clinical study[J]. Ann Dermatol,2016,28(6):718-724.
[21] JI C,ZHANG J H,ZHOU Z X,et al.Platelet-rich plasma promotes MSCs exosomes paracrine to repair acute kidney injury via AKT/Rab27 pathway[J]. Am J Transl Res,2021,13(3):1445-1457.
[22] TRIVANOVIC D,VOLKMANN N,STOECKL M, et al.Enhancement of immunosuppressive activity of mesenchymal stromal cells by platelet-derived factors is accompanied by apoptotic priming[J]. Stem Cell Rev Rep,2023,19(3):713-733.
[23] CHEN Y T,YANG C C,CHIANG J Y,et al.Prion protein overexpression in adipose-derived mesenchymal stem cells (ADMSCs) effectively protected rodent kidney against ischemia-reperfusion injury via enhancing ATP/mitochondrial biogenesis-role of ADMSC rejuvenation and proliferation[J]. Cell Transplant,2023,32:9636897231211067.
[24] HOSACK G R,ICKOWICZ A,HAYES K R.Quantifying the risk of vector-borne disease transmission attributable to genetically modified vectors[J]. R Soc Open Sci, 2021,8(3):201525.
[25] ZHANG J H,ZHANG J,ZHANG N N,et al.The effects of platelet-rich and platelet-poor plasma on biological characteristics of BM-MSCs in vitro[J]. Anal Cell Pathol,2020,2020:8546231.
[26] CHEN J F,ZHANG Y Y,LIU M J,et al.Effect and related mechanism of platelet-rich plasma on the osteogenic differentiation of human adipose-derived stem cells[J]. Biomed Res Int,2022,2022:1256002.
[27] PARASCANDOLO A,DI TOLLA M F,LIGUORO D,et al. Human platelet-rich plasma regulates canine mesenchymal stem cell migration through aquaporins[J]. Stem Cells Int,2023,2023:8344259.
[28] HERSANT B,SID-AHMED M,BRAUD L,et al.Platelet-rich plasma improves the wound healing potential of mesenchymal stem cells through paracrine and metabolism alterations[J]. Stem Cells Int,2019,2019: 1234263.
[29] ZHOU G H,TAO M,ZHU J,et al.Hypoxia preconditioned plasma increases the expression of growth factors to accelerate diabetic wound healing by promoting angiogenesis[J]. Sci Rep,2025,15(1):23154.
[30] TANG S J,CHEN P H,ZHANG H R,et al.Comparison of curative effect of human umbilical cord-derived mesenchymal stem cells and their small extracellular vesicles in treating osteoarthritis[J]. Int J Nanomedicine, 2021,16:8185-8202.
[31] PERRY J,ROELOFS A J,MENNAN C,et al.Human mesenchymal stromal cells enhance cartilage healing in a murine joint surface injury model[J]. Cells,2021,10(8): 1999.
[32] PAN X,LI X F,ZHANG L,et al.Umbilical cord mesenchymal stem cells relieve osteoarthritis in rats through immunoregulation and inhibition of chondrocyte apoptosis[J]. Sci Rep,2023,13(1):14975.
[33] LI F,LI Q,WANG L F.Advances in the research of effects of mesenchymal stem cells,exosomes,and platelet-rich plasma in wound repair[J]. Zhonghua Shao Shang Za Zhi,2019,35(10):764-768.
[34] LI C,HUANG J X,ZHU H S,et al.Pyridoxal-5'-phosphate promotes immunomodulatory function of adipose-derived mesenchymal stem cells through indoleamine 2,3-dioxygenase-1 and TLR4/NF-κB pathway[J]. Stem Cells Int,2019,2019:3121246.
[35] 中华医学会风湿病学分会,中国医院协会临床新技术应用专业委员会. 异体间充质干细胞治疗系统性红斑狼疮专家共识[J]. 中华风湿病学杂志,2022,26(1):1-8.
[36] WU B H,SU S Y,WANG X Y,et al.Bone marrow mesenchymal stem cell-derived exosomes alleviate DSS-induced inflammatory bowel disease in mice through inhibiting intestinal epithelial cell pyroptosis via delivery of TSG-6[J]. Front Immunol,2025,16: 1601591.
[37] LIU Y Y,ZHANG X D,HU Y W,et al.Human placental mesenchymal stem cells regulate inflammation via the NF-κB signaling pathway[J]. Exp Ther Med,2022, 24(5):654.
[38] REN X Y,ZHONG W L,LI W T,et al.Human umbilical cord-derived mesenchymal stem cells alleviate psoriasis through TNF-α/NF-κB/MMP13 pathway[J]. Inflammation, 2023,46(3):987-1001.
[39] MATA M,SALVADOR-CLAVELL R,RÓDENAS-ROCHINA J,et al. Mesenchymal stem cells cultured in a 3D microgel environment containing platelet-rich plasma significantly modify their chondrogenesis-related miRNA expression[J]. Int J Mol Sci,2024,25(2):937.
[40] LIM H C,PARK Y B,HA C W,et al.Allogeneic umbilical cord blood-derived mesenchymal stem cell implantation versus microfracture for large,full-thickness cartilage defects in older patients: a multicenter randomized clinical trial and extended 5-year clinical follow-up[J]. Orthop J Sports Med,2021,9(1): 2325967120973052.
