洗涤红细胞蛋白组学和代谢组学分析及关键质量评价的研究*

doi:10.3969/j.issn.1671-2587.2026.04.006

临床输血与检验 ›› 2026, Vol. 28 ›› Issue (4): 496-503.DOI: 10.3969/j.issn.1671-2587.2026.04.006

洗涤红细胞蛋白组学和代谢组学分析及关键质量评价的研究^*

苏晓敏, 张国权, 王雅琼, 徐华

陕西省血液中心,西安 710061

收稿日期:2026-07-08 出版日期:2026-08-20 发布日期:2026-08-26
通讯作者: 徐华,主要从事输血医学和血型检测方面的研究,（E-mail）drxuhua@163.com。
作者简介:苏晓敏,主要从事输血医学和血液成分研究工作,（E-mail）sufeichi112@163.com。
基金资助:
^*本课题受西安市卫生健康委员会科研项目面上培育项目（No.2026ms05）、西安市卫生健康委员会科研项目一般研究项目（No.2026yb04）资助

Proteomic and Metabolomic Analyses of Washed Red Blood Cells with Key Quality Assessment

SU Xiaomin, ZHANG Guoquan, WANG Yaqiong, XU Hua

Shaanxi Blood Center, Xi'an 710061

Received:2026-07-08 Online:2026-08-20 Published:2026-08-26

摘要/Abstract

摘要： 目的本研究旨在对洗涤红细胞（washed red blood cell, WRBC）质量、蛋白组学及代谢组学相关参数展开测定。同时,通过关联分析,深入评估其储存期末质量变化情况。方法将悬浮红细胞分为2组,分别为未洗涤对照组（Control5w）和生理盐水洗涤组（Saline5w）。4 ℃保存后,于储存期末取样并检测悬浮红细胞质量控制的相关指标。开展蛋白组学和代谢组学检测,通过生物信息学分析评价两组悬浮红细胞储存过程中相关生物学功能变化情况及相关性。结果 Saline5w组红细胞4 ℃保存35 d后,其各项指标符合WRBC相关国家质量标准。进一步对蛋白组学和代谢组学数据进行整合分析,通过探究不同层次生物分子之间的关联,发现经生理盐水洗涤的红细胞,其蛋白质和代谢物组成发生了一定的变化。结论本研究通过蛋白组学和代谢组学检测手段的应用,为洗涤RBC生理生化功能等方面的研究提供了有力支持。

关键词: 洗涤红细胞, 蛋白组学, 代谢组学, 多组学联合分析, 质量评价

Abstract: Objective This study aimed to determine the quality indicators, proteomic and metabolomic profiles of washed red blood cells (WRBCs), and further evaluate their quality alterations at the end of storage via correlation analysis. Methods Suspended RBCs were categorized into two groups: the control group (Control5w), which did not undergo washing treatment, and the saline-washed group (Saline5w). Following storage at 4 ℃, samples were collected at the end of storage, and relevant quality control indicators for suspended RBCs were examined. Proteomic and metabolomic analyses were performed to assess changes in biological functions and correlations during the storage process of the two groups using bioinformatics analysis. Results After 35 days of storage at 4 ℃ in the saline group, all RBC indicators complied with national standards for the quality of WRBCs. The comprehensive integration and analysis of proteomics and metabolomics data have elucidated alterations in the protein and metabolite composition of RBCs washed with saline. This was achieved by examining the correlations among various biomolecular levels. Conclusion This study substantiates the investigation of the physiological and biochemical functions of washed RBCs through the application of advanced proteomic and metabolomic methodologies.

Key words: Washed red blood cells, Proteomics, Metabolomics, Integrated multi-omics analysis, Quality evaluation

中图分类号:

Q493.2

苏晓敏, 张国权, 王雅琼, 徐华. 洗涤红细胞蛋白组学和代谢组学分析及关键质量评价的研究^*[J]. 临床输血与检验, 2026, 28(4): 496-503.

SU Xiaomin, ZHANG Guoquan, WANG Yaqiong, XU Hua. Proteomic and Metabolomic Analyses of Washed Red Blood Cells with Key Quality Assessment[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2026, 28(4): 496-503.

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洗涤红细胞蛋白组学和代谢组学分析及关键质量评价的研究^*

Proteomic and Metabolomic Analyses of Washed Red Blood Cells with Key Quality Assessment

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