[41] Zhou Y,Zhao Y,Wu Y,et al.Human umbilical cord mesenchymal stem cells alleviate rat knee osteoarthritis via activating Wnt/β-catenin signaling pathway[J]. Curr Stem Cell Res Ther,2024,19(2):234-244.
[42] MOHAMMED R N,AZIZ SADAT S A,HASSAN S M A,et al. Combinatorial influence of bone marrow aspirate concentrate (BMAC) and platelet-rich plasma (PRP) treatment on cutaneous wound healing in BALB/c mice[J]. J Burn Care Res,2024,45(1):59-69.
[43] RAMOS-GONZALEZ G,SALAZAR L,WITTIG O,et al.The effects of mesenchymal stromal cells and platelet-rich plasma treatments on cutaneous wound healing[J]. Arch Dermatol Res,2023,315(4):815-823.
[44] MOHAMED N H,SHAWKAT S,MOUSSA M S,et al.Regeneration potential of bone marrow derived mesenchymal stem cells and platelet rich plasma (PRP) on irradiation-induced damage of submandibular salivary gland in albino rats[J]. Tissue Cell,2022,76:101780.
[45] EBRAHIM N,DESSOUKY A A,MOSTAFA O,et al.Adipose mesenchymal stem cells combined with platelet-rich plasma accelerate diabetic wound healing by modulating the Notch pathway[J]. Stem Cell Res Ther,2021,12(1):392.
[46] TANG Y,WANG H P,SUN Y L,et al.Using platelet-rich plasma hydrogel to deliver mesenchymal stem cells into three-dimensional PLGA scaffold for cartilage tissue engineering[J]. ACS Appl Bio Mater,2021,4(12): 8607-8614.
[47] ALMANSOORI A A,KWON O J,NAM J H,et al.Mesenchymal stem cells and platelet-rich plasma-impregnated polycaprolactone-β tricalcium phosphate bio-scaffold enhanced bone regeneration around dental implants[J]. Int J Implant Dent,2021,7(1):35.
[48] XIAO W F,YANG Y T,XIE W Q,et al.Effects of platelet-rich plasma and bone marrow mesenchymal stem cells on meniscal repair in the white-white zone of the Meniscus[J]. Orthop Surg,2021,13(8):2423-2432.
[49] GHAFFARINOVIN Z,SOLTANINIA O,MORTAZAVI Y,et al.Repair of rat cranial bone defect by using amniotic fluid-derived mesenchymal stem cells in polycaprolactone fibrous scaffolds and platelet-rich plasma[J]. Bioimpacts,2021,11(3):209-217.
[50] ZHOU Y,SHEN H X,WU Y L,et al.Platelet-rich plasma therapy enhances the beneficial effect of bone marrow stem cell transplant on endometrial regeneration[J]. Front Cell Dev Biol,2020,8:52.
[51] TRIPATHI T,MOHAN S,ALFAIFI H A,et al.Efficacy and safety of stem cell therapy for fistula management: an overview of existing systematic reviews[J]. Int J Surg, 2024,110(12):7573-7584.
[52] LIANG S,ZHENG Z Q,LI Y X,et al.A review of platelet-rich plasma for enteric fistula management[J]. Front Bioeng Biotechnol,2023,11:1287890.
[53] BERAN A,GHAZALEH S,SAYEH W,et al.S191 Efficacy and safety of adipose-derived stem cell therapy for the treatment of complex perianal fistula not associated with Crohn's disease: a systematic review and meta-analysis[J]. Am J Gastroenterol,2022,117(10S): e139.
[54] 刘嘉浩,李璐蓉,张伟锋,等. 内镜下注射自体富血小板血浆治疗难治性上消化道瘘8例临床分析[J]. 中华消化杂志,2023,43(11):741-746.
[55] SUN Y Q,ZHU M,QIU L M.Efficacy of mesenchymal stem cells and platelet-rich plasma therapies on wound healing: A Systematic Review and meta-analysis[J]. Regen Ther,2025,30:75-91.
[56] 赵丽坤, 陈婷婷, 梁峰, 等. 1例同种异体富血小板血浆在直肠吻合口瘘治疗中的应用[J]. 临床输血与检验,2023, 25(5):685-687.
[57] BÄCHLER T,POZZA G,GINGERT C,et al. Regenerative therapy with autologous stromal vascular fraction derived mesenchymal stem cells and platelet-rich plasma to treat complex perianal diseases[J]. Br J Surg,2023, 110(Supplement_5):znad178.037.
[58] DEBS T,IANNELLI A,FREY S,et al.Mesenchymal stem cells and platelet rich plasma therapy to treat leak after sleeve gastrectomy[J]. J Surg Res,2021,268:405-410.
[59] ZHANG Y Y,YI D,HONG Q,et al.Platelet-rich plasma-derived exosomes enhance mesenchymal stem cell paracrine function and nerve regeneration potential[J]. Biochem Biophys Res Commun,2024,699:149496.
[60] TATSIS D,VASALOU V,KOTIDIS E,et al.The combined use of platelet-rich plasma and adipose-derived mesenchymal stem cells promotes healing. a review of experimental models and future perspectives[J]. Biomolecules,2021,11(10):1403.

富血小板血浆联合间充质干细胞疗法在组织再生中的研究进展

Research Progress of Platelet-rich Plasma Combined with Mesenchymal Stem Cell Therapy in Tissue Regeneration

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